miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: Browse




mature miRNA: TGACAGAAGAGATGGAGCATG
species: Nicotiana tabacum
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|190143818|gb|FG644127.1|FG644127 TT-17_C10 K326 late senescent leaf library Nicotiana tabacum cDNA, mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||.|||||.|||| .
CCTGTCTTTTCTACTTCGTCT

EST sequence (targeted fragment marked with yellow):
GAAGTTTTTTGGGGTCTTGATTTTCTTTCCACAGGAGAAAAAAACAAAGAAAGAGAGAACCCTAGGAGGAAAACCCACGGACTGTTCATCTTCTTCATCTTTGAAGAAGAGGGGACACGACGGAAGACCCTCAAAAAACGACCCCTTCGAAACTCCATCCTCGCGCACCACCATACACATCGCGTCGACCACCCCGTCGAGCAGCAGTTGCTGCAGCTGCTTCACGTCCTTCCCCCATCGTCTTCCCCACCTCTAAACCTCTCCGTAACAACCAGCAAACGACCCTTTTTGTCGCCGGAAAAACCAGTAGCTCGCCTTCTTCCTCCATTGAAACCAACTCCATCCTCACGTCCAGCGATCACGACCAGACTCCCCGTCGCTTCCTCGCGCACATCAGTGAACCAAACAGTCCGGCGACCACAACTCGTCTCCCGTCATCACCATCGTCCCCAGCTGCTCGTCGCAGCTGCTTCTGCCTTCTGCTTCATCTTTTCTGTCCAAACGACCGCGTCGCCATAACCAGCCCCAACTCTCACCGTCGTCCATCACCCGCGAACACCTCCATAACCCACGACCCCTCGTTTTTTTAGCAGCAGCAACCACTGCTGCATTATCGACCATACTCCTGTCCAAACTTCCTTCGTCGACCACAAGCGACGCCCAGNNCCGGTCGTCTACCAGTCAGCTTCACGNNAAGCAGCACTGNCGGCTGTTTCCCC



gi|190769454|gb|FG201542.1|FG201542 AGN_PNL231cr1_a8.trimmed.seq AGN_PNL Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||.||||||...||| |.
ACTGTTTTCTCTGTTTCGGAT

EST sequence (targeted fragment marked with yellow):
GCTGTTGCTGATGCTTTAGCTGCCACTGATAAGAAGCAGCAGCAGCTTAGCTCTAGCTTGGATTCAGCTCCAGAGAGGGATGAGAATGTGGTGCATATGAGTTCTCCAAAAGATCCTGATCGGCATGAATCGGGGCTGGATATGTCAAAGCAGAAGCCCAGGGAACCTAGATCTCCGGTCAGCTTTCGAGCGTGGAGGCCTGATGATGCATTCCGTCCACAAAGTCTTCCGAATTTATCGAAACAGTATACTTTCCCTATGAATTTGGGGCGGCATTACCGGGGAGGCTCTGTATGGGGATGTAAAAGTTTATCAATACCCACATCATGTCCTATATGCTGTGAGGATTTGGATCTTACAGACACAAGCTTTCTTCCTTGTCCTTGCGGGTTTAGGCTTTGTCTCTTTTGTCACAAGAGGATTCTTGAGGAGGATGGGCGGTGTCCAGGTTGCAGGAAGCAGTATAAACATGCTTGAGGGAGAGACAACCTAAGATGCAGGCAGTCTGACATTTCGACCGACTTGTTCTTGTAGCATGATATCAAGGTCGTAGGAACATTCTGTTTTCTGTGAATGTGTATGTGTCGTCCTCTTTCTCCGTGTGGCTTGACTTTGTTAGACTCAGATTTTTCTTAGCATTAGATGTTGATATAGGACCGAGATTCTGAAAGCACGGGGGAACTGTTATGGTTCTCTTATAATTTTGCTAATTGTAATATGGTGCTTGTGCGGATGTAGAATA



gi|190799566|gb|FG195221.1|FG195221 AGN_PNL222dr1_d11.trimmed.seq AGN_PNL Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||||||||||||||.
ACTGTCTTCTCTACCTCGTGA

EST sequence (targeted fragment marked with yellow):
TGAGTGCATCAAAGTTCTTGGACCTACCCTTCTGATCATGTAATACAAACACACACATCACATTCAAACAACAATGATACATGAAAAAACCACTACATCAGGACAATAGTTTCAGCAATATCTCATGGACTCATCAGTGCTCTGGTTCACATGCCATATGAACACACTATTGAGAACTGGTTATGGGCACATCAGAGTCTCTACAACAGCCAGTCACAACCACCAATGCAGTGCTCCATCTCTTCTGTCATGGACACAAAACACAGACAACTCATTGTTCTTCATCTTTTGGACATCTATATGCTTCTCTTCAACCCGACTTTCCTTGTTGGCAATGCTAAAGCTATGATGAAGATAGGTTCCTGGTGTTATAGAACTAACCCCATTCTTGGATGCATATTCTTTTATTGTCACTTCATTTGGGATAATTTGTCCAAACTTTAGATATAGTTCCTCCTATAAGTGGAAAGAGTCTATCCAAATTTTTTGCGCAGTTGACCTAGGTTACTCTTATTTTTGGAGTCCATTTGCTTACCCAATATGGATAGGCATATGGTACCATCTTATAGGTCTAAATTCACTTATTGTACATGATTCAATTGAGGGTCTTTTTTTAAACTATCAATTGATTATCCACCATAGGTTTGGAAGTAAGGTTCTGTCCCCCTCTCGATGGTGTAAATATTGGTTTCATATTTAAAAAACTAG



gi|254642096|gb|FS433954.1|FS433954 FS433954 normalized full-length tobacco cDNA library Nicotiana tabacum cDNA clone TBK02GR009

TGACAGAAGAGATGGAGCATG
||| ||||||||.|||| |..
ACTTTCTTCTCTGCCTCTTGT

EST sequence (targeted fragment marked with yellow):
GACAAAAACCCATTTTCCCCTTTANTTTCTCCAACATAATTGAGGTTCAATTCTAAAGCCAAAGAAATGGGTCGTCTAAATCTTCTTATAGCAGTAGTGTTATCAATATATTCAGTTGGTGTAGTGTTCATGCCCAACTTGGCTTTTGCACAACTGAAGACCAATTATTACGCCAAAACCTGTCCTAATGTCGAATCCATTGTTAGAAATGTGGTTAACCAGAAATTCCAACAAACATTTGTCACAATCCCTGCTGTTCTCCGTCTCTTCTTTCATGATTGCTTTGTTGAGGGTTGTGATGCTTCAGTGATAGTATCGTCAACACCAGGGAACACAGCTGAGAAGGATCACCCAGATTATCTATCATTGGCAGGAGATGGATTTGATACTGTGATCAAAGCCAAAGCCGCCGTTGATTCAAAACTCACGTTGTAAAAATAAAGTCTCTTGTGCAGATATTCTCGCCTTAGCCACTAGAGACGTCATTCAAGCTGTCGGGGGGGCCATGGTACCCAGTAGAATTAGGAAGGCTAGATGGGTTCACATCACAAGCTTCAAAACGTGGA



gi|190733812|gb|FG170265.1|FG170265 AGN_RNC002xi04r1.ab1 AGN_RNC Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
||||||||.|| || |||||
ACTGTCTTTTCGACGTCGTAG

EST sequence (targeted fragment marked with yellow):
GTGAGTACCACTTCTGCAGTGCTATTAAGTGATCAGTGGTGGTCGCTGGATGCTGCAGCTTTTCTGTCATCAGTAGTCTGCATACGAACTGTGAAACGTTATGATTTGAGATGCACAGCAGTGTCTATGCATGAATCTTGGGCTTGCCCCAGTTTAGGACTGGTACGTGTCCCGGGGATAGGACTTGTCTACACCGAGCTGGCTAGTGGGGTGCCACACATCTTTTCTCACTTCATTACAAATCTGCCAGCTATACATTCAGTTGTGGTATTTGTCTGTGTGAAGTATCTTCCAGTTTACACAGTTCCAGAAGATGAGAGGTTCCTCGTGAAACGCGTAGGACCCAAGACTTTTCACATGTTCCGCTGTGTTGCAAGGTATGGTTACAAAGACCTCCATAAGAAAGATGAGGAGTTCGAGAGAAAGCTCTTTGATAATCTCTTCCTATTTGTCCGGCTGGAGAATATGATGGAAGGCTGCTCTGACTCTGATGAATACAGCTTATACGGACAGCAAACGCAGAATTCGATGGATTATTTACTGCAAAATAAGAGCAACCCAACTACAGGAAATAATACATGTTCGTCGATGGAATCAATAGTACCCGTAAAATCTCCCACTCAAGGAAGCAATACAGTCACATCATCATCAGGCCGTGAGAGCAGCCAGGCAGAAGTGGACGAATTAGAATTCTTGAATCGTTGTCGAGATGCTGGGGTTGTACACATTCTTGGAAACACTGTAGTTAGAGCAAGGAGAGACTCTAGGTTCTATAAGAAAATTGCTATCGACTATATATATGCGTTTCTTAGGAGAATATGCAGGGAAAATAGTGTGATCTTCAACGTACCTCATGAGAGCCTCTTGAACGTTG



gi|156653098|gb|AM828626.1|AM828626 AM828626 DL, diurnal library Nicotiana tabacum cDNA clone nt005044053, mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||.||.|||||||.|||.
ACTGTTTTTTCTACCTTGTAT

EST sequence (targeted fragment marked with yellow):
AAAATAAATTTAATCTTTAAACATTTTTTCTTTTAGTTTTGTTGAGTGAAAGGACTTCCTTTGATATGGCTTCCGTTGATGAGGATGTTAACTCTGTATGTTCCAGTTCCTGGGAGATTGTAATTTACGAGGTATGTTCCATCTTTTTTGTCAGATACTTCGACACCTTGTACTTCACCTTTGCTTCCTTGAATTTTTACATCAATATGATCTCTTTCCCCACCAGAAGTCAATCTCTTACCAGTCTTAAAGCAAGTTCTAATTACAAAAGAGTAACCATCAATGAAAGAGTTTCCTGCATCAGCACCTTCAATTACATTAACGTTGTATGGAGAGTTTGCAACTTGAT



gi|190747015|gb|FG190458.1|FG190458 AGN_PNL216br1_c7.trimmed.seq AGN_PNL Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
||||||||.||| .|||||..
ACTGTCTTTTCTCTCTCGTGT

EST sequence (targeted fragment marked with yellow):
AACAAGAAGCAAAACTAATGAGAGTAGCAACTCATATCTGAACGATATTGAAGGATCAGATTTTGTTTCCCGTTCTCTGTTCCACAACACCTCAATAGGGAGCGAGGTCTTGAATGTTATGGACTCGGCCTCTACTTTTCAAGGGATATCAAACTCTGGCTGTGCTCTCTCTTTTCTGTCATCTCAATCACAAGACTCGTCAAATTATTCATCTCTAGTTCCTAATGCACACCACCTTATTACACCTCGCAGTAATGCCCATTACAATGTGACACAGACATTTGAAAAGTTCCCAGGGGTGAACCTGAAGGATTCAACAAGTAATGCGTCCAGTATATTCAATTTATCCGGAGCAATTTTTGCTGAGGATGGCATGGAACAGATACTAAGAAACAACTGTGGCATCAACGGGAGTATTCACGGATCAGATTATGTGAACACCAAGAATTTACTTTCTTGTGAAGATGGTCCTACTATTGATTTGCTTCAGCTGTTGTTTCAGCTTCATCGAGTGGAGCATCAAAGACATTTCATGAAATGAAGCAAAATGGCGACACTTTTTGCCAGCCTTTGGATCACTTGACCAGGTAACTGAATAATGTGCCTACCACAATGAGAGTTTTATTATGTTTCCAAGTGAAGTATGTAAGATTTTCGTTTTTGCTGGATGATAACATTGAATACCACTTAATGGCATATGACAATCCTCTTA



gi|156696670|gb|AM810573.1|AM810573 AM810573 seedling library, SL Nicotiana tabacum cDNA clone nt002214089, mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||.|||||||||| |..
ACTGTTTTCTCTACCTACTGT

EST sequence (targeted fragment marked with yellow):
AAAACTTAATCAAGATGCGTGTCTTTCTGTTTTATTATTAGTTTTAGTTGCATTAGCATTCGTATCTGCAGCTGACCCCACCCCTGAACAAGCCCAATGTATGGCTTCAAAATGTATGACTGAAGTTCAACAATGTGCCACCAAATGTTCAAACAGTAATGATTGGAAATGTTATGAAGCCAATTGTGGTCCATTTGTTACTTGTGCAAAGAACCAATGTGGTTATACCATTTCCAATGATGCTTCTACTACATCTCTCTCCATGGGAGCTCTCGCTGTCGTTGGAGCTATGGCAGTCATGCAAGCACTTTAAATTGTCCAAGACAACACATACCCAACATATCATCTCAACAATGTCATCTCAACAATGTGAATTGTTGTTGTTTATCATTTGTTTGTTTGTGTCATGTCATGTTTGTCATCCATCTCTTTTGTCATCAAGAGATCTATCATCCAGCTCTATCTATCTTTATAGTTAGATCAGAGATGTTGCGTGCATGTGTCGAGTGGATTAATGTCATTCACCCAT



gi|190878339|gb|FG197539.1|FG197539 AGN_PNL226ar1_a5.trimmed.seq AGN_PNL Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||.||||||...||| |.
ACTGTTTTCTCTGTTTCGGAT

EST sequence (targeted fragment marked with yellow):
CGATTGGGAGGCTGTTGCTGATGCTCTGGCTGCCACTGATCACAAACAGGAGCAGCATAATCCCAGCTTGGATTCACATTGTGAGAGGGATGATAATGTTGCGCATATGAGTTCTAGGCAAGTGACTTCTAACAGGCAGGTTTCAGAGTCTAATGACAGAGGATTGGCACCTAGACCTCCTCTCAGTTCCCAGGCATGGAGGCCTGATGATACCTTCCGTCCACCGAGTCTGCCAACCTTGTCGAAGCAGTATAGTTTTCCAATGAATTCTGAGCGGCATTGTCGGGGAGGCTCTGTATGGGGATGTAAAAGTTTATCCTCCATACCCACGTCATGTCCTATATGCTGTGAAGATTTGGATTTTACAGACACAAGTTTTCTCCCTTGTTCATGTGGGTTTAGGCTTTGTCTCTTTTGTCACAAGAGGATTCTTGAGGAGGATGGGCGTTGCCCCGGGTGCAGGAAGCAGTATGTTCAGGATCCAGTCGAGCGAGAGGCTACCATAGATGGAGGCAGCCTGACAGTTCGATTGACTCGTTCTTGTAGCATGATATCAAGGTCCTAGGAAGATTTTGTTTTTCCATGAATGTGTATGTTGGGTCCTTTAAAGTCTCTGCATGACATGTGAGTGTTCTAGCTCTTAGCAGACTGAGACTCAAGATTTTCCATATCTTAGATGTTGATATAGGTGAGTCTGAAAGCATGGAAAAATAGTTCTGGTTCTCTTATGATTTTTTGCATTATAGATGTGGTGCTTGTGCTGATGTAGAACACT



gi|254655561|gb|FS418331.1|FS418331 FS418331 normalized full-length tobacco cDNA library Nicotiana tabacum cDNA clone TBK02GR004

TGACAGAAGAGATGGAGCATG
||| ||||||||.|||| |..
ACTTTCTTCTCTGCCTCTTGT

EST sequence (targeted fragment marked with yellow):
GGCAAAAACCCCATTTTCCCCTTTANTTTCTCCAACACAATTGAGATTCAATTCTATAGACAAAGAAATGGGTCGTCTAAATTTTCTTATGGCAGTAGTGTTGTCAATATATTCCGTTGGTGTAGTGTTGATGCCCAACTTGGCTTCTGCACAACTGAAAACCAATTATTACGCCAATACCTGTCCTAATGTTGAATCCATTGTTAGAAATGTGGTTAACCAGAAATTCCGACAAACATTTGTCACAATCCCTGCTGTTCTCCGTCTCTTCTTTCATGATTGCTTTGTTGAGGGTTGTGATGCTTCAGTGATAGTATCATCTACACC



gi|156658313|gb|AM829237.1|AM829237 AM829237 DL, diurnal library Nicotiana tabacum cDNA clone nt005059071, mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||||||| .||||||
ACTGTCTTCTCTCTCTCGTAA

EST sequence (targeted fragment marked with yellow):
CATGCGGCCGCTGGACAACCTGCTGTAACAACGAGCAGTATTAACGAGAGTGCCATCTCATCTATGCATGATATGCGAGGATCAGATTTTGTTTCCCCTTCTTTGTTCCACACCAGCTCAACTGGATCTGAAGTCTTGCATGTTTTGGACACATCTTCTACTATTCATGGGCTATCAGGGATATCGAACTCCAGCAATGCTCTCTCTCTTCTGTCATCCCAATCACGGGGCTCGTCAAATCATTCCACTGTTTTTCGTGCCGCTCATCC



gi|12666197|gb|BE559491.2|BE559491 NtSUP pGNt Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
| |||||||||||.|||| |
AGTGTCTTCTCTATCTCGATC

EST sequence (targeted fragment marked with yellow):
CCAAGCCTTAGTTCCAAATCCAAATCCTCTTTCGATTCACTAAGCAGTCCAATTCCCAAGTCCAATCTCAAAAACTCAGACCTCTTCACCACTTCACAACACTCTTTTCCTTGCAAGAAAGAACCGATTTTCTCAAATTTGGCACTTCCCCTTTTTGTCAAATCTCCATTTCTTAGCTGACTCAAGTGATTTAATGGGACACCATCTTTTCCCCATTTTCTCATTTCAGAGCCACCAGATGTAGAAGAAGAAAAGGAAGGAGATAATAATGGAGGTAATGTAGAAACAGAAGGAGGAAATTTTCTTGAAGGAGAAGATGGTGGGGATGAAACAAAACTAGGGTTAAGGTTAGGGTTAGGGTTAGGGTTAGGTTCAACATTAAGGTTTAGAAGAGAATAGTGACTAGTAGTACCATTATCTTTTGGGGGCGATGACTGTAGTCTAAGTCTAGCTCTATCTCTTCTGTGAACATTCATGTG



gi|190876564|gb|FG177955.1|FG177955 AGN_RNC117xa08f1.ab1 AGN_RNC Nicotiana tabacum cDNA 5', mRNA sequence

TGACAGAAGAGATGGAGCATG
|.||||||||||.|||| ||
ATTGTCTTCTCTGCCTCTAAC

EST sequence (targeted fragment marked with yellow):
ACCTACTACTTTTCAACCCATTGGTCGTCAGCTCCTCTTGCCGACTCCTAATTTACCTGTGTTTCCTCAATCTCCGTCTCTTCTGTTAATTCGTTTCCTCTTCTTTGATTTTCTCGATTTCTTCTCCCCTTTCCAAAACCCTGTTCTCCATATTTCTCAATTCCAATTTTCTTCATTCAATTTATCAATTTTTTCGCACATAGATCAGCCATATTGTTTCTCTCTGAATTTCTTTTACCCTTTTACAAACAAAAATCCCTGAATTCCCAATTTCCTTATTTACTAAATTCGTCAAATAATAAGCACACAGATCAGCGATGATGATGCTAGTAAACTGTTCCAATTGTCATACACCATTACAGCTACCACCAGGTGCACGATCCATTCGCTGTGCGATTTGTCAGGCAGTAACACAGTTGGCTGATCCACGTACTGCTCCTCCGCCATCATCTAATCAATACAATCACCCACCACCTGCTTCCTCTCCCTCTCCCTACAACCACGCACCTCCCGGTCCCCCACCCAATGCTCATGGTAGAAAGAAAGCTGTGATTGTTGGTATATCCTATAGGTACTCTAGGCATGAGCTGAAAGGGTGTCTCAATGATGCTAAGTGCATGAGACATCTCTTGATGAACAAGTTCCATTTTCCTGAGTCCTCCATTCTAATGCTTACCGAAGAGGAGACCGACCCATACAGAACTCCGACTAAACAGAACATGCGCATGGCATTATATTGGCTTGTACAAGTTGCCACGGGAGACTCGCTGCTATTTCATTATTCTGGTCATGCTCAGCAAGATACAATG



gi|190734002|gb|FG195446.1|FG195446 AGN_PNL223ar1_h1.trimmed.seq AGN_PNL Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
|| ||||||||||||||| .
TCTTTCTTCTCTACCTCGTTT

EST sequence (targeted fragment marked with yellow):
CTTGAAACTAGGAACTTCATCAGTTCGCAATGTCATGGATATGAATGCATTTTTCGGAGGATTTGCTGCAGCGTTAGCATCAGATCCAATCTGGGTGATGAATGTTGTTCCAGCACATAAGCCTTTAACACTTGATGTGATTTTTGACAGAGGCCTTATTGGAGTTTATCATGACTGGTGTGAGCCTTTCTCAACATACCCTCGGACTTATGATCTGATTCACGTGGGAGCCATAGAATCTCTCATAAAGGATCCCGTTTCTGGAAAGACCAGGTGTAGCCTTGTGGATTTGATGGTAGAAATTGATAGAATGCTTCGGCCTGAAGGAACTGTTATAATTCGGGACTCTCCTGAAGTGATAGACAAAGTAGACCGGATTGCTCCTGCTGTAAGATGGACGGCAAGTATTCATGAGAAAGAACCTGAATCACATGGGAGGGAGAAAATCCTGGTAGCCACCAAGAATTTTTGGAAGCTACCTTCTGCATCCCACTGATTGAACCTTTTGTTTTTGTACTTTTAGGTATATCTCCTTTTTTCCTCGTTGTCTCATTACTATGGCCAATTGGCATGTTTCAAGTTATGCAAGTAGTCTACTTTTTGCTCCATCTCTTCTTTCTATCTAGTTTATGTAGAATTAATTGGAAGCGTACAAAGATGGTGGGAGAAAACTGTAAAAAAATTATAGCATACTGTTACAAACACTTCTATTAGA



gi|92038731|gb|EB450436.1|EB450436 KT7C.106L15F.051219T7 KT7 Nicotiana tabacum cDNA clone KT7C.106L15, mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||||||| .|||||
ACTGTCTTCTCTCTCTCGTTA

EST sequence (targeted fragment marked with yellow):
CACAAAAGAATGAAGACGTGGTCCTTAAACGTCACTCTCTAACTTTTCCTCTTCTTTTCTCACAAAATTCCCACTTCATATCATCATCTTCATCCTTTGTTCTTCTTCTTTTCTTCAATTCCTCTTCCACTTTAGTTTTTAATATGGAAACAGCTGGTCATCATAATATTTCTACTAGTGAAGATGATTATAAGAAGAAGAGAGGCAGTTGCAATAATAATATTACAACAAATAATAACCATATTAAGAAAGGGTCATTAAATAATGGAGGGTCAAGTACTACTCAAAGGTGTTGTCAAGCTGAGAAGTGCAGTGCTGATTTAAGTGATGCTAAGCAGTACCATAGGAGGCATAAAGTCTGTGAATACCATGCAAAGGCTCAAGTCGTCGTCGTCGCCGGATTCCGGCAACGCTTCTGTCAACAATGCAGCCGATTTCATGAGTTGACGGAATTTGATGAATCGAAAAGGAGTTGCCGAAGGCGTTTGGCTGGACACAACGAAAGGCGAAGAAAGAGCTCATCATCAACTGCAGAGTCACATAATATTGCAGAAAGTTCAAGCAGAAAAGGGACAGTGCAACTCAATAATAAGGACATTAACATGATATGTGGTCAAGTAAATGACAAGGGAAGAATTCAGATTTCAATTCAAGAAAATTCCACTTTCAAAAACTTCCACCTCCGTTGAAGAAATGCCTGCATATTAATTAATTGCTCTCTCTCTTCTGTCATTCTCTCCAAGTTCTATTCCTGAAATAATCATCACTCACACGGCCATTAGTACTACTGGCTTTATCTGCACTAGAAACAGACTATCAATTATTATGATGATGTATTAGATAGTACTATTAAGAAAAAGCCA



gi|190872059|gb|FG164766.1|FG164766 AGN_RNC012xi20r1.ab1 AGN_RNC Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||||||| .|||||.|
ACTGTCTTCTCTCTCTCGTGC

EST sequence (targeted fragment marked with yellow):
CTCAGCTGATCTATGCAAACTCTGATACTTTTACTGCAGCAGTCTTTCATTAGCAGTCACAAATTTTTCCAAGTGCAGTGTGAGCTCTGCATGGGCTGGCGTAGTCAAAACGGACAGCGACATGGTATTATACAACAATATAAATTGCATGGACAATCAAAACTCGTTCCTGATTCTTCGGCTCGTAGTAGCTACAAAGGAGGAAATCAATTCCAGTTCATGCAAGGCAGTGAGCGTAGTCTCCCTGAAGCTTCAATCTGCCAGCCACTTGTTGATCATCCCAATTCTACAGCAGCAGTTCCTAGCAGCGGACAAAAGATCTTCTCCAGTGGATTGAACGAAATTGTGGACTCTGATCGTGCTCTCTCTCTTCTGTCATCAGCACCCGCTGTAACTAGGGAGATTGGTTTTAGTCACATGGTGCAGCAGCCTGCCTCTATCCCCCGGCCCCGGCCCCAGTCACAATCTGTCGTACATAGCCTGCACTATGGTGGTCTAAGCCAGTACCCTTTTGCTCAGGATTTCAATAGCCAACCTCAAGATTCTGCTGCAGATTCACATGTTAGCAACAGCAGCTCTCTGCATTTCCATGATATGTTACAAAATGCACCAGATGGATCATCTACAAGTGGTGGCTGTCAGCAAACACTAGCCTTTATGTGGGACTAAATAAAATCAAAATTTGGTCTCTGTTAACATCAAGAAAATTCCTTCCAAGCAAATGTTATTCCGATTAAGAATGTTTAGCTTCTAATTAGCGACATAATTTCTTCTCTAAAATGTCGTAAGATCCTATCATGAAGTTAT



gi|156665506|gb|AM819197.1|AM819197 AM819197 COL, cold overnight library Nicotiana tabacum cDNA clone nt006165088, mRNA sequence

TGACAGAAGAGATGGAGCATG
||||| ||||||||| .||..
ACTGTGTTCTCTACCATGTGT

EST sequence (targeted fragment marked with yellow):
CATGCGGCCGCTAAATTGTGGAGCTGAAAGCGCTGATTGAAGGGCTTGATGTTGCTGTAATGCTGGGTTTGAAAAGGGTTAATGTTGTTTTGGATAGTCAAACTATCTTGCAATATGTTAGGGGCAAGTTGCATCCGAGGAATGGTAATGTTGCTGCACTTGTTGAACAAGCAACTTCTCGTCTAAGAAAATTTACAGATTGTGTACCATCTCTTGTGTCACAGAATACCGTCAATTTTGCGATTAAACTTGCTACTGATGCAATAGTCTCTCAGGTTAGAGGACCACCAGGAAGTAGTCTTCAAAAGAGCATCACGGAGACTTGTACTATTTGTCTGCAAGATACTGATGTAGATCATATGTTTCTAATTAATGGTTGCCTGCATTATTATTGCTTTTCTTGTATGAATAAACATGTGGAAGCTAAACTCCTTCAAGGAATGCTACCTGAATGCCCTCATGATAAATGCAAGTCTGTGTTGAAATTAGATAACTGCAAAAAGTTCTTGATACCCAA



gi|190137415|gb|FG635584.1|FG635584 TT-04_M11 Samsun trichome library Nicotiana tabacum cDNA, mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||||||| .||||| |
ACTGTCTTCTCTCTCTCGTTC

EST sequence (targeted fragment marked with yellow):
ATCACAATTTCCTCCATTAATTCTTCGTCTCTTTATCAACTTTTTAAATGGAAGCAGCTGATAATAATGACTTGTTCATTTTTGCCACTGATAATGACGACAAGAAGAAGAGAACCCCCAGTTATAATAATAGGAGTAGTAGTAGTAGTAATAATAACAATGAAAAGATCAAAGGGTCAAGTACTTCAATGAGGTGTTGCCAAGCTGATAAGTGTACTGTTGATTTAAGTGACGCCAAACAATACCATAAGAGGCATAAAGTCTGTGAATACCATGCAAAGGCTCAAGCCGTCGTCGTCGCCGGACTCCGGCAACGCTTTTGTCAACAATGCAGCCGATTTCACGAGGTAGGAGAGTTTGATGAATCGAAAAGGAGTTGCAGAAGGCGTTTGGCTGGGCACAACGAAAGGCGAAGGAAGACTACTACTACTTCTTCATCATCAGATCAGTCTAATGCTGAAGTTTCATCGAGTAGAAAAGCCATAATAGGGACAGACACTCATCAGATAATAAACTTCCAAGAAAATGCAGCAGCAGGTTATAAACACTTTCAGATTGGATGAAAAACTGAGGCAAAAAAATGGATACGATATGCTTGCTCTCTCTCTTCTGTCATTCTCTAATAAGTTCAGTTACTATAATAATTATCAGTTCGCTGTTTGTGACTTTTATAATCATGAAGTTTGAAGTCTTGAACATATTATTAAATCCAAGATCCTCAGTGGTAGTTTTGTACCACTTTATCAAAAATAAATAATGATCTATGGATGTTATTGTATAATTAAACAGCCTCTCTATCTTC



gi|254636838|gb|FS434292.1|FS434292 FS434292 normalized full-length tobacco cDNA library Nicotiana tabacum cDNA clone TBK02GR009

TGACAGAAGAGATGGAGCATG
||| ||||||||.|||| |..
ACTTTCTTCTCTGCCTCTTGT

EST sequence (targeted fragment marked with yellow):
GGCAAAAACCCATTTTCCCCTTTAGTTTCTCCAACATAATTGAGGTTCAATTCTAAAGCCAAAGAAATGGGTCGTCTAAATCTTCTTATAGCAGTAGTGTTATCAATATATTCAGTTGGTGTAGTGTTCATGCCCAACTTGGCTTTTGCACAACTGAAGACCAATTATTACGCCAAAACCTGTCCTAATGTCGAATCCATTGTTAGAAATGTGGTTAACCAGAAATTCCAACAAACATTTGTAGCAATCCCTGCTGTTCTCCGTCTCTTCTTTCATGATTGCTTTGTTGAGGGTTGTGATGCTTCAGTGATAGTATCGTCNACACCAGGGGAACACAGCTGAGAAGGATCACCCAGATTATCTATCATTG



gi|254643824|gb|FS392651.1|FS392651 FS392651 normalized full-length tobacco cDNA library Nicotiana tabacum cDNA clone TBK01A01NG

TGACAGAAGAGATGGAGCATG
|||||||||||||.||| ||.
ACTGTCTTCTCTATCTC-TAT

EST sequence (targeted fragment marked with yellow):
GGTAGCTCAGTCTATAGCAGCCAGCCTATAGGAACTTATCTCTATCTCTTCTGTCAGTTTACTACTTCAAGTCAACTGGATTTAGATTCAGAATTAGATACGTTCGAAACTGGAACACTTCCCCTAACTCAACTGCTTGAACTCATGAACAACTCTTTGCAGTTCGCTCGTTTCTTCTTCCTCTTCATCATAACTTTCGTGCGCGAATCGTCCAGCTCAGCGATCATCAATCCATCCAAAGCCAAGCAAATTTCATGGAAACCTAGAGCATTTGTATATGAAGGATTTCTTACGGATGAAGAATGCAATCACTTGATATCTCTCGCAAAATCGGAGCTGAAGAGATCGGCTGTGGCAGATAATGAGTCTGGAAATAGTAAGACCAGTGAGGTTAGGACTAGCTCCGGAATGTTCATTCCCAAAGCTAAGGTACTCAATCCGCGTGTTCGGAATTCACAATCCACTGCCTTTGATCCACTTGGCAATACCCGCCCCATCCCTGACACAGTTGTTTTAATCTAATAATTTGATGATTGCTCCCTTTAATAATAGCTGATGACTTCTTACAGGCTTCAGAT



gi|156644086|gb|AM783587.1|AM783587 AM783587 COL, cold overnight library Nicotiana tabacum cDNA clone nt006014010, mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||||||| .|||||.|
ACTGTCTTCTCTCTCTCGTGC

EST sequence (targeted fragment marked with yellow):
AATGGCCTCCTTCTCCAATCTAAAACTACTGCAGATTCTACCCGGGGAACCTCTGAAGTTCCCAAGTTCACAATAACAAGAGGGTTAACTTCAAAGCCTTACAAAGCTGGCAGTATTAATGTGCGGTCACTTGTGGGGGAAATCAAGCTTCCAGGGGCAACTAAAATGCAGACTGATGCATCCAAGGGCACCACCACCGAGGTCTTCAGCCAAGGTGTGAAGGAGAACATGTTGTCTCTCAATATGGGTGCGGAACCAGAATTACCTCGTGCTCTCTCTCTTCTGTCAAATAAATCTTCATCCGAGCCTGAATCTACTTCAATTGGCCACCCCACACATGTAAATTCAATTGGCCACCCCACACATGTAAACCAGATCAGCATAACGGAGCAGCAGGTGATTCTTGAGAATTCGCCACTTGGGTCCACTGAACACTGGCAGGCCGAGCAATATCTACTCAATCCACGCATCCATGCTTTGGTTGCTAATGGTCATAGTGGTGGT



gi|190738865|gb|FG150403.1|FG150403 AGN_RNC112xa07f1.ab1 AGN_RNC Nicotiana tabacum cDNA 5', mRNA sequence

TGACAGAAGAGATGGAGCATG
|.||||||||||.|||| ||
ATTGTCTTCTCTGCCTCTAAC

EST sequence (targeted fragment marked with yellow):
CCTAATTTACCTGTGTTTCCTCAATCTCCGTCTCTTCTGTTAATTCGTTTCCTCTTCTTTGATTTTCTCGATTTCTTCTCCCCTTTCCAAAACCCTGTTCTCCATATTTCTCAATTCCAATTTTCTTCATTCAATTTATCAATTTTTTCGCACATAGATCAGCCATATTGTTTCTCTCTGAATTTCTTTTACCCTTTTACAAACAAAAATCCCTGAATTCCCAATTTCCTTATTTACTAAATTCGTCAAATAATAAGCACACAGATCAGCGATGATGATGCTAGTAAACTGTTCCAATTGTCATACACCATTACAGCTACCACCAGGTGCACGATCCATTCGCTGTGCGATTTGTCAGGCAGTAACACAGTTGGCTGATCCACGTACTGCTCCTCCGCCATCATCTAATCAATACAATCACCCACCACCTGCTTCCTCTCCCTCTCCCTACAACCACGCACCTCCCGGTCCCCCACCCAATGCTCATGGTAGAAAGAAAGCTGTGATTGTTGGTATATCCTATAGGTACTCTAGGCATGAGCTGAAAGGGTGTCTCAATGATGCTAAGTGCATGAGACATCTCTTGATGAACAAGTTCCATTTTCCTGAGTCCTCCATTCTAATGCTTACCGAAGAGGAGACCGACCCATACAGAACTCCGACTAAACAGAACATGCGCATGGCATTATATTGGCTTGTACAAGTTGCCAACCGGGAGACTCGCTGCTATTTCATATCTGGTCATGCTCAGCAAAGATACATGAGATGA



gi|83423260|gb|DW005254.1|DW005254 KL4B.100C04F.050922T7 KL4B Nicotiana tabacum cDNA clone KL4B.100C04, mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||||||| .|||| |
ACTGTCTTCTCTCTCTCGCTC

EST sequence (targeted fragment marked with yellow):
GATCATCACAATTTCCTCCATTAATTCTTCGTCTCTTTATCAACTTTTTAAATGGAAGCAGCTGATAATAATGACTTGTTCATTTTTGCCACTGATAATGATGACAAGAAGAAGAGAACCCCCAGTTATAATAATAGGAGTAGTAGTAGTAGTAATAATAACAATGAAAAGATCAAAGGGTCAAGTACTTCAATGAGGTGTTGCCAAGCTGATAAGTGTACTGTTGATTTAAGTGATGCCAAACAATACCATAAGAGGCATAAAGTCTGTGAATACCATGCAAAGGCTCAAGCCGTCGTCGTCGCCGGACTCCGGCAACGCTTTTGTCAACAATGCAGCCGATTTCACGAGGTAGGAGAGTTTGATGAATCGAAAAGGAGTTGCAGAAGGCGTTTGGCTGGGCACAACGAAAGGCGAAGGAAGACTACTACTACTTCTTCATCATCAGATCAGTCTAATGCTGAAGTTTCATCGAGTAGAAAAGCCATAATAGGGACAGACACTCATCAGATAATAAACTTCCAAGAAAATGCAGCAGCAGGTTATAAACACTTTCAGATTGGATGAAAAACTGAGGCAAAAAAATGGATACGATATGCTCGCTCTCTCTCTTCTGTCATTCTCTAATAAGTTCAGTTACTATAATAATTATCAGTTCGCTGTTTGTGACTTTTATAATCATGAAGTTTGAAGTCTTGAACATATTATTAAATCCAAGATCCTCAATGGTAGTTTTGTACCACTTTATCAAAAATAAATAATGATCTATGGATGTTATTGTATAATTAAACAGCCTCTCTATCTTCATAAAGTGGGGCCAGATTTCGCTATGTGGGATTACGCTTGAGTATGTTGTTGTTGGATATTGTTGTAATTGCCCCCACTACATATATATGATCTGCTTATAATTCTACAAAAAAA



gi|156662670|gb|AM798619.1|AM798619 AM798619 COL, cold overnight library Nicotiana tabacum cDNA clone nt006198070, mRNA sequence

TGACAGAAGAG-ATGGAGCATG
|||||| |||| |||.||||||
ACTGTCCTCTCCTACTTCGTAC

EST sequence (targeted fragment marked with yellow):
TGCATACTCCACCAGACCACTATACCACATACCCCCCCAACTGAATGGAGTTGTTGAAAGAAAACATAAACATCTCTTAGAAACTTCTAGAGATCTAATGTTCCAGTCCAACCTTCCTACTAAATATTGGGGTGATTGTATCCTCACTGCTACCTACCTAATTAATGGACTTCCTTCTTCTGTTTTAGGCAATTCATATCCTTTTGAAAAATTGCATGGTTCACCACCATCTTATGATCATTTGAGATCTTTTGGATGTCTTTGTTTTACAACTACACCAAAACCCGATAGAGATACATTTCAATCCAGGGCAATTACATCTATTTTTTTAGGATATCCATGTGGTAAGAGAAAGTTACAAACTACTTAACATCTCCAACCATTCTATTTTCTTTTCAAGGGATGTAAAATTTCATGAATCTATTTTCCCTATTCTTCCTCCCTTCCTTCCTCTATTTTCCCTGTGTCTTTTCCTGACTCTACAGATATTCATGCTTCATCCTCTCCTGTCATTTTTCCTTCTCCCACACCTGGTTTCACATCTGAACCCACTCCTTCCACTCCTCCTACTATCCCTCCTACCTCTC



gi|52831479|gb|BP527752.1|BP527752 BP527752 MAT001 Nicotiana tabacum cDNA clone BY12562, mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||||||| .|||||..
ACTGTCTTCTCTCTCTCGTGT

EST sequence (targeted fragment marked with yellow):
CAAGAAGCAAAAATAATGTGAGTAGCAACTCATATCTAAATGATATTGAAGGATCAGATTTTGTTTCCCGTTCTCTGTTCCACAGCACCTCAATTGGGAGCGAAGTCTTGAATGTTATGGATTTGGCCTCAACTTTTCAAGGGATATCAAGTTCTGGCTGTGCTCTCTCTCTTCTGTCATCTCAATCACAAGGCTCGTCAAATTACTCATCTGTAGTTCCTAATTCTCACCACCTTATTGCACCTAGCGATAATGCCCATTACAATGTGACACAGACATCTGAAAAGTTCCCAGGGATGAGCCAGAAGGATTCAACAAGTAATGCGTCCGGTATATTCAATATATCCGGAGCAATTTCTGCAGAGGGTCGCTTGGAACAGATACTAAGAAACAACTGTGGCATCAACGGGAGTATTCA



gi|190861693|gb|FG151964.1|FG151964 AGN_RNC109xn13r1.ab1 AGN_RNC Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
|||||||||||| .|||||.|
ACTGTCTTCTCTCTCTCGTGC

EST sequence (targeted fragment marked with yellow):
TCCAAAGCCTTGAAAGAGCTGCAGTACAAATGGCTGTCATTATTGCCAGGGATCCAGTTGTCACGGGCCGTGGGTGCACACAGTAATGTATCTAGCTGCTTGCTCTTGCCCTCCAAGGGCCACACATCCGAGGTTTTCAATCGAGGTGCCACGGAATCCATGTTCAATATGGGTACAGGACGGGAATTTCCTCGTGCTCTCTCTCTTCTGTCAACTAATTCATGGGGTTCATCCGAGCCTGAGACTGTTTCACTGAACAACCCAACACACGCGAATCAGAGCAGCATGCCCGAGCAGATGATGCAGGCAATTCCACAAGGTGTGCCACTTATGTCCTGTGAATACTGGCAGGGTGAACAACCCTCATCCGATCCTCGTGACCATACATTGGCTGCTAATAGTCATGCTGGTGGTAGCTTTCAGGGAATCGAGCTGTTCAAACCTCCATTTGACACTGACTTTTATCTCAATGCATTGAACTGAAGCCAACATTTGATGCCTTTAGTGGATTTTGGCCAGCTCCTGTTTTCGCTTTTTGACTCGAACTTGGATCAAACAGGAGGTTTATTAGTAGGCTATTTGAAGCAGTTTGTTTGCCATTTTAAATTAGTACTGGTATATATAATATATATATATATATATATATATATAGGTTGGGCTTTGAAACTCCAAGAAAGTTTGCTTGATTTAGGCAGTGACAAGGGTTTTTAAGAATTGTAGTTTCTTTATTTTGCAACAACTGCACAGGCCCCGAATGATTGTTTTTCCTTTTTCACCATTT



gi|190734040|gb|FG198344.1|FG198344 AGN_PNL227ar1_f9.trimmed.seq AGN_PNL Nicotiana tabacum cDNA 3', mRNA sequence

TGACAGAAGAGATGGAGCATG
.|| ||||||||||||||| .
GCTTTCTTCTCTACCTCGTTT

EST sequence (targeted fragment marked with yellow):
ATTCATGTGGGAGCCATAGAATCTCTTATAAAGGATCCTCTTTCTGGAAAGACCAGGTGTAGCCTTGTGGATTTGATGGTAGAAATTGATAGAATGCTTCGGCCTGAAGGAACTGTTATAGTTCGGGACTCTCCTGAAGTGATAGACAAAGTAGAACGAATTGCTCCTGCTGTAAGATGGACGGCAAGTATTCATGAGAAAGAACCTGAATCACATGGGAGGGAGAAAATCCTGGTAGCCACCAAGAATTTTTGGAAACTACCTTCTGCATCCCACTGATTGATCCTTTTGTTTTTGTACTTTTAGGTATATCTCCTTTTTTCCCTTATTGTCTCATTACTATGGCCAATTGGCATGTTTCAAGTTATGCAAGTAGTCTACTTTTTGCTCCATCTCTTCTTTCGATCTAGCTTATGTAGAATTAATTGGAAGCGTACAAAGATGGGAGAAGCCTCTCTTCTTTTCTCTTCCCTCTTTCTTCCTTTCTTGCTATCCACTTCTTATTATTACCTTCCTGAGCCGAGGGTCTATTGGAAACAAGCCTCTCTACCTTTAAGGTAGGGTAAGGTTTGCGTACAGATTACCCTCCCCAGACCCCACCTGGTGGGAACATACTGGGTTTGTTGTTGTTGTACAAAGATGGGAGAAAACTGTAAAAAAATTTATAGCATACTGTTACGAGCACTTATATTAGA