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mature miRNA: TCTTTCCCATTCCTCCCATCC
species: Nuphar advena
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|31393832|gb|CD472564.1|CD472564 nad01-1ms6-d07 Nad01 Nuphar advena cDNA clone nad01-1ms6-d07 5', mRNA sequence

TCTTTCCCATTCCTCCCATCC
|||||||||||| |||| ||
AGAAAGGGTAAGTAGGGGAGC

EST sequence (targeted fragment marked with yellow):
GCCGAATTCGGCACGAGGCTTACCCTCCGTTTCTTTATCTTTTTCGTTTTCTTACTCCGCCGTTAGGGTTAGGGCTTTTGTGTCGTATCAGCACATCATCCGACCCTCTCTCGATGGCTTCTGCGGATGTGGTCGAATACCGTTGCTTTGTAGGTGGCCTTGCTTGGGTCACGGATGATAGGAGCCTCGAGGAGCGCCTTCAGTTCCTTTGGGGAGGTCATCGAAACCAACGTTTGGTTACTTCCTTTCATCTCCTGATTCCATACGAGGACCTTTCTCAACCCTCTTTTGACTGATCCGTGGTTATAGTCATCCTTGTCTTTTGGTTTGCAGGTCATCAGCGACCGTGAGACGGGTAGATCCAGGTGCTTTGGTTTCGTCACCTTCAGCGATGAACAATCGATGCGCGATGCCATCGAGGGGATGAATGGGAAAGAGCTTCACGGTAAGAAACATAACCGTTAATGAAGCCCAGACCCGTGGCGGTGGTGGATTTCGCAGTGGTGGTGGTGGC



gi|164500560|gb|FD382801.1|FD382801 Nad03b_84_C02_C005.g1 Nuphar advena flower bud library (Wash U) Nuphar advena cDNA clone 122

TCTTTCCCATTCCTCCCATCC
||.||||||||| |||| ||
AGGAAGGGTAAGTAGGGGAGC

EST sequence (targeted fragment marked with yellow):
ATCCCAGACCCTCTCTCGATGGCTTCTGCGGATGAGGTCGAATACCCTTGCTTTGTAGGTGGCCTTGCTTGGGTCACGGATGAAAGGAGCCTCGAGGAGGCCTTCAGTTCTTTTGGGGAGGTCATCGAATCCTAGGTTATCATCGACCGTGAGACGGGTAGATCCAGGGGATTCGGTTTCGTCACCTTCCGCAATGAACAATCGATGCGCGATGCCATCGAGGGGATGAATGGGAAGGAGCTTGACGGAAGAAACATAACCGTTAATGAAGCCCAGACCCGTGGCAGTGGAGGGTTTCGCAGCGGCGGCGGCGGCGGCTACGGCAGGAGAGTTGGTGGTGGAGGCTATGGCGGTGGAGGCGGCCGTCGCAATGATGGTGGGGGTTACAACAACCGTGGGGATGGCAGCCGCTATGGTGGCTCTGATGGTGGATTTTCGAGGGGTGGTGGCGCTTCCGATGGTCACTGGAGGAACTAGGGTTTGCTACTTATTTTGTTAGGTTAGGATGTCTGTCCGCGTCAATCAGTCTGTCCGTCCGTCTGCCGTTCCTTTGATCGTTATGTATTAGAGTGATGCTTTTCTGGTCTTTGATCATCGTCTTGTTATTTGCATGCGTGAACTTTT