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mature miRNA: TCAGATGCTTTAAATTCCCGA
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|292514363|gb|GW733840.1|GW733840 WS0495.CR_D03 WS-CBM-34 Picea glauca cDNA clone WS0495_D03 3', mRNA sequence

TCAGATGCTTTAAATTCCCGA
|||.||||||||||| |||||
AGTTTACGAAATTTA-GGGCT

EST sequence (targeted fragment marked with yellow):
GGGGCTCGTAAAAATGGCGTGAGCCTGAACAGCAGAGGCAGCGTATATACGCCAACAGATCAGACCACTTCAGGAGCCGAGCAGAAACGACTGGCTTAAATCCAAGAACTTTAAATGCCTTAAATTCCCGATAAATTCAGGCGATGACCGAATATATTTCGGGATTTAAAGCATTTGAAGTTCTGGATTTATCCCAAACAGGAGCGCAAACATGCGATTATATGCCATCCACGGTAATACATGCAAAGAGAAGGAGAGCATCGACGGATGACAGGGGAAGGTCCAGGGCCCCGGCCTTAGAAAGGAGCTGCTCATTCGACCGATCTTTGATCCGAACGCTGTGACGGAGGGCTTTTCGGGCGGAGGGAGGGCTTTACGGAACAGATAACGCCGGCCCACAACGGCAGACGGACCGCCCGACGGCCAGCAATTTCAGACGGCAGACGTTTTTCTGGCGAGGATCTCTTCAGCCGCCTGCCTCCGCCGTTGACGGTAAACCGAGATCTCGATATTACCGGCCAAAGCTGCCTTCAATGAACATGGAACGGGACGGCAGTCACGGCACGGATCTGGGCGTAAATTTTAATTGATTTAAATTTTTTTACCAGGTCACATTATTCTCATTTTGCACAATTTTCATGTCATTTTTTAATGCATATATTTTGTTCGGGAGTTGATTGAAAAAAAAAAAAAAAA