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mature miRNA: TGGATTGGAGTGGAGTGGATT
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|238891761|gb|GR223253.1|GR223253 GQ04006.SP6.r_L12 GQ040 - Shoot tip - Active growth (Normalized) Picea glauca cDNA clone GQ0 

TGGATTGGAGTGGAGTGGATT
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ACCTAACCTCATCTCGCGTTA

EST sequence (targeted fragment marked with yellow):
TAGATATCATAGAGTACCGACCACACGGCCGGTTGCTGAAATACGAGGCCTCCACCAAGACAACCACCGTGCTTCTCGAAGATCTTTACTTCCCCAACGGAGTGGCTCTCTCTGCCAAACAGGACTTCCTCGTCTTCTGTGAAACTTCAGTGCATCGCTGCCAGAAGTACTGGTTAGAAGGGCCAGACAAAGGCACCGTCGAAGCTTTCATTGATAATCTCCCTGGACTTCCAGATAACATCCGCTATGATGGCAACGGCACCTTTTGGATCGCACTTCCAACGAGCCGAACCTTGTCCTGGACAATTGCAACCAAATTCCCTTCTGTGAGGCACGTGTTGGTTCTGATAGGGAAATTGGCGCCAAGTGTTGTCATCGATGCTATAGTTAATGAACCAAGTGTTCTGGCAGTGAATGAGATGGGCAATCCGATTGCGCTCTACTCCAATCCAAATTTGAAGAATCAAATTACTACAGGATTGAAGATAGGAGATTCTCTGTATTATGGATCTGTAACTCTAGATTATATTGGACGTGTTCCTCTCCCCCAACAAATACCAATAAATTGAAAATGGATAGAAAGTCAGTTCTACCATTTCAGAACTGAATAGGGATCCGCTGGTCCGTACTTTTGTCAGAAATGAGTTGGGATTTGTTTGTCCACTTATTTTCAAATTTCAATGCTGATAAATTGCAAAACCAGGTTGTTAAAAAA


gi|238891002|gb|GR222415.1|GR222415 GQ01308.T24.r_C21 GQ013 - Elongating ROOTS tips - sapplings Picea glauca cDNA clone GQ01308_ 

TGGATTGGAGTGGAGTGGATT
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ACCTAACCGCATTTCATCTTA

EST sequence (targeted fragment marked with yellow):
ACGGAGAACACATCCACACCGCCATCATCTCCCCCGGAAAAGCTTACGGACTTGGCACGGGAGCAGGGTAAACACAGAGCTACACCATTCCCTTTTTTGTAGGAGACATACCGAATTTTTGGAACCGACCGTTTGTACTCGGGGAGGGAGAGAACATGTAGTGGTATGGTCGTATATCGGAGATGTGACTGGGCTTTTTCTACGTTTAGTTGAGTAGGCAGCTACCGACGGCGCAGGCGCAGAGTGGGGTGAAAATGGATTCTACTTTACGCCAATCCACGACGACGGCATCCCATGGAAAAGCATTACCAAAGCGACGGGGAAGATCTTGAAAGAACTGGGGGTGTTTGAAGATGAAACTCCGGTCTCAGCCACATCAGCAGTCACAGATAAGATGCTGGATGGCTCGGGACAATTGGGACTACTTATGTATGGCAGCAATTATAGAATTCGTGCGGATAGAGCAAGCAAGTTGTTTAGGCTTGAGGCGGAGGGGCCGTGGGTTTTGGAGGTGTTAAAGGATGATTTGGGAGCTGCCTGTGAAGGATAAGCAAAGGGATGTTGAGACTTTGGTGAATCTTCTAAAGGGGTGAAGGTGCTGCTGGGTAATGCACTCTCACTCGTTA