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mature miRNA: TTATTAAACCCGAACCGGTCT
species: Populus tremula
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|38578216|gb|CK093891.1|CK093891 I003P45.3pR Populus senescing leaves cDNA library Populus tremula cDNA clone I003P45 3', mRNA 

TTATTAAACCCGAACCGGTCT
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AATAATTTGGGCTTTCCCATA

EST sequence (targeted fragment marked with yellow):
GTCCTTTTAAAAAGAAATAGAATTCTAGTTCTTGATGAAGCTACTGCTTCCATTGATTCTGCTACTGATGCTGTTCTACAAAGAATTATCAGGCGAGAATTCTCAGATTGCACAGTGATCACAGTAGCTCATAGAGTTCCGACTGTTATAGATAGTGACATGGTTATGGTTCTCTCTTACGGGAAATTGCTGGAGTATGATGAACCGACGAAGCTACTGGAAACGAGCTCCTCCTTCTCTAAACTTGTAGCTGAATACTGGGCTAGTTGCAGGCAGCACTCTCGCCGGAATTTTGAAAAACAACCGGTCATGATTTCATGATGAGAATCACAAGAGCTTTTCCTCTCCAGAAAATCACATACCCTTTCGGGTTTAATAAAAGCTTACTCGGAAAAATT