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mature miRNA: CTTTGTATTCTTCCACAGCTT
species: Populus tremula x Populus tremuloides
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|23999385|gb|BU826595.1|BU826595 UK122TE05 Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA 5 prim 

CTTTGTATTCTTCCACAGCTT
 ||||||.||||||| |||||
TAAACATGAGAAGGT-TCGAA

EST sequence (targeted fragment marked with yellow):
CTCCCTGTGAGTGGGGAAGATGTATGCATTAAATTAAGAGAACAGGATCAATCTTGGGGAGATTTTCTTGCCAGTGTCAAACACCTTCCAAGGTCAAATCATTACTCTCCAGTTTATAATCCAATAGACTTTCCGACAAACATACTCTTTTCATCTGGAACCACAGGAGAACCAAAAGCTATACAATGGACACAGCTTTCGCCAATTCGAGCAGCTAACGACGGATGGGCTTATGATGATCTTCAAGTTGGAGATGTTTTCTGCTATCCTACAAATCTAGGATGGGTAGCAGGACCAATTGTGCTTTATGAAGCTTTTCTAAGCGGTGCAACTTTTGCTCTCTATCATGGATCTCCTCTTGGCGGTGGTTTCGGAAAATTTGTTCAGGATGCCGGTGTGACTATTCTGGGCACGATCCCATCTATGGTAAAAGCTTGGAAGAGTACAAATTGCATGGAAGGCTTAAA


gi|23998191|gb|BU826027.1|BU826027 UK115TH01 Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA 5 prim 

CTTTGTATTCTTCCACAGCTT
||||||||||||  |||| ||
GAAACATAAGAACTTGTCTAA

EST sequence (targeted fragment marked with yellow):
GGTACCCACTTCAACAAGAAGAAAGTCGAAACTCCCTCTCAACCACCCAACAATGGCTTCTACGGTTTCGTTCTGGTCTTGTATATTCCTCCTCTCTCTAATTGTAGCGCTCTCTGCTGGAGAGGATGAATCAAAGGAGTACGTGTTGACTCTAGATCACTCCAATTTCAATGAGACCGTTAGCAAACACGATTTCATCGTCGTCGAATTCTACGCTCCTTGGTGTGGCCACTGTAAGAAGCTAGCTCCAGAGTATGAGAAAGCTGCTTCTATATTGAGCAGCAATGATCCTCAAGTTGTTCTTGCCAAAGTTGATGCGAATGAGGATGCTAACAAAGAGATCGCTAGTCAATATGACGTCAAGGGATTCCCCACCATTGTAATTTTGAGAAAGGGAGGGAAATCTGTTCAAGAATACAAAGGCCCTCTGAGGCAGATGGAATTGTGAGTATCTGAGAAACAAGTGGTCCGCTCTGCTGAATAAATCACGATGATGCACTGGTTATGG


gi|24010400|gb|BU831744.1|BU831744 T025C01 Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, 

CTTTGTATTCTTCCACAGCTT
||||||||||||  |||| ||
GAAACATAAGAATTTGTCTAA

EST sequence (targeted fragment marked with yellow):
CAGCAATGATCCTAACGTTGTTCTTGCCAAAGTTGATGCGAATGATGATGCTAACAAAGAGATCGCTAGTCAATATGACGCCAAGGGATTGCTCACCATTGTAATTCTGAGAAAGGGAGGGAAATCTGTTTAAGAATACAAAGGCCCTCGTGAGGCAGATGGAATTGTTGAGTATGTGAACAAACAAAGTGGTCCTGCTTCTGCTGAATTAAA


gi|60710541|gb|DN500351.1|DN500351 UK122TE05.5pR Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA cl 

CTTTGTATTCTTCCACAGCTT
 ||||||.||||||| |||||
TAAACATGAGAAGGT-TCGAA

EST sequence (targeted fragment marked with yellow):
CTCCCTGTGAGTGGGGAAGATGTATGCATTAAATTAAGAGAACAGGATCAATCTTGGGGAGATTTTCTTGCCAGTGTCAAACACCTTCCAAGGTCAAATCATTACTCTCCAGTTTATAATCCAATAGACTTTCCGACAAACATACTCTTTTCATCTGGAACCACAGGAGAACCAAAAGCTATACAATGGACACAGCTTTCGCCAATTCGAGCAGCTAACGACGGATGGGCTTATGATGATCTTCAAGTTGGAGATGTTTTCTGCTATCCTACAAATCTAGGATGGGTAGCAGGACCAATTGTGCTTTATGAAGCTTTTCTAAGCGGTGCAACTTTTGCTCTCTATCATGGATCTCCTCTTGGCGGTGGTTTCGGAAAATTTGTTCAGGATGCCGGTGTGACTATTCTGGGCACGATCCCATCTATGGTAAAAGCTTGGAAGAGTACAAATTGCATGGAAGGCTTAAATTGGACAAAGATAAGATCCTTTGTTACTGCTGGAGAAGCCTCTGATGTTGATGATGGGCTTTGGCTTTCTTCAAGAGCTTATTACAAGCCCATTATTGAAGTTTGTGGAGGCACGGAGCTTGCATCAAGCTACCTCCAAGGAACTTTATTGCAGCCACAAGCCTTTGGAGCGTTAAGCACTGCCTCAATGGCTACAGGCGTTGTCATCCTTAACGACAACGGAGTTCCTTATCCAGATGATCAACCATGTGTTGGTGAAGTAGGCTTGTTTCCTTTAATCATGGGAGCATCAGATAGGATGGCTTAATGCTGATCATGACAAAATT


gi|38586837|gb|CK102512.1|CK102512 G080P80.5pR Populus tension wood cDNA library Populus tremula x Populus tremuloides cDNA clon 

CTTTGTATTCTTCCACAGCTT
||||||||||||  |||| ||
GAAACATAAGAACTTGTCTAA

EST sequence (targeted fragment marked with yellow):
ACTCGATACGATGTGGCTTCTGAGCGTGCGTTCTGTTCTGGTCTTGTATATTCCTCCTCTCTCTAATTGTAGCGCTCTCTGCTGGAGAGGATGAATCAAAGGAGTACGTGTTGACTCTAGATCACTCCAGATTTCAATGAGACCGTTAGACAACACGATTTCATCGTCGTCGAATTCTACGCTCCTTGGTGTGGCCACTGTAAGAAGCTAGCTCCAGAGTATGAGAAAGCTGCTTCTATATTGAGCAGCAATGATCCTCAAGTTGTTCTTGCCAAAGTTGATGCGAATGAGGATGCTAACAAAGAGATCGCTAGTCAATATGACGTCAAGGGATTCCCCACCATTGTAATTTTGAGAAAGGGAGGGAAATCTGTTCAAGAATACAAAGGCCCTCGTGAGGCAGATGGAATTGTTGAGTATCTGAAGAAACAAAGTGGTCCTGCTTCTGCTGAATTAAAATCAGCTGATGATG


gi|24019676|gb|BU836864.1|BU836864 T091G12 Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, 

CTTTGTATTCTTCCACAGCTT
||||||||||||  |||| ||
GAAACATAAGAACTTGTCTAA

EST sequence (targeted fragment marked with yellow):
CCCAACAATGGCTTCTACGGTTTCGTTCTGGTCTTGTATATTCCTCCTCTCTCTAATTGTAGCGCTCTCTGCTGGAGAGGATGAATCAAAGGAGTACGTGTTGACTCTAGATCACTCCAATTCCAATGAGACCGTTAGCAAACACGATTTCATCGTCGTCGAATTCTACGCTCCTTGGTGTGGCCACTGTAAGAAGCTAGCTCCAGAGTATGAGAAAGCTGCTTCTATATTGAGCAGCAATGATCCTCAAGTTGTTCTTGCCAAAGTTGATGCGAATGAGGATGCTAACAAAGAGATCGCTAGTCAATATGACGTCAAGGGATTCCCCACCATTGTAATTTTGAGAAAGGGAGGGAAATCTGTTCAAGAATACAAAGGCCCTCGTGTGGCAGATGGAATTGTTGAGTATCTGAAGAAACAAAGTGGTCCTGCTTCTGCTGAATTAAAATCAGCTGATGATGCTACTGGTTTCATTGGTGACA