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mature miRNA: TCGCTTGGTGCAGGTCGGGAA
species: Populus tremula x Populus tremuloides
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|18014178|gb|BI130207.1|BI130207 G102P18Y Populus cambium cDNA library Populus tremula x Populus tremuloides cDNA, mRNA sequen 

TCGCTTGGTGCAGGTCGGGAA
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AGCGAACCGCGTCGAACCCGT

EST sequence (targeted fragment marked with yellow):
CTATTACTAGTTGAATTGAGCCGCGGTTCCTATATCTCTCTCTCTTCGGCAGATCAGATCCAGATCCCTCAAAAGAATATGGGTCGTGGAGTTAGCGCTGGTGGAGGGCAGAGTTCATTGGGCTATCTGTTTGGGAGTGGAGAGGCTCCAAAACCTAGCGCAAAGGGATGCCCAAGCTGCGCCAAGCGAGGTCCAGCCTGCAAACAATCCACCTCCTTCTAAACCTGCTGCAGCCCCTCAGCCAGCTGAAATTAACAGGGAGTTTCCTGCTGGGTATCAACAGTACTTCCACAAACAACTATTTGAGGGCAGATGGGTCAGAACGGCTGGCAACTTCATCACGGATCGACCT