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mature miRNA: TGAAGCTGCCAGCATGATCTG
species: Populus tremula x Populus tremuloides
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|18015013|gb|BI131042.1|BI131042 G114P38Y Populus cambium cDNA library Populus tremula x Populus tremuloides cDNA, mRNA sequen 

TGAAGCTGCCAGCATGATCTG
||| ||||||| ||.| ||||
ACTACGACGGTAGTGCAAGAC

EST sequence (targeted fragment marked with yellow):
GAATCGGATACACGAATTGGTGAAATTTCCATGCCATCCTTGGATGACTCAGTTGGGTCCCGTTCACATCCCAAAAAGATGTTTGATGTTGTTTATAGAAGGTCAAAACCAGGTAGAGGTAAAATTAACTCAGAACGTGATGGCAGCATCAGAGAAGAAACTGCAATTGCTTGTAACCCTCATCTTGACTCCAGAGGGGATTCCTATGAAGGCACAATTGGTGGATCTCATAGAATACATTCCATGGGCTCAAAGGGAACACATGATGCAAATATTGCAATCAATGATCTCCATTTGGGGCAGGGACATGAATCAGATGATACTTGTAGAGATACCCACAATGGTTCTGTTAGTAGATTTCAGCTTCCATGCGAAGGAATGGGGATCAAGTTCAAGGATGACAGTTCGATCGAGATCT


gi|14489534|gb|BI068914.1|BI068914 C029P19U Populus strain T89 leaves Populus tremula x Populus tremuloides cDNA, mRNA sequence 

TGAAGCTGCCAGCATGATCTG
|||||||.|||||| ||| |
ACTTCGATGGTCGTCCTACAA

EST sequence (targeted fragment marked with yellow):
AAATAATCTACTTTTTATTCAACAATTCTCGCCAACATCCTGCTGGTAGCTTCAAGTAATTTCTCTCTTGATGACTTCAAACTTGGGTGTAATCAATGTTAATTTTTCCAGCGTTAAGGTCAGCAATCTGAGAGAAGGCTTCTTGAGAGAGATCAATGGTTGCTTGACATCCGGGGGATGGGCATCGATCAACAATCTTGACAGTGACGCTGCCACCTTTGCAGGGCTGAGGAACACCGGCGTTTGTAGGACCTTGCAGGTGACCTTGTACATTCTCCCGCATGCAGCACCGTTGGCCAAATTCCATCGCTTGCAGCCGCTATCATCACACCCTGATCTTGGATCATAGCATGCAGACGGAACATAAACGTTGTAGTAAGTGGAGTCCGGAAATGGCAGAACTCTGAGACAGCTGCGC


gi|38587697|gb|CK103372.1|CK103372 G114P38.5pR Populus tension wood cDNA library Populus tremula x Populus tremuloides cDNA clon 

TGAAGCTGCCAGCATGATCTG
||| ||||||| ||.| ||||
ACTACGACGGTAGTGCAAGAC

EST sequence (targeted fragment marked with yellow):
CATGGCATCCTTGGATGACTCAGTATGGGTCCCGTTCACATCCAAAAAGATGTTTGATTGTTTGTATTATAGAAGGTCAAAACGAGGTAGAGGTAAAATTAACTCAGAACGTGATGGCAGCATCAGAGAAGAAACTTCAATTGCTTGTAACCCTCATCTTGACTCCAGAGGGGATTCCTATGAAGGCACAATTGGTGGATCTCATAGAATACATTCCATGGGCTCAAAGGGAACACATGATGCAAATATTTGCAATCAATGATCTCCATTTGGGGCAGGGGACATGAATCAGCATGATACTTGTAGAGATACCCACAAATGGTTCTGTAAAGTAGATTTCAAGCTTCCATGCGAAGAATGGGGATCAAGTTCAAGGATGACAGTTCGATCGAGATCTACTAGAAACAGGAAAGCCAGTTGTCACTTTCATGACACAAGTCCAGTAGGATGGAAGGAGGAAGTTTGCATCAGTCA