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mature miRNA: AGAATCTTGATGATGCTGCAT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73889686|gb|DT492424.1|DT492424 WS02550.C21_L02 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02550_L02 3', mRNA sequence

AGAATCTTGATGATGCTGCAT
|||||.|||||||||||||
CCTTAGGACTACTACGACGTC

EST sequence (targeted fragment marked with yellow):
GTTTCAACAGCTCTCGTGTAGTGACATAGAAGAAAGAGAAAGGAAAAGAAAACGAAAGAGGAAGGAAATTTCTCTCCGTGTGATTTGTACAGAAACGGCAATGAACATAACTCACCAAATTATCAAACTCAGCATCCAAGCAGCAGCAATGGAGGCCGAATTGGAAGTGATCTTTCTCTCTCTCAATATGAGCTTCATTATAACCATCATTATCAGCAATGGCAAGCAGGTCCTCCCCGGTTTGCAAATGCTGCAGCATCATCAGGATTCCAACAGCAGATTAGGACTCCACAAAACTGGCTGCAGAAAAATGGGTTCAACTCTCTCATGAGACCTTCTTAACCCATACATAAAGTAGCCGTTCCATCTTCCTTGCTATGCAATCTCAATCCCCACCTTGTTTGCTAGCTGGGTAAAACATGTAAAGAGCAAGGCTTTTAACTACGAGGCAGGTTGTTTTTCAATCCACTGTCCTCCCAAGCCCAGAGAGAACCACCACCAAGAAAATCACGATATAGGGGCCAAAAAAAAAAAAAAAAAA



gi|73888295|gb|DT491033.1|DT491033 WS02546.C21.1_J24 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02546_J24 3', mRNA sequence

AGAATCTTGATGATGCTGCAT
||||||.|||||||||||||
TCTTAGGACTACTACGACGTT

EST sequence (targeted fragment marked with yellow):
GGACAATTTGGGAGATGGCCATTGTAGTTTTGGTGGTTGTGAAATACCCATCAAAGAGAGACAAGCGAAGGCTGAGGGCACTGCTGCTGCACGTATGGGTTTAGAGACGCTTCATGGTCTACCAATAGCATCGAGGAACCTTCCAACATGGTCTGGCATGTTCCCTGGTTTAGCATCAAGCTATGAGGAAAGGACTCCGGAGAAGAGGTTTGAAGCAGTTTCTCCACCGAGATTCTCAAGCTGGCCATGGCAGATAAATGGCAGCAACAGTGTTGCTGCTACAATGCCACAGCTCTGTGTTGCAGCATCATCAGGATTCTCTTCTTCGACCAAAACTGTTCCTTCAGCTACTCTTCCATTCAACCAACAGAACCATTTCGCCAACAACAACCCGCGACTTGCAGCCTTCACCACATCTGCCACCAACCCCTCGTTTTACTACTCTTATACGAGCTAATTAACGTTAACACCTTGAGTGCATTTGCCATTGTGCTAAATTAAGCGAGCAAGGGAACTGAATCAATGGAGCCAAAAAA