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mature miRNA: AGTATAGAGCAAGCAAGGGG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52494987|gb|CV242012.1|CV242012 WS02513.B21_O04 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02513_O04 3', mRNA sequence 

AGTATAGAGCAAGCAAGGGG
|.||||||||||| |||.|.
TTATATCTCGTTC-TTCTCT

EST sequence (targeted fragment marked with yellow):
TATAAGTGGAACTTGCTATTATTGCTAAGAAAAAAAAATAGTACTTTGTCATCTTACCTTAATTATAAACCGAATCCTAATTAAGAACCACAAAGCGCTCACTTCTTCCTCTGTTTTATCTTCTTTTTTTTTTGGTAAATCAAAATCATAGATGTACTGATATTTTTTTCCCTTTCTCTCTTTCTTTTTCTGGGTTTTCAATTTGGACCTTGGTTTTGTTTTATTTTTCATTTTCCAACCTCTTCAATGTCTCTGCTTTCTGCTCTCTTCTTGCTCTATATTGGGGTTTTGACGGCATCAGCGTTGTCTGATTGTCCTAACGAATCAGCTTTGTCTCTTCACCATATTCAATCTCAATGTCCTGTTTCGATCCCTCCAAATCCTCCTCTTCAGGTGGATGGGAAATTCCTGGACAGAGCCTTGACTTCAAAACAGAGGAATGCTTATACTTCTGTTTCTTTGGGTGAATCTTCTGCATCAGCTAGGTCACAATCCTGAGGCATATCCTGGTTTCCCTTGGGACATAAATCAAGGTAGGAAAGGCTTACTGTTAACTAGTGTATTACAAATAGTTTTAATGTTCTGGGATCATGTGTTCTCTCGCTCACTTGTGTTAAAATTTTACTACTATTAGCGTTGTTGATGATGATAATGATAGCTGAAACCATCGATGCTTGTTCTAGTTTTGGGAGGAAGCGATCAGGCATGTAAATTTGAGAACAGCTTTCCTTTTGTAATTTTTTTCCTTTGTATATTATTGTCATTTGGACGAAAAAAAAAAAAAAAAAA


gi|73886875|gb|DT489613.1|DT489613 WS02542.B21_L09 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02542_L09 3', mRNA sequence 

AGTATAGAGCAAGCAAGGGG
||.|||.||||  |||.|||
TCGTATTTCGTGAGTTTCCC

EST sequence (targeted fragment marked with yellow):
GCATTTTTTTTGGCGTTTTAACAGTGAGGAATTTGTTGAAATCGTGATTATGAAGCACGTTTTTTAATCCATTACCCTTCACCCTGAACTCTCAAAATATTTTCATTATATGCTGTAATTTTTCCTTTTCCATGTTTTTTTCCCCTGCTCCATTAACATATAAATTTCCTGAGCCATCTGCTGAAAACAAAAAAGACATGGTTCTCAAGCCTCTGGTTCCATGTCGATTGGCCAAGGAAAACAAAGAAGACACGGTTCCACTTATTTTACATACGATTCTGAAAGAAAACTAACTTAAGATCAAAGGAAAGAAATGGCGAATCTTGCCTTCTGCTCTAATCTTCTAGTACCCGTTTTCAAATCATCTGCCTTGATTCCCATATTCCCAGGCATCCCTTTGAGTGCTTTATGCTCCTTCTCAGGCTACTGCATTTCTTATTTTCTCATAAAATCCTTTGCATTATCTTTCTGTTCAGCGTTGCCCATTGTCTAAGCCCTCAAAACCTCGTAACAAGGCCGGTGAGTGCACATCTTGGATAGATCAGCACATCAAATTGCCTCGCAAATTACATGAATATTCTGTCTGAAATGCCTAGAATTAATGACACGGTCGTGCATGTACCATAACATTACATTTCAATGAGGTGGCACAGATCAATCACGAGCTTGAAGAAATTGGAGTGTTTTTATAGTGGAGGAAATACTATTGTCCAGCATATCTAAAAATTACAGAATACCCCTCTACAAAAATACATAATATTTATTTATTTGTTGTTTGCATAGTTAACCACAAAATTATTTCCATGAAATTATGTCAGTGATTGAGGAATAAGAAGGAAAAAATTAAAAAACTTGATTTAAAAAAAAAAAAAAAAAA


gi|52384144|gb|CV230539.1|CV230539 WS01916.B21_N22 PT-DX-N-A-10 Populus trichocarpa cDNA clone WS01916_N22 3', mRNA sequence 

AGTATAGAGCAAGCAAGGGG
|||| |||||||| ||| |.
TCATCTCTCGTTCTTTCACT

EST sequence (targeted fragment marked with yellow):
TGCCTTCACTGGTCCTAGTGCAGATGGAACTCTTTTATATCATATTAGAGATGGATTGAGTTCAGAAATGGTCAATATTTTTTATATGTGCTTCATTAAGTGGCATAGCAAAGCATAGTATTCTTCATATTAAGGATACTTTTTCTTACTTTGGCAATTACCGAACTCTGTTTTTAATCATTTTGCTTTAGGTTTTCCACTGTTTTGGCCAGTATTTCTGCCTTCATTTTGAAGCTTTCCAGCTTGGGATGGCTCCTGTTTATATGGCATTCCTCAGATTCATGGGTGATGAGACAGAAGCTCGGAATTACAGCTACAGCCTGGAGGTTGGGGGAAATGGTCGGAAACTTATTTGGGAAGGCATGCCACGGAGTATAAGAGATAGCCACAGGAAGGTTAGGGACAGCCATGATGGACTTGTTATACAACGTAACATGGCACTTTTCTTCTCTGGAGGAGATAGGAAGGAGTTAAAGCTGCGAGTTACAGGACGGATATGGAAAGAACAGCAGAACCCAGAAGGTGGGGCATGCATTCCCAACCTTTGTAGTTAGGCTAATTATCACTTTCTTGCTCTCTACTGTGCGATCTATTTCTTGTAATACTGTATTACTGTTACTAAAATGCTGATCATTTTAAGAATCCTCATGTACAATACTGGAGGCCCAAGCTTTATAAATTGTTAATAAACTTACTGATAAAACTGTCCAAAAAAAAAAAAAAAAAA


gi|73875257|gb|DT477995.1|DT477995 WS02521.BR_I23 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02521_I23 5', mRNA sequence 

AGTATAGAGCAAGCAAGGGG
||| ||||||||| | |.||
TCAAATCTCGTTCCTCCTCC

EST sequence (targeted fragment marked with yellow):
ATTTCTCTTGGAGGGTTATTGTTAGTTTCAAACATTCCCCTTTCTTGGCCATGGATTTTTGCCGGAATGCGACAGTTTCCACTGAGTATCACCAGCAAGAGAAGGTCCTTGCTTCCCCTCCTCCTTGCTCTAAACTTGGTGCTGCTGCTGCTGCTTTGACAGCTACTACTAGTCCTCTTGATGACCCTTTCTCTGCTCAAAACACGGAAGTTGATTTTAGCTCGGAATGGCTGTCAGTATTTGTAGAGGACTGCTTGTCCAGCACAGGAAACTACCTCCCAGCACCCACAGTTGAAGCTCAAAAGCCAAATACAGAAGAAAACCCCCCAAAAAACTGGCAACGGAAACCCCAAGACCAAGAAGACCCATCTTCCTTGAAGAAACTTGTAATCCCAGGAAAGTCAAGAAGCAAGAGGAGAAGGCTTCCTGGTGACAAGACCAGAAACCCATTAACTAGCTGGTGTTATACCAACCAAGCCTTTAATAATTTGACCAGCTCAGACCCTCCTTTGCTTCAGCAAACATATTGGCTAGCTGACAGTGAACTCATTATGCCCATAAAAGAGGACAGCAACAACACAGACATGGATAATGAAGTGCAAGAAGAATCAGGAGTGGGAGTGCACGACGAGGACATAGGAAAGGTAGTAGCTGTAGTAGGGAGTAATGGTAGCAAGGACAGTTTAGGCGTTTTGGAAAGCAACAATGGACAGCAGCAACCAAGGAGGTGCACCCATTGCCTAGCACAAAGGACCCCACAATGGAGGGCAGGACCACTGGGTCCAAAAACACTTTGTAATGCATGTGGAGTGAGATACAAGTC


gi|60701250|gb|DN491130.1|DN491130 UM63TD09.3pR Populus female catkins cDNA library Populus trichocarpa cDNA clone UM63TD09 3', 

AGTATAGAGCAAGCAAGGGG
|.|||||||||| ||| ||.
TTATATCTCGTTAGTTACCT

EST sequence (targeted fragment marked with yellow):
GGGCAGTAAAATGAAAGTTGAACAACTAATTAATCAGTCTCTGCCCTAATTTCTTGAGAAGAGCAGCCCGCCCCTAATTTCCTTCTCTGTTATCTTTTGCAAAACAAATAATTGGTTGAGATTTCATCAGAGAAATTGGAGTTTATTTCTACAGAAGGCTGAAAGCTGGGACCTGTTGATACGCATATACACCTTCCATTGATTGCTCTATATTGGACTGCCCATGCTGATGACAGTTTATGAGAAAGGGGTATCAGTCCTGATGCCCGGTGATGATGTTCCTACCTTCCTTGCACACCCAGCTCCCGCACCCTGCCGTCCCGAGCGCATGATCATACAACCACTCCAACAACATAGCCTATGCCACAGCTTCACCTCAACCTCTACTTCAAGCTAAAAACCTAACATGAATCAAAATAACTAACTAGAACAAGGGGTTTTTCTCTTCCAATCATTGCCATCAACCAGATTTGTTTTGAGCTGTGAGCATTACTTATCATGAATTTGTGTACATTTGAATTCAATGATTACGTATCCATTTGGAG