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mature miRNA: CCCAGAGCCAAGTGGCGCTG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52388562|gb|CV232727.1|CV232727 WS0198.B21_H20 PT-DX-N-A-10 Populus trichocarpa cDNA clone WS0198_H20 3', mRNA sequence 

CCCAGAGCCAAGTGGCGCTG
 |||||||||||||.|  ||
TGGTCTCGGTTCACTGAAAC

EST sequence (targeted fragment marked with yellow):
GACCATCAAAGTCACTTGGCTCTGGTTCACCTCCTGCAATCACTGCATCATTATCTAATGCATCCATGTTATTTGCATTTTCTAGAGGAGGATTAAGGGTTTCATAGCATATGACGCCCTCAAAACAATCTTCCAATCCCATTCTTTTGAGAACTTCAGCTGCATGCGCCTTATCAGCGTTTGTGAAGATTATTTTACGCTGTGGCAAGGAAAGTAGAAGGTTCCTTAGCACCGGATCAGGCTTTAGTGTTTCGTAAGGCAATCTTCCATGAACGAAGGCATGAAACTCATCGTCATCAAACTCATAGCCAAGATCCTTTAGACCGGCCATTGTTGTTCCATGTTCCCTGTATAATTCCAAGCACATCCTCGGAACTTCACTTTCTTCAATATGTAGCTTATGCAACATGAACTCTTCAATGTTTTTGCGGCAAGCCAAGTTGAGACCCAAGCTCATGGGGTACAGAGTATCATCCATGTCAAAGAGCAGGCACTCATATTTTGGTCCATTGGCCCTCTCAGCAGTGTCCATCTTAAAAAGTTTCCCTTCAAAG