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mature miRNA: CCCGCCTTGCATCAACTGAAT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|18017389|gb|BI136461.1|BI136461 F068P66Y Populus flower cDNA library Populus trichocarpa cDNA, mRNA sequence

CCCGCCTTGCATCAACTGAAT
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GGGCGGAGCGTAGTTGATCGA

EST sequence (targeted fragment marked with yellow):
AAGCAGCTAGTTGATGCGAGGCGGGGAGAAGAAGCTACTACCAATCGGAAAGCGATTCATTCTCTCTGATCTTAATATTTCTCAAGACTTGCTTTTCTTACTGTTGTGGAGAGCTAATAGCTCTGGGTTTGAAGGAGGCATTCCTTTTCTGCGCATAAGATGTACTTCTGGTCATCATCGTACCGGGATGCAGATCTTGAATTGGTTTTTATATATCAATGGACTGCAAGAAGTTCATGC



gi|73870020|gb|DT472758.1|DT472758 WS01227.BR.1_N09 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS01227_N09 5', mRNA sequence

CCCGCCTTGCATCAACTGAAT
|||||||.|||.||| |. ||
GGGCGGAGCGTGGTTAATGTA

EST sequence (targeted fragment marked with yellow):
GATAAAAAAGATTTCTCTTTTTAAGAGTTCATACCTTCTTGTATTGATTTGTCTGTGAATTCATTGTGATCTCTCAGCAAATAAAACAACAATGTCAACCGAGAAGGAGAGGGAGACTCATGTTTACATGGCCAAGCTCGCTGAACAGGCCGAGCGCTATGACGAGATGGTTGAGAGCATGAAAAATGTTGCCAAACTTAATTGTGACCTGACTGTGGAGGAGAGGAACCTCCTCTCGGTGGGATACAAAAATGTAATTGGTGCGAGGCGGGCTTCTTGGCGGATCATGTCTTCAATTGAGCAGAAGGAAGAATCTAAAGGAAACGACAGCAATGTTAAACTGATTAAGGGATACCGCCAGAAGGTGGAGGAGGAACTCTCCAAGATTTGCAATGACATCTTGTCCATCATCGATGACCATCTGATCCCCTCTTCCGCCTCAGGAGAAGCAACCGTGTTCTACTACAAGATGAAAGGTGACTACTACCGCTATCTAGCTGAGTTCAAGGCTGATCAGGAAAGGAAGGACGCAGCCGATCAGTCATTAAAGGGTTACGAGGCTGCTTCTGCCACTGCAAGCACAGATCTGCCTTCAACCCACCCAATTCGTCTTGGCCTTGCCCTTAACTTCTCTGTTTTCTACTATGAGATCATGAACTCTCCTGAGAGGGCCTGCCATCTGGCCAAACAAGCTTTCGACGAGGCAATTGCAGAGTTGGACACTTTAAGTGAGGAATCATACAAGGACAGCACCTTAATTATGCAGTTGTTAAGAGATAACCTCACTCTCTGGACTTCTGATTTGCCTGAAGATGGAGGTGATGATGACTTCAAAGGTGAAGAATCAAAGGAAAAA