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mature miRNA: CCGATTCTGGACTGGGCAGC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|60704995|gb|DN494805.1|DN494805 M127F05.5pR Populus female catkins cDNA library Populus trichocarpa cDNA clone M127F05 5', mR

CCGATTCTGGACTGGGCAGC
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GGTTAAGACCTTACCTGTAG

EST sequence (targeted fragment marked with yellow):
TCGGGGGCACCAACGAGGCCATGCTTGTTATTGAAGCATACCGGACCCTCCGTGACCGTGGCCCATATCCAGCTCACAAGGTTCTTCAGGATATGGATGGCAGGTTTGGATTTGTGGTCTATGATAGCAAGGCTGGACAGGTCTTTGCTGCACTGGGTGCAAATGAAGGTGCTGAGCTATTCTGGGGGATTGCAGCTGATGGGTCACGTTGTAATTTCGGACAACTTGGAGGTGATAAAGGGAAGTTGTGCTAAATCATTTGCTCCATTTCCATCGGCGTGCATGTTCCACAGTGAACAATGCTCGATCAGCTTTGAGCATCCAAGTAACAAAATGAGGGCATTGCCTACGATTGACAGTGAGGGAACAATGTGTGGGGCTAACTTCAAGGTTGATGTCCATTCCAGAATTGGCACTATGCCTCGTGTTGGTAGTGAAGCCAACTGGGCTTTGGGAGGTTCACGAGCCTAGCTAATCAACTCTCCAAGGGATATTGTGATGACTGATGATTCCTTTTTTACTCT