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mature miRNA: CTTGGGGTGAAGGGAGCTCCT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73877475|gb|DT480213.1|DT480213 WS02528.BR_D24 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02528_D24 5', mRNA sequence 

CTTGGGGTGAAGGGAGCTCCT
|||| |||||| .||||| ||
GAACACCACTTATCTCGAAGA

EST sequence (targeted fragment marked with yellow):
GCAACCATCACCTAACGGTGATGAAAGCTCCAAAGAACTGGTTAACAATGATGACGAGCCAATGGAGTCTGAAGATGACAATGCTAACAAGACTAACGCAAAAACTGAAATTACCAGGCAAAATGAGAAGCTCTATTCACCACAAGGTATACTGAATACAAAGATGAAAAGAGCACAGAACAAAAGGAGGAAAAAAGCGGCTAAAGTGGATACAATGGATGGTGATTATGACTTTAAAGTGGATTATAACAAAGGTTCGGCCATGAATGTTGAAGACAGGAGTGGTAAGATGGATGATGATAGTCCAATTCCTGCTGAGGTGCCTATGTCTGGTATTCAATGTGATGATGAGTAATGATTTACCCTTTTTGGGGGGGGACCGTGGAAATACAAACTCTTTCTGATTCCAGCAAATTGGAAATTTTGTTAAACAACAGAGCGAGATTAATTTATTTCATATGTGCTCGTACCATGCCTCGATATCATGAATTCCTTTTTGGTGGGTGGCGGCGGGTAAATTAGAAGAAAACACAAAATTCATTCTCCACAGATAAATTCCATGCTTTTTACCTCTACTACATCTTGATGTAGCATCGAAATTATCCAAAAAAAAAAAAAAAAAA


gi|24063505|gb|BU871981.1|BU871981 Q037E05 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence 

CTTGGGGTGAAGGGAGCTCCT
|||||||||||.||||| ||.
GAACCCCACTTTCCTCGTGGG

EST sequence (targeted fragment marked with yellow):
GTGACAAGCGTAGAACTTAAAAGTTGTTCCCGAAGAAGAAGATCGGCAAAGCTGCGGAAATGAGCTTATCTGCCAATCAAGAAAGGGCCGAAATGGAGAAAAAGAGGCTCGTGTGGAAAGCAGAAAGATCATCAAGAAAACAAGCAGCCCTGAGGGGTGGACCTGTTGATCCTGCAAAACTGGTGGTGGAACTTGCTCCGATGGAAATCCGCACATTTGTTATTGACTTCGATCACCAGTTCCGCCGTGTTTTTGCTTCATAGATGCTCCACACATAATAAATCCTCCTTTGCTTATTGGAGTCGATTTTCGAGGCCTGAGATGGGTGCTCCTTTCACCCCAAGCGTTTCGCTCTCAAGAATTGCTCACTTGTATGAGCTATCCATGGTAGTTTAGGTCACTCTCTGCTTCTTAACATGAAATCATGAAATCATGTAGTCCAGGAAACAGAGTTGTCAGAAATAATGTTGTTTCTTCCCTGTTCACTTCCAACCTCCAAA


gi|60701063|gb|DN490963.1|DN490963 UM42TG12.3pR Populus female catkins cDNA library Populus trichocarpa cDNA clone UM42TG12 3', 

CTTGGGGTGAAGGGAGCTCCT
|.||||.||||.||||| ||
GGACCCTACTTTCCTCGTGGT

EST sequence (targeted fragment marked with yellow):
GAGGTTGCTAACCTCATGAAGAAAAATGGTATTGATGTCTGTTGTTCTTTAGAAACTAAGTTTGCCTTATACAAGCTTCAATTTATGCACAGGTTTCGGTTGAAAAATTGGAAGTTCCTCTCCAATTTGGATGTTGCACGCTATGCAAGAATTGTTGTGTTTTGGAATCCTGCCACTGTTGATATTGAGCTTCTTGCTGTCTCAGAACAAGGACTCCATGTTTCTATCAGGTGTTTAGTCAGCCACCTTGAGTTTGTCGCCACCTTTGTTTATGGTCTTAACACCATTACAGCTAGACGAGGTCTATGGAATGATTTGCGGCAATGGCTCCTGCAATGCCTTGGATTATCATGGGTGACTTCAACTCCATTCTCTCTCAGCAGGACAAGTATAATGGTGAACCTAAGCTGCTGCCTGATATGGTCTTATTATGGTCTCTTTTGCTTTGAACATGGTACTTGCTGCTGGGTTCAACGGTTGATGATTAGATGAATATGAGCTGTTATGGTGCTCCTTTCATCCCAGGTTTCTGTCTGTTGGTTTTCATCTAGTCTGCTGTGTACTGCTTGCTTGCTGTTGCAGCCCGATTGTTATCTGCTGTACTTTTGGGAAGTATATGACTCACTTCCTTCTTTTGTTGGTTAACTTCAGCACCTATTACACTTATAGGTTTCTTAGTTAACCAATTTCATCTGTGTTCCGTACAAT