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mature miRNA: TAGAATTGCAGTGCCCTGATT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52493625|gb|CV240650.1|CV240650 WS0251.B21_P12 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS0251_P12 3', mRNA sequence 

TAGAATTGCAGTGCCCTGATT
||.|||||||| |||||||||
ATTTTAACGTC-CGGGACTAA

EST sequence (targeted fragment marked with yellow):
GATCCAAGTTTAACAAGTTTCATTGACACGTCATACTACAACAACATCTCTTTATTAATCTTTAAGCAGCATCCAATAGCTCAGCCAACCAACTATAAATGGATGGCAAAAGAGCTCTTGAGCCTCAGTATACCTTCAAACCTTGATCCCATCCAATCAGGGCCTGCAATTTTATGAAGCTAGATAAAATGAAAAATGTGAAACAATTAGTATAGCATATAGTGCATGTACCTGCTGTGTTCATTCACTACAGGCAAGGGTATCGATCCTTGAAAGAAGAGGCAGCTCGATCAAATAAGAGACATTTCAAGAAAACCTTGACACAGAATCTTCATCCATTGTCCAAACAAATCCAGGCATGTCAAACCTAGGCCCCGCAATGGTTCTGTATATATAAAGCTTCTCAACAGGGCAGCTTTCCGGCCTCAAATCTGGAGGTCCCCTCTCATCAATAACTTCAACATTGAGTCTAGGCATCTTCTGACCCAGCAGCTTACATGCTCTGAAACTCACAGAGCAAGAAGACATCCAAAGGGATCGCATTGTCTCCAGCTTTGCAGCATTGGCCAAAAGAGCTTTGTCACCAAAGGGACAATCCCTTATCTCTAGTTTGCAAAGGTTTTCGCACCCAGACAACACATGGTGGAGTCCCAGATCACTATCCCCAGCAAATGCCACAGATAGCATCTCTAACTTCTTGGCGTAAGTTCCAATGTACTCAAACACGCGATCAGTCAATAGACCTGAGAGGGAAAGACGCCGGAGATCCTTGTAGTTTTCAACAATGGCTCCAAAGCCCAAATCCAGAGGCTGGAGTGTTTG


gi|52401642|gb|CV239205.1|CV239205 WS0231.B21_K14 PT-MB-A-13 Populus trichocarpa cDNA clone WS0231_K14 3', mRNA sequence 

TAGAATTGCAGTGCCCTGATT
|||||||||||||||||||||
ATCTTAACGTCACGGGACTAA

EST sequence (targeted fragment marked with yellow):
GAAAAATTAATTTCTGTTCACTGACCATTTAATGTCCTAGTTTTGAATCTGCTATTCAAATTGAGTTTTAACTATACTCTTGTGCCCTCTGTAAATGGTTTTAGAGTCTGAAAAACTCAGACAGTGTGTGTTGTCATCAGCTTCTTCTCCCGCATAACCCACGAGATTGGAGTTAAATAACAGCACTACATCCGTTTCCTTTGTTTTTCTTAAAGGATATTGCTCTGCATTTATTGGTACAAAGAACATTTTGTTCAAAATGATTATTAATGGATTTATTATAAATTTTTCCAGTCAGTCTAATGTTTGGAATAATATAAAAACGAGCCTTATTACAGATTGTAGACTTGATAACCAGGATTGCAAATTCAAAATTTCTTAGTGTGAATGTAAGATGATGGCAAGCTGGGAGTCTCTCGTGAACGAAGGAGGCCCTAATCAGGGCACTGCAATTCTAAATGTCTGTTGGCAAGTGGCGATGCCAGACGTTTGAAATTTACTTATCATTTGTAGACTTGATTAGCCCCTAACAGTCAGAATTGCAGTGCCTTGATTTGGGCTTTGTCTGTTCTTGTAACCGCCATGCTAGGGGAGGGAAATGCTGCTGCAAATGTGAGCGCTGCTACTGTTAGGAATGAAAGAGATGCTGCAATCTTCGTGAGATATCATCAAATATGCTATTTTGTTGGGGAATCATTCAGCAGATACTAGGAAGTTGCTTATGTTGTTAGCTTTTCCTTGTAGTGGTATTTATACTGCCTATTTAAGAGAACTTATTTTGAATAAGATGGCAAGTTTCAGATTTGTGCTAAAAAAAAAAAAAAAAAA


gi|38575574|gb|CK091249.1|CK091249 F066P02.3pR Populus flower cDNA library Populus trichocarpa cDNA clone F066P02 3', mRNA seque 

TAGAATTGCAGTGCCCTGATT
||||||||||||||||||| |
ATCTTAACGTCACGGGACTTA

EST sequence (targeted fragment marked with yellow):
AGTGTTTCTGTTCATTGACCATTAATTCCTAGTTTGATCTGCTATCAAATGAGTTTAACTATACTCTGGGCCCTCTGAAATGGGTTAGAGTCTAAAAACTCAGACAGTGTGTGTGTCATCAGCTTCTTCTCCCGCATAACCCACGAGATTGGAGTTAAATAACAGCACTACATCCGTTTCCTTTGTTTTCTTAAAGGATATTGCTCTGCATTTATTGGTACAAAGAACATTTTGTTCTAAATGATTATTAATGGATTTATTATAAATTTTTCCAGTCAGTCTAATGTTTGGAATAATATAAAAACGAGCCTTATTACAGATTGTAGACTTGATAACCAGGATTGCAAATTCAAAATTTCTTAGTATGAATGTAAGATGATGGCAAGCTGGGAGTCTCTCGTGAACGAAGGAGGCCCTATTCAGGGCACTGCAATTCTAAATGTCTGTTGGCAAGTGGCGATGCCAGACGTTTGAAATTTACTTATCATTTATTAGTCAGAATTGCAGTGCCTTGATTTGGGCTTTGTCTGTTCCTGTAACCGCCATGCTAGGGGAGGGAAATGCTGCTGCAAATGTGAGCGCTGCTACTGTTAGGAATGAAAGAGATGCTGCAATCTTCGTGAGAGATCATCAAATATGCTATTTTGTTGGGGAATCATTCAGCAGATACTAGGAAGTTGCTTATGTTAGCTTTTTCTTCAGGGAGAATCTTATCATCTGATTGTGATACCTATTTCCTTGTAGTGGTATATATACTGCCTATTTAAGAGACTTATTTTGAATAAGATGGCAAGTTTCAGATTTGTGTTATTGCCATTTTCAAAAAAAAAAAAAAAAAAAAAAAA