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mature miRNA: TGAAGCTGCCAACATGATCTG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|24068916|gb|BU877392.1|BU877392 V033E03 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence

TGAAGCTGCCAACATGATCTG
||||| ||||| || ||.|||
ACTTCAACGGTGGTCCTGGAC

EST sequence (targeted fragment marked with yellow):
CGGCCGGGGGAAAGAGAGGACCGAAAGATTAGAATTCGAGATCCGAGAAAAATAAAAGCAGAGTGAGTGAGTGTGTGGCTGTGCCAGTCCCTCTGTTCAGAAAAAAGCTCCTCCGCCTCAGGTCCTGGTGGCAACTTCACAACTGCCGTCGTGTCACTCCTCCACGTATACAAAAGAAAATAAAGACAGCTTTTGCTCTATCCAAGACATTGGCAGACTTCCCTTAGATTCCTCGTCCCGATCTCCAACTCCTCAGAATTTCGTCATTTCCTATGACTTTGTCTAACTGTCTGTAATCACATGCATGAATTTGCCTGTCTCACTTTCTTTTAATTCCAAACCGAAATCGCTAATCAGATCTCGGGTGTGATCAAGATGTCATTTAAGGGAAAGTTAAAGGAGATTAGAGATGACATTTCTACCACATTTTCGATGGGAAAGTTACAGAGGAACAGAACAAATCGAAGAGGGAGAGCTTATATTGCGCCTGAATTAGGAGGTGGGTCTGAGTTGTTTAGGGAGTTGATTGAAGAACAGAGTCCGTGGGCTAATTTGCCGCCTGAATTGCTACATGATGTGA