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mature miRNA: TTATTAAACCCGGACCGGTCCAGC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73887099|gb|DT489837.1|DT489837 WS02543.B21_F04 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02543_F04 3', mRNA sequence

TTATTAAACCCGGACCGGTCCAGC
|||||||| |||| ||.|||||.|
AATAATTTAGGCCAGGTCAGGTTG

EST sequence (targeted fragment marked with yellow):
CAGAACCTGATTACACAAGTCAGAGTCGACGACCGGCTGCATATCAGCGGAGGATCAAGGAAAATATAGGTAGAGCTGATGGATGAGGTCCAACCCTTCTGTTGCCTGAATTGTACCTTTCAGATTTGGACATCCGAGATTTAATTGCTGGCATGTTTGAGTATTCTGTGGAAAGCGGATATGGAGGTTGAGGTTTATTTCAGTGTCAGAGAGTTCTGCTGAATATATTAATGTATACGTAGCAATACCTAATGCATTCTTCATTCGATTCTGATCAAAGCAAATATTTACATGGGTGTGTGGAATAGAGAGCAATATTGAGTCTGTTGTCTATTAGTGAATCCAAAGGTTTTAGACATTACTATAGCAACTGTGTAGCTGGAATCCTTTGAAGATCTGGTTTTGGTTAAATCCCTGATCGGATTATCCGTATCTCGAAGCATGGCCCGGTATTTGATTAATTGGATCAACTAGCTTGATGCTGCTTGTTTGTGAATTGTGACGGATTGCTGCTTTGTTGAGGTGCCGTTAAGCTGATTGGTGACAGAAGTACTCCACCACATCATAGCGCGAGATGGCATTATATTGTTTTCATATTCTACACTGGAAGTCTTGAGAATTGTATTATAATTGTTTTTCCTTGACCTGATTGCTGTCAACAAATTATTGATTTGCGAGTTTATTAATATTAGCTTTGGTGAATTCTGAGTTGAATGTTTTTTATACCTGGTCCGGTTACATGCTAGGTTGGTTAACCTGCCGTGTTGGACTGGACCGGATTTAATAATAATTGCCGAAGACTAGCTATATTATTTTCTTTGGCAAAAGAATCATTCCAAAAAAAAAAAAAAAAAA