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mature miRNA: TTTCTGATGGTGCTCTTTCA
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73882344|gb|DT485082.1|DT485082 WS02527.B21_D20 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02527_D20 3', mRNA sequence

TTTCTGATGGTGCTCTTTCA
||||||| |||.|| .|.||
AAAGACTCCCATGAAGAGGT

EST sequence (targeted fragment marked with yellow):
TTGCTAAAGCGAGTAAGGGCTGCTTGTCATTACCTGATATCGGTTCCTTCTACTCAAAGGTTCAGAAGCCATCACAGCAGCGCATTTATGGCCCAAAGACTGTGAAAATGATGTTGGGAAGAATGGAGAAGTACCCTCAGAAACCGTGGCCCAAGGACCAAATATGGTCATTTAAGAGTTCTCCAAAAGTTTTTGGCAGCCCAATGCTAGATGCTGTATTGAACAAGTCCCCACTAGACAGAGAAATGGTACATTGGTTGAAGCACAGACCTACAGTATATCAAGCGATGTGGGATCGATGAGGTCTTTCCCCAGATTGGGTAAAGCTGCAGGGGGGGTTATTAATTAGGCAACAACAGCTTGTAAGTGTTCTGGAATGATCTCTGCATTTTAGTTCACAGCTACCCCTTTTAGCATGGGAGTTGGGTCATCATTCCAAAACATCTTTCCCTCAGCTGCTTTCTCCTCATTCTTGTGTACAGTTTTTCCTTCTTTTCAATCTTTCCTCCTTGTGATTTTGTAATTCATTATTTGGTAGCAAAGCATTTATTTTTCCTTCTGCTTCAAGTAAAGAAGAAAAAAGAAGAGAAAGTTTACCATCAAAAAAAAAAAAAAAAAA



gi|24071246|gb|BU879722.1|BU879722 V063F02 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence

TTTCTGATGGTGCTCTTTCA
.||||||| ||.|||||.|
GAAGACTAGCATGAGAAGGC

EST sequence (targeted fragment marked with yellow):
TTCTGACGTTGATGCTGATGCTCTTGAGGAAAACAACAGCATACTCGGAAGAGTACGATCAGAAGTGCTATGATACATGTTTTACAAGCTGTGTTGATCAACGTAATATACCGCGGCAGCGTTCGTCTCACTGCATGGACGCGTGCAGCAACAACTAAGATGCAATTCCCTACTGCGATGTTGGTTGGGCGCTTCAAAATTGCAACAAAATCATGGACGATGAGGTGAAAAGGCATATTTGCTTGAATGAGAGCTCGAACACTGACTGCAACCCCAAACACTTGAAGAAGTCTCCGTAGCATCTGCTCTTTCATATTTGGAGCAATATATCTACAAGTAATGTCATGATAAATAATCACATGATTACGGGATGAACCATCCTTAATTATTGAGTAATTAAACCCA



gi|38586054|gb|CK101729.1|CK101729 F113P67.5pR Populus flower cDNA library Populus trichocarpa cDNA clone F113P67 5', mRNA seque

TTTCTGATGGTGCTCTTTCA
|||||| |||||||||||||
AAAGACAACCACGAGAAAGT

EST sequence (targeted fragment marked with yellow):
TCTCTCTTCAAGTCTTCCTCTCTCTCTCTCTCTTCAAGTCTTCCTTCAAGCAATCTATCAGAAAAACTTCCCATTTTGGTTTTCTGATATTTATTTACATCCTCAAATCCTTCTAGTTCATTATTCTGTTTCCTTTCTTGTGTTCTTTGCTTCTGTTAATTAAATTCTCCTGAACTTTATCTCATTTCTGTTCAACAATGGCTCAAATGAAAGATGCCCACATCGTAGAAATCCCAGCTAGATGAAAGAGCACCAACAGAAACCTGATATGTGCCATGAACACAAATAAACAGACAAATTCAAAACCACCCAT



gi|73882950|gb|DT485688.1|DT485688 WS02528.B21_O11 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02528_O11 3', mRNA sequence

TTTCTGATGGTGCTCTTTCA
.|||||||||| |.||.| |
GAAGACTACCAAGGGAGACT

EST sequence (targeted fragment marked with yellow):
CAAGCACCAGCAGCTTCATTCAGAGTTCCTACAGCCAATGCCTCACAAATCACCATGAATCCATCTCAAGATGGTTTAGATATTGGACTTAGGCATCTAGGATCAGTTCAACCAACTGGCCTTAGAATGTACAGGTCTCACCGTGAGGGAGTTGTACCTGAGACCACCCTAAGACATCGCAACCTCCCTCAGTTGAGAGTATTGCCAACAGATGGGGTAGCAATATTAGGGTTCCCTGACTATTATGAAGTAGAGAATCATGTTGACCACCACAGAGATATGCGCTTGGATATAGAGGACATGTCTTATGAGGAGCTTCTTGCACTAGGGGAGCGAATTGGCAATGTAAATACTGGATTGTCAGAGGGAACCATCAGAAGCCAATTAAAAACAAGGACTTATTTATCATCTCCTTCAATCAATTTGGAAGAGGCAGCTTGTATGGATCAAGAAGCTGATTCTTGTATTATTTGCCAGGATGATTATAAGAGCAAGGAGAAAATTGCAGCTCTTGATTGTGGACATGAGTATCATGCAGTTTGCTTGAAGAAGTGGCTACGCTTGAAGAACGTCTGCCCCATCTGCAAATCAGAAGCCTTAAACACGGAAAGAATGGATGTTTAAGGTGGCGATCTTGCTTTATATAAAATGAAAGGAAGAATAAAGTAGAGGAAAGGGGTGACAGTTTCTTTCTCATAATTTGTTTATTATAACGAAGTTGATATTTTGGCATATGTATTTTGTATATGCACTTGCAAATATACGACTAACCATCACACTAAGTCATTTATTTGTTGGCTCTGTATGTATTAAACCTGTACATACATTCATTATGCATTTCGTGTGATGTATTATAAACTTTCAGTTAATGTGCCAAAAAAAAAAAAAAAAAA



gi|73887828|gb|DT490566.1|DT490566 WS02545.B21_F02 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02545_F02 3', mRNA sequence

TTTCTGATGGTGCTCTTTCA
|.|||||||||||. | .||
AGAGACTACCACGGTACGGT

EST sequence (targeted fragment marked with yellow):
ACGCTGCCAAAATTAACATAAACGACAGAGTTGGATTTCTGGGAATCCAGCCATTGGACACATTGAACTTCTTCTTTCCATAGATTACTTCCAATAGAATTCAGATCATCTTCTTGCATTTGATTGAGAAGCAATTGAAGTGGACCAATCGCATAGACACGAGGAAACATGGAGTAAAGGGCATTCAGAACCTCTTGCTCCAAAGCATCAAAAGTATGGAAAATAACGGCAGAACCTTCAGAAGCCCTCTCAGCACATTCCACGCCGAAGTTAAACATATAATCATTTGGATCTGTTGTTCGAACAAAACTGGGAAGGTCCCTTAACTTTATATCTCTCATTCCTGGGATCCAATCTACCACTTTGTCCAAGTAGCCATTTGTTAAAAAGCTCTCATCTTCTAGTGGTGTCAGACCTTTTTCTTTGAGTGCTCGAAATTGTTTAGTCCCCATGAAGCTGCAAGCAGAGATAGTATAGAACAATGCAATAGGAATTTCGTGCATCGCAGCAGCATCGATCGCGACTGGCATGGCACCATCAGAGACGATATAAGTCACTGGGGGGACATCAGAAGATGCTGTGTCATTCACCTTGTCAAGCAGGTCATTAAATGGACCCAATAAGTTCTTCCTGGAAGCCTCACAAATTGCATAGAAATCGTGGGCGGCATTCTCATCCGGAGCAGGGAAGCCATCTGGAATGGATTCAAACCGAAAATCGGGCAAACCAGTCAGGGAATCAGGACCTCTAGATCTAAGTAAACGTTTGTGGTTAAACTCTGTATTGACAAAGGTTATGTGAAAACCTTTGTGATGTAGTAGTTTCGCTAGTTTAAGCATTGAGTTTATGTGGCTCTGAGCTGGGTGGGGAATGCAAACTGCATGAGGCTTATCAACTAA



gi|18019641|gb|BI138693.1|BI138693 F113P67Y Populus flower cDNA library Populus trichocarpa cDNA, mRNA sequence

TTTCTGATGGTGCTCTTTCA
|||||| |||||||||| ||
AAAGACAACCACGAGAA-GT

EST sequence (targeted fragment marked with yellow):
AATCTATCAGAAAAACTTCCCATTTTGGTTTTCTGATATTTATTTACATCCTCAAATCCTTCTAGTTCATTATTCTGTTTCCTTTCTTGTGTTCTTTGCTTCTGTTAAGGAAATTCTCCTGAACTTTATCTCATTTCTGTTCAACAATGGCTCAAATGAAAGATGCCCACATCGTAGAAATCCCAGTAGATGAAGAGCACCAACAGAAACTGATATGTGCCATGAACACAATAAAAGCAATTCAAAACCACCCATTAGCAGAAATTTCTAATAGTCCGGGCCATCTTTTGCTTCTCAAGCTATGGCAAAGAGAAGAGGA



gi|38575512|gb|CK091187.1|CK091187 F057P19.3pR Populus flower cDNA library Populus trichocarpa cDNA clone F057P19 3', mRNA seque

TTTCTGATGGTGCTCTTTCA
||||| ||||| |||||.||
AAAGAGTACCA-GAGAAGGT

EST sequence (targeted fragment marked with yellow):
AGGAAAAAAGGAGAGACTGCATGAGCATGGCAGTAGATTGTGGTACAGATATTGGAAGCATCTCAGGGTCAAAATGATGCTGGTGTTTGTGAAGATGAGAAATTTGGGTTTGAACGTGGCCCACTGTTTACTTTGGATACATTTCAGAAATATGCTGATATTTCAAGGCCCAGTACTTCAGGAAGTATGAGAATACTATTAATAAGGGAGGCGATATGACAACGTTTCAGAAGACTTGTGAACCCACATTGGATAATATTGAGGGCGAATATTGGCGAATTGTAGAGAAAGCAACTGAAGAAATTGAGGTCCTTTATGGGGCTGATCTGGAAACTGGGGTTTTTGACAGTGGGTTTCCAAACACATCTAATGAAGTTAGTTCTGCAACAAATGATCGTTATACAAAATCAGGCTGGCACCTGAATAACTTTCCGAGGCTTCCTGGATCCATTCTTTCCTTTGAAAGTGGGGATATATCCGGTGTTTTGGTGCCATGGCTGTATATAGGGATGTGCTTCTCCTCCTTCTGTTGGCATGTTGAAGATCATCACCTATATTCACTGAATTACATGCATTGGGGCGCACAAAATATGTGGTATGGTGTGCCAGGGAAGGATGCCATTAAATTGGAAGAGACCATGAGAAAGCATTTACCTGATCTTTTTGAAGAACAACCCGACTTTCTTCATAAGCTGGTGCACCCAGCTCTTCCCCCAACATAATAAGATCTGAAGGAGTGCCTGTACTATGCGGTGTGTTCAGTAATTCCGAAGAGTTTGTGCCTAACCTTTCCCGCGGAG



gi|24067026|gb|BU875502.1|BU875502 V008A01 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence

TTTCTGATGGTGCTCTTTCA
||||||||||||| |||||
AAAGACTACCACGTGAAAGG

EST sequence (targeted fragment marked with yellow):
CGGCCGGGCTTTGTAGAAATTTTAGTTTGATGCAAAAGTATCCTGAACTACGGCATCTGGAATGCTTGCTCTTTGTCGATTTATGATTATTGATCCAGATTTTTGTGATGCAAATCTCCAGCTTCTCTTCACAGTTGTGGAAAGTGCACCATCAGAAACTGTTCGTTCAAACTGCACTATTGCTCTTGGAGATTTGGCAGTTCGGTTTCCTAATCTGTTAGAACCATGGACTGAAAACATGTATGCTCGCTTACGAGATCCTTTGGTTTCTGTTAGGAAAAATGCTGTGCTGGTGCTTTCACATCTCATATTAAATGATATGATGAAGGTTAAAGGTTATATAAATGAGATGGCTATACGATTAGAAGATGAACAGGAGAGGATTTCAAATCTCGCTAAACTTTTCTTTCATGAGCTGTCAAAGAAAGGAAGCAATCCTATATATAACTTACTTCCAGATATACTTGGCAAATTATCCAACCAGGAGCTGAAAAGGGAGACTTTCTGCAACATCATGCAGTTTTTAATTGGGTCCATTAAAAAGGACAAACAAATGGAGTCTCTTGTTGAAAAGCTTTGCAACAGGTTCAGTGGAGTGATAGATACTAGGCAGTGGGAATACATCTCGTATTGCCTCTCCCAGCTGGCATTTACTGAAAAGGGAATGAAAAAACTCATTGATTCA