miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: Browse




mature miRNA: CCTCCTCTTCTTCTTGCTGTT
species: Populus trichocarpa x Populus nigra
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52523120|gb|CV270145.1|CV270145 WS0151.B21_D11 PTxN-IB-A-6 Populus trichocarpa x Populus nigra cDNA clone WS0151_D11 3', mRNA 

CCTCCTCTTCTTCTTGCTGTT
||||||||.||| | ||||||
GGAGGAGAGGAA-ATCGACAA

EST sequence (targeted fragment marked with yellow):
TGCCCTTAATATTAAATCTGTGGGGTATGAGTTTCTTGAGGAAACACTGGGATCTAAAAGCCAGCTTCTTCTTGAATCAAACCCTGTCCATAAGAAAATCCCAGTTCTGATTCATGATGGCAAGCCCCTTTGTGAATCTCTTGTGATAGTGGAGTACATTGATGAGGTTTGGTCTTCTGGACCTACCATCCTCCCCTCTGATCCTTATGATCGTGCCCTTGCTCGGTTTTGGGCTGCCTATCTCGATGAGAAGTGGTTTCCTACGATGAGAAGCATTGCAACAGCTAAAGGAGAGGAGGCTCGAAAGGCGTTGATAGAGCAAGCAGGAGAAGGGGTGATGATGCTGGAGGATGTATTTAGCAGGTGTAGCAAAGGGAAGGGTTTCTTTGGAGGAGATCAGATCGGGTACCTTGACATTGCATTTGGTTCCTTTTTGGGGTGGTTGAGAACCACAGAGAAGATGAATGGAGTGAAGCTGATTGATGAAGCTAAGACTCCTAGCCTGTTGAAATGGGCAACTAGTTTTTCTTCACATCCTGCTGTGAAGGATGTTCTTCCAGAGACAGAGAAGCTTGTTGAGTTTGCCAAGGTTCTTGCCAAATTCAAAGCAGCCAGCTCGAACTCCTAAATAACCATTATTGCCTTGTGGGCAACTTGAAATACTAGAAATTAAGGTTCGTATGTAATTTGAAAGACTAGAAATTAAGCTTGTTGAGGAGAAATAAATTTAGATTACTTGCTGTGAACAATGATGGGGGCTCTAGTAATTTTGCTGCACCATTTTGCTTGAAATTAAGAAAACTTGTTGAAGTAACGGTACAAAATAATCAAACAAAAAAAAAAAAAAAAAA


gi|73943397|gb|DT526705.1|DT526705 WS02048.C21_L24 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02048_L24 3', 

CCTCCTCTTCTTCTTGCTGTT
|||| ||||||||||| ||||
GGAGCAGAAGAAGAAC-ACAA

EST sequence (targeted fragment marked with yellow):
GATTTTGTTTCTCTCACTCCTCCTCGTGCTCAGTTCCTATATTTTTGTTTCTTAGCTTGTTCATGTCGAAATGAGAGACAGAATGGAAAGGCTTGTTCTTCTTCCTTTCTCCATTGGTTGCGTCTCTGAGTCAAGTGTGGCCATAGGCGTGCACCAAACTAAAAGAGCAAAGACAGACACAAACTTATCTGCGTCAAGTCTCTCTCTCTCACACACACACTCACAAGATTGTTTTTACCTTCTTTTTTCTTTTCTTTTGTTCTTTCGAATCTCTGCAAACTTGATTATTTGATTTATGTTTCTTTGTCCTTGAGAAGGAACACAAGAAGAAGACGAGGAAAGCTCATCAAGCACAGAAAGTACGAAAAATTCGTTGAAGTTGCTTGCTCTTTCAAAGCCTAACGTATCCACTGGGTTTAATAAGCTAGTCAAGGGTTTGAAGACCTTTCCTCAGTTATTTGCGTACAAAGAAGAGATGGAAGAATTGGAAGTGGAAATGGAAATTGGGTTGCCAACAAATGTTAAGCACGTGACACACATAGGGTGGGATGACTCACCAAATACTAACCCCGTTCAGGGCTGGGACAGTCTCATTTCTACCGATTTACTTTCCCTTCAATCTGCAACTTCAAGGCAGTTCGAGCTTGCCATAGCAGCAGGACAGGCTAATTCACCCCTTGTTAGGGCTTCCTCTGCTTAATCCAAACATGACTATAGAGATGACAGTGGATATTGTTGACCAATTGTGATGTTGGTGGTGGATATATATATGGATATATATATGTATATGTAAAAAAAAAAAAAAAAAA


gi|52522451|gb|CV269476.1|CV269476 WS0208.B21_F19 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS0208_F19 3', m 

CCTCCTCTTCTTCTTGCTGTT
|||||||.||||||..| |||
GGAGGAGGAGAAGAGTGCCAA

EST sequence (targeted fragment marked with yellow):
AGCGGTCGCAGACCTCAATTCCACTACAAGCTCCAACCTGTCATGTGCCTTCTCTGATGATAGAATAAGCCCCCCTGCAGTGGCTGCTGTTAATCTTGTTGGGAAGAAACTACCTGAGGCAAAAGACCCATTTGTAGAATCTGAGATAATTGCAAGTGATGATCTTATGGAGCCAAGCTTTCACAAGTATGTAACCGTGAGAAGAGGAGGAGGTACATTGTGCGGGGAAGACATGGATGGTCAAGAATCCTCAGGAAGTAACAGCTTTGTTGGTGGCAGCCAACAGCATTTAGGATTGTCTACCTCTTCATGGGAACCCAATTCAGCTGATTCAACCGATCGCTGGTCTTCAAGATCCAACACGAGAGATGAGGATGACAAGAGTCCTCTGGGATATCAAAAGCATGGATTACCTGAAACAGGGCGTGACGTGGAACAGGCCAGGAGAGCATTCAGTGGACAAAGGACTGGAATTGGACGTGGGAGACATGGTACAAGTAAAAATGCTCACACAACTCCTATCCTTGCAACTGCTACTCAAACGTAATCCACTCAGTTGTGTTGTTTTCAAATTCCACGCAGTTGTGGCCCTTTTTAACTTTCAGTTTCAAAGGTTACTTCGTTGATGGTTGCAAACTTGCAACCTGTTTTCTTGTTAATTCAAATAGATTCATTCTTCCCTTGTTCAAAAAAAAAAAAAAAAAA


gi|73937866|gb|DT521176.1|DT521176 WS02033.B21_J13 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02033_J13 3', 

CCTCCTCTTCTTCTTGCTGTT
||||||||.||.||||| .||
GGAGGAGAGGAGGAACG-TAA

EST sequence (targeted fragment marked with yellow):
CTTAGTGCTGCTCAATGCAAGGAGGAGAGGAGGCTTGGGCTTAATGCCTGCAAGCCAGTTATTTATGGCAAGCTTCCATCACCAGCTTGCTGTGAGCGTGTAAGGGTTAGCCATATTGAATGTGTCTGCCCCGTTATCACACCAAAGCTGGCAGCTCTAATTGACCTCGATCGAGCCATTCGGCTGATTGAAGGTTGTGGTAGAAGGGTTCCTCGTCACTTCAAGTGTGGGAGTGTCACCACTCCATGAATTGAAGATTTAAATTTCTAACTAGTTCAATCCGGTCCTTTGTTCTTCTCTGTGACTGCAGAGGATTTATATACAGTCTATATAACTAGAATGAATAAAAGATTTTGAATACTTTATTATCCAAAAAAAAAAAAAAAAAACTCGAGGGGGGGCCGCGGTACCCCAATTCGCCC


gi|73942169|gb|DT525479.1|DT525479 WS02045.C21_H08 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02045_H08 3', 

CCTCCTCTTCTTCTTGCTGTT
 || |||||||||||| |.||
AGACGAGAAGAAGAACTATAA

EST sequence (targeted fragment marked with yellow):
GCCCCCTCGAAAGGCCTTGTGGTTTAGGGTTTTAACGTCTCTACAATCTACAGTTTTACTTGTCTGTGCCCCAAAAACCCTCGAGAGCTTCACAGCCATGGCGAGCACCAAAGTCCAGCGTATTATGACCCAACCCATTAACTTGATTTTCAGGTTTCTTCAAAGTAAAGCTCGCATTCAGATTTGGCTTTTTGAGCAGAAGGATTTGAGAATTGAAGGCCGAATTATTGGCTTTGATGAGTACATGAATTTGGTTTTGGAGGATGCTGAGGAAGTCAATATCAAGAAGAAGAGCAGAAAGTCTTTGGGAAGGATTCTTCTTAAGGGAGACAATATAACTCTGATGATGAACACGGGAAAGTAATGGTCATTCCTGGTTCTTTAAGAGGAGTTTGAGTGGAACAGCTTTGGGTAACAAACATTAGTAGGTTATGAATGAAAAAAATGTTCTTGTAATGAGGTGTGTCTTTACATTTGACTTGACAGTGTAACATCTCTATCGTAAGAATTGCTATTAATTATATTGGTGCTTGTCAGCGTTACCAATATTTTATCAAAAAAAAAAAAAAAAAA


gi|52519111|gb|CV266136.1|CV266136 WS02029.B21_D07 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02029_D07 3', 

CCTCCTCTTCTTCTTGCTGTT
|||| ||||||||.|||| .|
GGAGAAGAAGAAGGACGAGGA

EST sequence (targeted fragment marked with yellow):
TTCGATCAGTAGAGCTCTACTGAAATCCATCCCCACTTCAAACTACAACTACTGCTCAATCACCAGCTATGTTATTTCAACACCCATTTGAGGGAGTGGGTTGTCTTGTCTTCAGATCTCAAGTTGAGGAGTTGCTTATTTACAATGCTGGATCCATGTGAGAGACAGGGATCAGAGTCTGAAGAAATGAGTAGCAAATCACTAGAAACCGAGAGCCCACAGAAGAAGACTTGTGCTGATTGTGGCACTTCAAAGACTCCACTTTGGAGAGGTGGTCCAGCTGGTCCTAAGTCACTGTGTAATGCTTGTGGGATTAGGAGCAGGAAGAAGAAGAGGGAAATTTTAGGGTTGAATAAGGGAGGGGCAGCAGCAAATGACAAGAGAGCCAAGAAAGGGAGCAGTAACAATGGCTCAAGTAATTATAATAACAATAACAAGCAATTAGGTGATGGACTAAAGCAGAGATTACTGGCTCTGGGTAGAGAAGTCCTGGTGCAAGGATCAACGGTGGAGAGGCGAAGGAGGAAGCTAGGTGAAGAAGAGCAAGCTGCAGTGTTATTGATGGCCTTATCTTATGGCTCTGTTTATGCTTGAGTTGATGATTTTTGTGTAGGCGGGGGAGGTTAATAAGGCGATCAAGAATGTGTTAGCTAACTAAGAATGTGTGGGTTCGGGTTAATTTTTTTTAGTATCAGGTATTTTTTAGGTGTTTTGGGCTAACTGTACCGGAGATTTGTAGCAGTGTCGCAAGAGCTCAGAAAATGTTGAGACTTCATTCTATTTTTTGCAAAAAAAAAAAAAAAAAA


gi|52514547|gb|CV261572.1|CV261572 WS02017.B21_C14 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02017_C14 3', 

CCTCCTCTTCTTCTTGCTGTT
|||||||.|||||||  | ||
GGAGGAGGAGAAGAAGAAGAA

EST sequence (targeted fragment marked with yellow):
GCTTCCTTCCTTCCTTCCTTTTTAATTATTCGTTACTTACCAAAAACAAAAAAACCAAGAATCCAAATATATATATATATATATATATATATATATAGAGAGAGAGAGAGAGAGAGGGGAGGGGGTTGTTCCAATTAGAAAAAGAAGAAGAAGAGGAGGAGGAGGATGTCAATGAGAATAAAGGCGGTGGTGGACAAGTTCGTGCAGGAGCTGAAAGAAGCGTTGGATGCAGATATACAGGACAGAATCATGAAAGAAAGAGAGATGCAGAGTTACATCGAAGAACGTGAACGCGAAGTCGCCGAGCGTGAAGCTGCTTGGAAGGCGGAGCTCTCTCGCCGCGAGGCAGAGATTGCTCGCCAGGAAGCAAGGCTGAAGATGGAGAAAGAAAATCTCGAGAAAGAGAAAAGTGTTCTCATGGGAACCGCCTCCAATCAGGATAATCAAGATGGAGCTCTGGAAATCACTGTTAGTGGCGAGAAGTATCGATGCCTCAGATTCTCGAAGGCAAAGAAATGAGGGGCTTTATATAAGAAAATGTGTGAACAAGGGTCTCACCTTCATCCATTCCTCCCCATATTGTTAGCATAGCTACCCTCTGAGAAAAGAGAAAGAAAGCAGAGGACCCATCATAGATCGTGACTCTAATATGCAGTATTCTTTCCTTGCTCACTTGCTATCCGAGGAATGAATTTGGTTCTTTTCAAATCCATTAATTATGTATATCTGTACTACTTGAAGTGACAATGCTTCTTCTAGGCATGTGAATGAATTCAAAAAAAAAAAAAAAAAA


gi|73941822|gb|DT525132.1|DT525132 WS02044.C21_I13 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02044_I13 3', 

CCTCCTCTTCTTCTTGCTGTT
| |||||||||||.|| ||.|
GTAGGAGAAGAAGGACAACGA

EST sequence (targeted fragment marked with yellow):
GCAAGGCCCTATGGTCAAAGAGCTGTCCTCAAGTTTGCCCAGTACACTGGTGCTCATGCAATTGCTGGAAGGCATACCCCTGGTACCTTCACTAACCAGATGCAGACTTCATTCAGTGAACCTCGTCTCTTGATCCTCACTGATCCAAGGACTGATCACCAGCCTATCAAGGAAGCAGCTCTTGGAAACATTCCAACCATTGCTTTCTGTGACACTGACTCTCCCATGAACTTTGTGGATATTGGCATTCCTGCTAATAACAAAGGCAAGCACAGCATTGGATGCCTGTTTTGGCTGTTGGCAAGAATGGTTCTGCAGATGCGTGGAACTATTCCGCAAGGGCACAAATGGGATATTATGGTGGACTTGTTCTTTTATAGGGAACCTGAAGAAGCCAAGCAACAGGAAGAAGAGGATGCACTACCAGCTGCTGATTATGCTCTCCCTCCTCCTGATTATGGGATTTCTGCAGGTGAATGGGGTAGCACTATAGCTGATAGTCAATGGACTACTGATGTTGCCCAACAACCCATTCCTGCCGCCAATTTCTTCCCAGAACAAGGTGTTCTTTCTGGCGAATGGGGTGCAGCTCCGGCTCCGGCTCCAGCTCCAGCTCCTGCTCCTGCTGCTGAACAGCTCCCAGGTGCTGAACTTGGTGTGCCTCCTCCTGCTACTGCTGCTACTGGCTGGGACGTTTAAATTCAGCAAGAATGCTCATGTTTACAACTACCATCATTCTTGTTTACAATTACCATGTTTTGCACTCAGTGGAGAATCATGAGTTAGTTTTGTGGGGAATGGACTGTTTTTGTTTTTTTACATTTTTGGGCTCGGTGTAGTTCTAGACGGTTAACTATTTTTTTATATAAGATTTACACCGATTTTGGTATTTAAAGAAAAAAAAAAAAAAAAAA


gi|52520411|gb|CV267436.1|CV267436 WS02031.B21_L20 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02031_L20 3', 

CCTCCTCTTCTTCTTGCTGTT
|||||||||||| | ||||||
GGAGGAGAAGAA-ATCGACAA

EST sequence (targeted fragment marked with yellow):
AAGAACTTTGTTCGGCTTGATATTATCGATCATGGCTAAGAGTGATGTGAAGCTTATAGGGGCATGGCCGAGCCCTTTTGTGATGAGGGCAAGAATTGCCCTTAATATTAAATCTCTGGGGTATGAGTTTCTTGAGGAAAAACTGGGATCCAAAAGCCAGCTTCTTCTTGAATCAAACCCTGTCCATAAGAAAATTCCAGTTCTGATTCATGATGGCAAGCCCATTTGTGAATCTCTTGTGATAGTGGAGTACATTGATGAGGTTTGGTCTTCTGGACCTACCATCCTCCCCTCTGATCCTTATGATCGTGCCCTTGCTCGGTTTTGGGCTGCCTATCTCGATGAGAAGTGGTTTCCTTCGATGAGAAGCATTGCAACAGCTAAAGAAGAGGAGGCTCGAAAGGCGTTGATAGAGCAAGCAGGAGAAGGGGTGATGATGCTGGAGGATGCATTTAGCAGGTGTAGCAAAGGGAAGGGTTTCTTTGGAGGAGATCAGATCGGGTACCTTGACATTGCATTTGGTTCCTTTTTGGGGTGGTTGAGAACCACAGAGAAGATGAATGGAGTGAAGCTGATTGATGAAACTAAGACTCCTAGCTTGTTGAAATGGGCGACTAGTTTTTCTTCACATCCTGCTGTGAAGGATGTTCTTCCAGAGACAGAGAAGCTTGTTGAGTTTGCCAAGGTTCTGGCCAAATTCAAAGCAGCCAGCTCGAACTCCTAAATAACCATTATTGCCTTGTGGGCAACTTGAAATACTAGAAATTAAGGTTCTTATGTAATTTGAAATTGCTAGAAATTAAGCTTGTTGAGGAGAAATAAATTTAGATTACTTGCTGTGAACAATGATGGGGGCTCTAGTTACCATTTGCTTGTAATTTTGCTGTACCATTTTGCTTGAAAAAAAAAAAAAAAAAA


gi|52516925|gb|CV263950.1|CV263950 WS02023.B21_D13 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02023_D13 3', 

CCTCCTCTTCTTCTTGCTGTT
||||||||||||||| || .|
GGAGGAGAAGAAGAAGGAGGA

EST sequence (targeted fragment marked with yellow):
GCAAACACACAAGAAGAAGAGGAGGAAGAAGAAGAGGAGGAGGAAGACGAAGGTATACACTCTTTGATCACAACAAAGTAGATAGCAATGGAGGCCCGTGTTTGTTTGCTTGCAATGATGATCTTCTTTGGCTTCTTGGCATCACCAGGTGCTAAGACTTGCCACGGGAGAGATGTCGCCATCCAGTTCTACAGAACGGAGCTTTCAAGAATGGCCTTGCAGGTTGTCAAACGCGATTATTACAAAAGGAGAATTCCCAACCAAGTACTGCCTCCGGCCTCCAATTACATGCGCAATCAAGGTGCACCAACACCGTTACCACCACCAATAAACACTAGATCTTGAGATCAAATATTTTGTCTATGTTTCTGCCATTAATTCCCTTTAGAGGGGTTCAAGAGATCTCTAATGTTGAATTAAGTGAATAATCTGTGTCTTTTAATGTTAAATTTCAACATGCTGATTTTCGCTCCAAAAAAAAAAAAAAAAAA


gi|52514547|gb|CV261572.1|CV261572 WS02017.B21_C14 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02017_C14 3', 

CCTCCTCTTCTTCTTGCTGTT
||||||||||||||| || ||
GGAGGAGAAGAAGAA-GAAAA

EST sequence (targeted fragment marked with yellow):
GCTTCCTTCCTTCCTTCCTTTTTAATTATTCGTTACTTACCAAAAACAAAAAAACCAAGAATCCAAATATATATATATATATATATATATATATATAGAGAGAGAGAGAGAGAGAGGGGAGGGGGTTGTTCCAATTAGAAAAAGAAGAAGAAGAGGAGGAGGAGGATGTCAATGAGAATAAAGGCGGTGGTGGACAAGTTCGTGCAGGAGCTGAAAGAAGCGTTGGATGCAGATATACAGGACAGAATCATGAAAGAAAGAGAGATGCAGAGTTACATCGAAGAACGTGAACGCGAAGTCGCCGAGCGTGAAGCTGCTTGGAAGGCGGAGCTCTCTCGCCGCGAGGCAGAGATTGCTCGCCAGGAAGCAAGGCTGAAGATGGAGAAAGAAAATCTCGAGAAAGAGAAAAGTGTTCTCATGGGAACCGCCTCCAATCAGGATAATCAAGATGGAGCTCTGGAAATCACTGTTAGTGGCGAGAAGTATCGATGCCTCAGATTCTCGAAGGCAAAGAAATGAGGGGCTTTATATAAGAAAATGTGTGAACAAGGGTCTCACCTTCATCCATTCCTCCCCATATTGTTAGCATAGCTACCCTCTGAGAAAAGAGAAAGAAAGCAGAGGACCCATCATAGATCGTGACTCTAATATGCAGTATTCTTTCCTTGCTCACTTGCTATCCGAGGAATGAATTTGGTTCTTTTCAAATCCATTAATTATGTATATCTGTACTACTTGAAGTGACAATGCTTCTTCTAGGCATGTGAATGAATTCAAAAAAAAAAAAAAAAAA


gi|52522011|gb|CV269036.1|CV269036 WS0207.B21_C12 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS0207_C12 3', m 

CCTCCTCTTCTTCTTGCTGTT
|||| ||||||||||| ||||
GGAGCAGAAGAAGAAC-ACAA

EST sequence (targeted fragment marked with yellow):
ACTCCTCCTCGTGCTCAGTTCCTATATTTTTGTTTCTTAGCTTGTTCATGTCGAAATGAGAGACAGAATGGAAAGGCTTGTTCTTCTTCCTTTCTCCATTGGTTGCGTCTCTGAGTCAAGTGTGGCCATAGGCGTGCACCAAACTAAAAGAGCAAAGACAGACACAAACTTATCTGCGTCAAGAACACAAGAAGAAGACGAGGAAAGCTCATCAAGCACAGAAAGTACGAAAAATTCGTTGAAGTTGCTTGCTCTTTCAAAGCCTAACGTATCCACTGGGTTTAATAAGCTAGTCAAGGGTTTGAAGACCTTTCCTCAGTTATTTGCGTACAAAGAAGAGATGGAAGAATTGGAAGTGGAAATGGAAATTGGGTTGCCAACAAATGTTAAGCACGTGACACACATAGGGTGGGATGACTCACCAAATACTAACCCCGTTCAGGGCTGGGACAGTCTCATTTCTACCGATTTACTTTCCCTTCAATCTGCAACTTCAAGGCAGTTCGAGCTTGCCATAGCAGCAGGACAGGCTAATTCACCCCTTGTTAGGGCTTCCTCTGCTTAATCCAAACATGACTATAGAGATGACAGTGGATATTGTTGACCAATTGTGATGTTGGTGGTGGATATATATATGGATATATATATGTATATGTAAAAAAAAAAAAAAAAAA


gi|52512280|gb|CV259305.1|CV259305 WS02011.B21_A02 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02011_A02 3', 

CCTCCTCTTCTTCTTGCTGTT
 |||||||||||||| || ||
AGAGGAGAAGAAGAAGGAGAA

EST sequence (targeted fragment marked with yellow):
CAACGAGAAAGATCTCAGGGATTATGTTACTGGGTTTCATAAGAGGAAGAAGAAGAGGAGAAAGGAAGCAATTAAACAACAAGAGGTGAAGTTAAGGCACAAGCGTATCGCGGCACGCAAACAGAGGAAACTGGAAAAAGAACTTGCTTTAAATGGAGGAGCTCCACCAGCTGCTGATGAAAGCGATAACTATGGAGAAGATGATGAAGAAATTGAGCCAATTGCATCAATAAGTGGGACAACAAAGTACGACAATGGGGACATGCAAGTTACTGTGACCACCAGCGAGATTTCACGTGAAGACGAGGATGGCCATAGTGAAAAGACACAAGTGACAGTGCCAAGGTTGAGTGAAGCTGATAAATCGCACAAGTTATCCGTGAGCAAAAAGAATTCATTCAAGAAAGTTTTGAAACACAAATCACGGTCAAAGCCACAAAACAAAAGGGACAGAAAGAAGGGAAAGACGAATACCAAGAAGCGGTAGTTTATACTTTATAGTCTATCTTCTTGCGAATCATGGGTTCAAACTGTGCTTTCTATTATTAAAATACAGTCTAACAGAGGCCAATTGCTGAGACGTTATGTTAGTCCATGTAGAAAATTGGTTCTTTTCAGCATTTTGTATTGTGAAAGATATCAGAGATTTTTGGTGGCAGTTCTATCATATCAAAGATTTTTGGTGGCAAAAAAAAAAAAAAAAAA


gi|52513723|gb|CV260748.1|CV260748 WS02014.B21_O13 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02014_O13 3', 

CCTCCTCTTCTTCTTGCTGTT
|||||||||||||||||||||
GGAGGAGAAGAAGAACGACAA

EST sequence (targeted fragment marked with yellow):
GCAAAATTTTTATTTGTGTTTTCAATGGAAAATAACAATACTGGTGGTAGTGTTGATAATACATTCAGAAGAAAATTCGATCCAGAAGAGTACTTAGAACGCGCTCGTGAGCGTGAAAAACAGGAAGCCGAGGGTCGTGGGAAATCTAAATCGAAAGGGCCACCGGTGCAAAGGAAGCCCTTGAAGCATAGGGACTATGAAGTTGATCTCGACTCTCGGTTGGGGAAAACTCAGGTTGTTACACCAATTGCACCTCTAAGTCAGCAGGCTGGATACTACTGTTCAGTTTGTGAGTGTGTGGTAAAGGATTCTGCAAACTACTTGGATCACATCAATGGAAAGAAACACCAAAGAGCTTTGGGCATGTCTATGCGGGTGGAGCGTGCATCTCTTCAACAGGTCCAAGAGCGATTTGAAAAGCTCAAGAAACGTAGAGAACCTGGAAGCTTCACAGAGCAAGATCTTGATGAACGGATCTTAAAACAGCAAGAAGAAGAGGAGGAACGCAAGCGTCAACGCCGAGAAAGAAAGAAAGAGAAAAAGAAAGAGAAGGCAGCAGAGGAGGAAGAAGCTGATATTGATCCTGATATTGCAGCTATGATGGGGTTTGGCGGTTTCGGTTCATCAAAAAAATAATCATAATGTTTGTTCATACTTCTCTACCAGGAATTAGCTTTGCCATGATGTAACATCAGGAGCCTTCTTCATATGGAAATTCTGAGCTTTGCCGTGTAACATTTTGTAGTTTCCCTCTCTCAGGCTGTCTTTCATGCTCTCCTAGCAATCAAGTCTGTTAGAGGAAAAACAGCCACTCATTTGCTAGACATGTACTGTAGATTCAACGGTTATAATACATTTTCACTGTTTCATCTACCCTATTTTCTGTGACAAAAAAAAAAAAAAAAAA


gi|52523347|gb|CV270372.1|CV270372 WS0151.B21_N04 PTxN-IB-A-6 Populus trichocarpa x Populus nigra cDNA clone WS0151_N04 3', mRNA 

CCTCCTCTTCTTCTTGCTGTT
||.||||.||||||| ||.||
GGGGGAGGAGAAGAA-GATAA

EST sequence (targeted fragment marked with yellow):
TAATAAAAATCATGCATGGTTTTTTAAGTCGATGTAGTGGAATAGAAGAAGAGGAGGGGGGATCTGGGATTCTGCTAATTGCATGGATCATGTTGTTTAACTGTGGCCACCGGCCAGCTTTTTAAGTGGGAGATTCTGCGGTTTGGATTGTGCACTTAAGAGGTATATTTTGTAGAACTTTGCCTCTCATTTTGGATAAAGTTGGATGCTTGGTGTGTGTCCTTGGCTTGTTCAATTGAATGAGACTTGTATGCACCTGCTATTGTTAGGATTTCCTTGCCCTCTATCGAGCTGTGAAAAATAGGGCAAGAGGTTGTTGATATTGATAAGCACATGAAGTTTGCGCACATTGTTTTACTTGTCATGGCCATTGATCAGCATTATAGCTTAGTTGTACGAGGATTCACTTCAAATCTACCTATTGTTTCGTGTTGCGTCATAGGATCTGTTTCTTGGATTGATTTACCCGCTGTTATGTTTGGTTATATTGCTGCCAACATTAATAAGAATTATAAAGGCACCAAAAAAAAAAAAAAAAAA


gi|73938304|gb|DT521614.1|DT521614 WS02034.B21_M07 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02034_M07 3', 

CCTCCTCTTCTTCTTGCTGTT
||||||||.|||||.. |.|.
GGAGGAGAGGAAGAGTTATAG

EST sequence (targeted fragment marked with yellow):
GACTATATGTTTTTACTAGCAAAGCTAGTTATGCTTTTCTAGCCTGTTTCAAAAGTGCTGAATTGGGTTTGCTAAGAGAGAAGCTGGATGCCTTCCTACTGCTCATGATCTTCAAGCTTTGCCGCAGAATATCGACCTCTCTTGCCCTGCTGTATTCTTCGTTCTCCACAAAAACTAATCGTGCTTTCAGCTCCTTAATGCACCCCTCCTTGGCCTTCACTTCCATCTTTAGCTCCTCAATCATCTCTTCCTCTGTTGACCTCAGAGCACTTGCCAAGTACACACAGGGTTTCAGTGGTGCTGATATTACAGAAATATGCCAGCGTGCTTGCAAATATGCCATAAGAGAGAACATTGAGAAAGATATTGAGAAGGAGAGGAGGCAAAAAGAGAACCCTGAGGCTATGGAGGAAGATGTTGAGGATGAGGTGGCAGAGATCAAGGCAGCACATTTTGAGGAATCCATGAAATATGCCCGTCGGAGTGTTAGTGATGCTGACATCCGGAAGTATCAGGCATTTGCTCAAACACTGCAACAGTCAAGAGGGTTTGGAACTGAATTCAGGTTTGCTGAAGCTAGTGCTGGGTCCGATCCGTTTGCAGCTTCTGCTGGCGGAGCTGATGAAGATGACCTTTACAGTTAAGTTATCGTAAAATGTCTAGGCTTATAGATTTGGGCCCTAATATGTGTCTCTGGTTAAATCCATGCATGTTTACTGAAAGTTCCTTGGGATTTTATTATTGTGACCAAATTGGCCTTGCGTTTTTCAAAATTGAACACTTCCTTTGACTCCTTTCAAGTCAAAATTGAACATGTTATCTTATTTTGAAAGATTATTACTGATACTATCTGAAACTAAGTTTCCTCCAAAAAAAAAAAAAAAAAA


gi|73941825|gb|DT525135.1|DT525135 WS02044.C21_I16 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra cDNA clone WS02044_I16 3', 

CCTCCTCTTCTTCTTGCTGTT
|||| ||||||||||| .|.|
GGAGAAGAAGAAGAACAGCGA

EST sequence (targeted fragment marked with yellow):
GCCTGAGAAAAAAAGTCTTCCTCTCCCCTCTATATTAATCTCTCTTTTTCAAGAGTCCCAAATGGCACCCAAGGCCGAGAAGAAGCCGGCGGAGAAGAAGCCAGCCGCAGCAGAGAAAGCACCAGCGGAGAAGAAGCCAAGGGCAGAGAAGAAATTGCCCAAAGAAGGAGCTAGCGACAAGAAGAAGAAGAGGACCAAGAAGAATGTGGAGACTTACAAGATCTACATCTTCAAGGTCTTGAAACAAGTCCACCCTGATATTGGGATCTCAAGCAAAGCTATGGGTATCATGAACAGTTTCATCAATGATATCTTTGAGAAGCTTGCTCAAGAGTCTTCAAGGCTTGCTAGGTATAACAAGAAGCCCACCATTACCTCTCGGGAGATCCAGACTGCTGTCAGATTGGTTTTGCCTGGAGAGCTTGCTAAGCATGCTGTTTCTGAAGGGACTAAGGCTGTTACCAAGTTCACTAGCTCTTAGGTGGTTAGTGCTTGTTGGGCTTTTGTGGAATTAGGATTTATGTTTGATGTAAAGGTTGTTTAGGTCCTGATCTGAACAAAATCCATGTAGTTTCTTTTGTTCTAATCAATGAAGCTTAGTCTTAGCTCCAAAAAAAAAAAAAAAAAA