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mature miRNA: TGCATTTGCACCTGCATCTT
species: Populus x canadensis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|56827123|gb|CX179699.1|CX179699 E01_45-18_09.ab1 leaf inoculated with Marssonia pathogen of Populus euramericana Populus x ca 

TGCATTTGCACCTGCATCTT
.|| ||||||||| |||||
GCGAAAACGTGGAGGTAGAC

EST sequence (targeted fragment marked with yellow):
GGCACGAGGGTGGACAAGAAGGAAACAGGGGATGTGCTTTCCAATTCGGATGGTTCATCTGGAATGCAAATTACAGAAGCACTCAAGCTGCAGATGGAGGTGCAAAAGCGTCTACATGAGCAATTGGAGGTGCAGAGACAGCTACAATTACGCATAGAAGCCCAAGGGAAGTATTTGAAGAAGATAATTGAAGAGCAACAACGATTGAGCGGAGTTCTTGAAGATGTGCCTGGTTCAGGGGTCTCTGCCCCAGTATCAGGTGACAACTGCCCAGTATCTGACAACAAAACAGACCCAGCAACCCCTGCCCCAACTTCTGAATCACCCCTCCAAGACAAGGTTGCCAAGGAATGTGCCCCAACAAAGAGTCTTTCAATTGATGAATCATTCTCCTCTCAGCACGAGCCATTAACACCAGATTCACGTTGCAATACTGGTTCCCCAGCAGAGAGCCCTAGAGGTGAGAGGTCACTAAAGAAGCAAATGGTAAGCATGGGTGTAGCATTTGGCAAACCAGAGATGGTTCTTACACACCAGATATTGGAGTCGAGCTTAAATTCCTATCCACAACCGCACTCTGCCTTCTTGACCAGAGAGCAATTCGATCCTTCATCTGGGTTATCAATGGGAAACGAAGATCAATCGGAGGTCTTAGGCAGTGACCTTTATTTTACTGGCTGCATGATGTGTACCCT


gi|56828086|gb|CX180662.1|CX180662 C10_45-20_06.ab1 leaf inoculated with Marssonia pathogen of Populus euramericana Populus x ca 

TGCATTTGCACCTGCATCTT
||||.|||||||||||.||.
ACGTGAACGTGGACGTGGAG

EST sequence (targeted fragment marked with yellow):
GGCACGAGGGCCAGTCTCATTGTATATAATACTACTAGTTATAGAGAGTGAGTGACAGCTAGTAGCTAGCAGTTGTTTGATATAATATGATAAACAGGCTGCAGGTCATACAGGCATCTCATGCATGCCAATATGTTGCCTTTATCTGCATTTGCACCTGCATCTTTTGCGTTTGTTTTGTTTTTGACTCCACAAACAAAATCAAGTTCTGTCGATACAGTATGTGGGACAATTAGATATAGAGTGTTAGATATGGAAACTAATAAGCTTAGTTGAGGTGCAGGTGCAAGTGCAGGTGAATGCCATTTTGGTAATGTTTTCACTCACATATATATACGGCAGTGCTGCCTCTTTTCTTTTCATCTGTCTGTGGTTAATTTGCAGTTTCATGGTGGCAGAAATATTGTACTGGTAATCATCAAGGAGAAAGGGCAAGAAATTCTTGTGCTTATTAATTAACTATGAGAGCAAATTAATGTGTTTTCAACAACGACAGGCCATGTATAAAATTCTTTATGCAGGTGCTTGGTGTTTATAGAGGTGATGATGAATTGAAGTGATTGTGCAGCCACAATTCAATGGATGTGGAAGAATACTTCAAGGGTTTTCCAGTAATCTTTGAATTAAGTACTTGGAGGACAGATCATCCATGATCAATA