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mature miRNA: TTGAGGTGCAGGTGCAAGTGC
species: Populus x canadensis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|56832030|gb|CX184606.1|CX184606 F04_45-43_12.ab1 leaf inoculated with Marssonia pathogen of Populus euramericana Populus x ca 

TTGAGGTGCAGGTGCAAGTGC
.||||||||||.|||||| ||
GACTCCACGTCTACGTTCTCG

EST sequence (targeted fragment marked with yellow):
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCACGAGGTAACTTAATATTTAGATCGAGAAATACCCTTCGCAGCTCTTCATTTCCTCCACCCCTTGTGAAATTTCTTCCTCTTTTACTTTCTCCTAATTTACCGAGGTTGTCTGAAAATTTAGACGAGAAAGAAAGATGCCGGGATCGGAGACAGGAGTGATGACAAGCAGGGAGCCTTTTAGCGTGACAGGACTCCAACAAAAAACAGCGGTACAATCACAGCCGTTTATACAAAACATGCGTTTAGACTTCGGTACTGACGGTACTGCTGTTTACAAACCGATATCCACCGTCACTACCACTTCCGCGGTGTCACCTACCTACCCGCCTGGAGGAGGGGAAGGACCGGCTGGTGGGGCGGTGGTATCTCCTCACGGGATTGATGTGAATATGGGAGGAGGGATTGGAGGAGAGTCAATGAAAAGAAAGAGAGGGAGACCAAGGAAGTATGGGCCAGATGGCACTATGGCATTAGCTCTTGCATCTGCACCTCAGTCTGTTGCTGTAACCCAACCAACAAGTACAGCCACTGGAGGTGGTTTCTCTTCTCCTCCAGCTCAGACTCACCCTCTTGTTTCTCCTCATCCTCCTCCCCCACCTCCTGGATCTGATATTGGTGTCGTTGGTGCTGCTGGTGTTGTTTCTTCT


gi|56832798|gb|CX185374.1|CX185374 A05_45-53_01.ab1 leaf inoculated with Marssonia pathogen of Populus euramericana Populus x ca 

TTGAGGTGCAGGTGCAAGTGC
||||||||||||. || || |
AACTCCACGTCCGGGTCCAAG

EST sequence (targeted fragment marked with yellow):
AGTGGATCAAAGAATTCGGCACGACGCACGTCATTTCCAATTTTCTGGTTATTTCCGGCAGCAACGACGCGACGCATCAACTTCTTCTGAAAAGGAAGCAATGGATTTTTTGCTTGAGGGAACAAATGAGGACACATCTGAATGCCATTTTGATGACAAGGACATTCACAGATGCCCATTTTTGAGGAACATCAACAAGCCAACCAGCTTTTCGTTCTCTCCTGTGAATTTCCTTAATCCTGTACAAGGAGCTAAAGGTCCAATTTTTGAAGATGGTCCGAATTTTGGTATGGCATTTAAGCTCTTTCATGGCAAGGATGGGGTTGTCCCACTCTCAAATCAGTCCAGCTTTCATGATAACACTTTGGAACCTGGGCCTGCACCTCAATTCAATCCCTTAGCTGCAAAAGCTGCCACCATCAGTCTGTCCGCATTTGGTCCTGGTGGACCATTTGGCTTTGGTTCCTTCAATGATAAGTGGAAGAATCAGAAAAAGAAATCAGAATCAGCTAGCAAGAAAGAATCATCTTCTCAGAAAGGAAACACATCAAAACATGAGGCAATGGGAAACGAATGGTTGGAAACAGGCCACTGCCCCATAGCCAAGTCCTATAGAGCTGTGAGCCGTGTACTCCCACTAGTAGCATCGACTCT


gi|56828542|gb|CX181118.1|CX181118 C12_45-11_06.ab1 leaf inoculated with Marssonia pathogen of Populus euramericana Populus x ca 

TTGAGGTGCAGGTGCAAGTGC
||||||||||||. || || |
AACTCCACGTCCGGGTCCAAG

EST sequence (targeted fragment marked with yellow):
AACCAGCTTTTCGTTCTCTCCTGTGAATTTCCTTAATCCTGTACAACGAGCTAAAGGTCCAATTTTTGAACATGGTCCGAACTTTGGTATGGCGTTTAAGCTCTTTCATGGCAAGGATGGGGTTGTCCCACTCTCAAATCAGTCCAGCTTTCATGATAACACTTTGGAACCTGGGCCTGCACCTCAATTGAATCCCTTAGCTGCAAAAGCTGCCACCATCAGTCTG