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mature miRNA: TGAATCTTGATGATGCTACAC
species: Theobroma cacao
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|26055166|gb|CA798080.1|CA798080 Cac_BL_5361 Cac_BL (Bean and Leaf from Amelonardo type Cacao) Theobroma cacao cDNA clone Cac_

TGAATCTTGATGATGCTACAC
|||| ||||||.| | |||||
ACTTTGAACTATTTCCATGTG

EST sequence (targeted fragment marked with yellow):
GCAGGAATTCGGCACGAGGATAAGCTAAACTCCACTTTGTCTGAGGTTGAGTCAATTCTCAATGAGACAACAGTCCATAAGACAGTGGAAGAATCTGTTGATAACGACATAATTAAGAGCTCAAAAATGTCAGATGAGGGTGTACCTTTATCAAGTTTCAAAGAAGAGATAACTGAAGGTGGCGCAAGAAGTGGTAATGAAGTCAGGGTGCCAATGCTTAAAGGAGTAGAAATACAGTCAGGTGGCACAATTTTGGAAAGGAAGGAAATTGCAACAGCAGGATTTTTNNNTATNNCTCTGGTGCTGCTCTATCGCCAAATCCCACTAAGGCCTTCGCAGGTGGAGAGGATGCTNATNTTNATANTCTTTGCCACAAAATGGNCTTGNGTGTNAGCTNGAATGGAAGTNAGGTNCAGTNGGT



gi|223631745|gb|ES440361.1|ES440361 EST00579 Theobroma cacao-Moniliophthora perniciosa compatible interaction library Theobroma

TGAATCTTGATGATGCTACAC
|||||.|||||||||| ||
TCTTAGGACTACTACGACGTC

EST sequence (targeted fragment marked with yellow):
GTGCAAAGAGTTTGAGGGCAGAGAACCCTGCTGCTGCAACAGTGGGTGGTCCACCTTTCAAAGGACCAGTAGGGCCGTCAGATCAGCCTATTTTTTGGAATAGTGGATATCCTAGATTCTTTCCTAGCGAGGAAAGAGCAATGGTGAATCGAGTTGAATTAGGTCCTCCGCAAGGTCTCCCCGATTGGGCATGGCAAACGCATGGTCAGGTCAGTGTTACACCAATGCCACTGTTCTCTACTGCAGCATCATCAGGATTCTCGTTCTCGGCTACCCCTCGCTCTGCTGCCATTCTCCCG



gi|212120104|gb|CU511438.1|CU511438 CU511438 PODWB2S_KZ0ACN Theobroma cacao cDNA clone KZ0ACN1YB08FM1, mRNA sequence

TGAATCTTGATGATGCTACAC
|||||.|||||||||| ||
TCTTAGGACTACTACGACGTT

EST sequence (targeted fragment marked with yellow):
CGGGAGAGGTGTAACCTTGCATAAATGTGGCCGATGGGAAGCTCGAATGGGACAATTCCTGGGAAAGAAGGCCTATGACATGGCTGCAATGAAATGTAATGGAAGGGAAGCTGTAACCAACTTTGACCCAAGCACTTACGAAGGGGAAATTACAAATAGTCTAGGAGGCAGTTCTCACAATCTTGATCTGAGCCTTGGGATTTCTGCCCCTTCTGTCGCCCAAAATGCAAGCAGCAATGCAGGGGATTTCCATTTTCAGCATGCTGCTAGTGCCAGAGAGAGACCAGTGTTTGAGAGCTCTGCTCCTACATCTACTGGTGTAGAACCTCAACATGGTCCAACAATAGGATCTAAGCACCCTCCCATATTGTCTGGTGTTTATCCTGGCCTATTACCAAACAATGAGGAAAGGGGTATGGAGAAGAGGATCGAAGCTGTTTCCACGACAAGGTTTTCAAACTGGGCAAGGCAAATGCAAGGCAATGGCAACATTACGGCAACCCCAGTGATTCCTATTGCAGCATCATCAGGATTCTCTTCATCCAGTGCCACTGCATTATCAACTGCTTTCCCTTTCAGCAATTCTGTCCAAAACATCTGCCTTCTGACACCGGCCACATCCCCAAATAACAATTCTTATTACTTTAGTTACAGGAGCTGAAATCAAACAAGACCTCAAACCGCCACTGCTCTTCATTATGCTCTTTAGAAAAACTAGGTGAGCAAAAA



gi|211994218|gb|CU593076.1|CU593076 CU593076 SEED34I_KZ0AAH Theobroma cacao cDNA clone KZ0AAH1YI16, mRNA sequence

TGAATCTTGATGATGCTACAC
|||||||||||||||| ||
TCTTAGAACTACTACGACGTA

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTGGTTGTTGATGTTATGATTTCGAAGTGTGCAAAGTGGCACACTGTACTTTGAAACTGAGAGAAAGAAAGGGTCTTTTGTATAGTCGTTTATTGCCGATGCAGCATCATCAAGATTCTCACTTTCCAAACAACTTTGGCATAAAGGAGAAGGGAAAAGAAAGGGGAAGGGGAGAAAAAAAGAAGAGAGTTTGTATTATCCATCACCACCACCGTGCATTTGCATGTGAATCTTGATGATGCTACACCCGCAAACAACGACTGTCAATCTATCTATCCAATATCCATGCACAACATAAAGAGAGAGAAAGCCTTGCTGGAACTCAATATGGGATTTAGTATATAAAAGGAGAGGAGAGAAAGGCAAACATTTGAGTTTGACCCAAAGGGCCAAAGATCATGGGAAAAATTTTTGGAAGAAAAACAAAATAAAAATCCCG



gi|38499942|gb|CF974182.1|CF974182 PSU_meup2.1G07 Defense-related ESTs from Cacao Leaves Theobroma cacao cDNA, mRNA sequence

TGAATCTTGATGATGCTACAC
|||||.|||||||||| ||
TCTTAGGACTACTACGACGTC

EST sequence (targeted fragment marked with yellow):
TTTTCCAGGGGAAGCTCAAAATATCGAGGGGTGACGCTGCACAAATGTGGCCGATGGGAAGCACGGATGGGGCAATTCCTAGGCAAAAAGGCTTATGACAAGGCAGCTATCAAATGTAATGGGAGGGAGGCGGTCACCAACTTTGAGCCGAGGTCATATGAAGGGGAGATGACCTTTGATACCAGTAATGAAGATGCTCTTTATCCAGGCAATGATCACAACCTGGATCTGAACCTGGGGATATCTCCACCTGTTGGCAATGGTCCCAAGGAAAATGAGGGGCATCTCCATTTCCATACTGGTCCTTATGACGTGCATGGTGCAAAGAGTTTGAGGGCAGAGAACCCTGCTGCTGCAACAGTGGGTGGTCCACCTTTCAAAGGACCAGTAGGGCCGTCAGATCAGCCTATTTTTTGGAATAGTGGATATCCTAGATTCTTTCCTAGCGAGGAAAGAGCAATGGTGAATCGAGTTGAATTAGGTCCTCCGCAAGGTCTCCCCGATTGGGCATGGCAAACGCATGGTCAGGTCAGTGTTACACCAATGCCACTGNTCTCTACTGCAGCATCATCAGGATTCTCGNTCTCGGCTACCCCTCGCTCTGCTGNCATTCTCCCCGTCGAAACCTCTCGACCCAACNACCCCACAGTCTATGTTTTG



gi|223632242|gb|ES442408.1|ES442408 TSH_EST00906 Theobroma cacao-Moniliophthora perniciosa incompatible interaction library Theo

TGAATCTTGATGATGCTACAC
|||||.|||||||||| ||
CCTTAGGACTACTACGACGTC

EST sequence (targeted fragment marked with yellow):
AAATGCAGAGATATGGACAGTTCAGGAGACCTGGAGAGAATCATCTGTTTCACATTCTTCCTCCACACATTAATCCATCAACTTATCAGAGTCATTTTCCAAGCAGCAGCAATGGAAGCCGAGTTGGGAGTGATCTATCACTGTCTACTAATGATCAACAGTGGCAATCAGGTCCTCACCAATTGTTTGCAAATGCTGCAGCATCATCAGGATTCCCATCGCAGATTAGACCTTCCCAAACTTGGCTGCAGAAAAATGGGTTCCACTCACTCATGAGACCCTCCTGACCCATCTAAAAGTAGCCATAAAATCTCCGCCACTAATTAAAATCTCACCTTGTTGGTTTTGTAAGCATGTAAAGATCAATGCTTTGAGATTACTCCTTTTTTATCCCCCACGCCCCCTCTACAACTTTCTTAGAGAGCCAAAAGGAAAATTTACAAGAAATTATTG



gi|215530489|gb|CU580255.1|CU580255 CU580255 MONILIOU_KZ0ABV Theobroma cacao cDNA clone KZ0ABV3YB20FM1, mRNA sequence

TGAATCTTGATGATGCTACAC
|||||.|||||||||| ||
TCTTAGGACTACTACGACGTC

EST sequence (targeted fragment marked with yellow):
CGGGGGATGGGATGGCCTGCCAGTTGAGACAGGAGCAAGGATTGACAACTCTGCTCCGGCAACGATGAGGGTTCAACCTAGTCTTGGCCAGGCGATGGCATTCAAGCACCTTCAAAACTGGAATGGTGCAAATCCCAGTTTCTTTCCTGTTTATAGGGAAAGAGCAATAGAGAAGAGGATGGAAGTAGATTATGCACCAAACTGGGCATGGCAAATCCAAGGCCTTTACGGTGGGGCAAATCCAGTGCCACTCTTCCCTACTGCAGCATCATCAGGATTCTCGTCTTCAACAATTACTTCCCCATCAGCTGCTGCTGGCCAACTTTGCATTCCTAACACAACATTCCTCCACCGCCACCATTTCCCTCCGGCCATCACAAACCCGAACATCTCTCATTTCTACTGCCAGAGCTGAATAAGTTAGAATATGGCCACCATCTCGTATCTTCTCTACCGAGTAGAGAGTCTGGGTAGGCAATCAATGAAAATATTAGAAGGGGTTACAGGCTTACTGAAATTGATATAATGGAAGGCGGTACATAAAAGGGGAAATCAATTGATCGAGTTCATCATCGCATGTAAAAGCCTCATCAAACTAGTGTATTTGGTTTGATGATCATGATCAAAATTTGGGTCAATTTGAGCCCTTGGTTGAATGGAATGACTGTTATAGTTCCAAACCTTTGTTTCCATCATCTATTTTGTGGCCAGCAAGATATTTT