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mature miRNA: TGATTGAGCCGTGCCAATATC
species: Triphysaria pusilla
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|159582999|gb|EY134999.1|EY134999 CBTH474.g1_C23.ab1:P Triphysaria pusilla root tip normalized cDNA library Triphysaria pusill

TGATTGAGCCGTGCCAATATC
|||||||||||||||||||||
ACTAACTCGGCACGGTTATAG

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTTTTATGAAATTGATCAAATTATTGGCAACAGTGACGAAGTTTGAAATATATATCAGGGAGGCCTAAAAATCACCGAACAACTTAGACAACTTAATCGGGGGGCCGGCCTAATATATATAATTTTTTCGAACGAACTATGAATAAAAAATACACTATTTATATTTTTTCTTAACAAATTACAAATAAAAATACACTACTGCACAAAAAAATTGGGGGTGAGACCCCCCTGAACATGTAGAAGCTTCGCCCATGATTGGCAGGGCTTTCATTTCCCAAATAAGGCTATGAAGCTTATGATGCTTAGTTTCTTGCCTACAACTCTTGGATCTTCCAATTGAACAAGCAACTAAACATGTTTCCAACAACAATTATCATAAAATAATCACTTAATATTAAATGTAAAAGAATTTCCAGGATTTAGACTTTAAGAACATAATAATACTTAATCAATTATCATAAAATTGACGAGACGAGACGAAAAGCAAAGCGGAACATGATATTGGCACGGCTCAATCAAACTAAAGGACCCAATTCTTGGGAGATTTGATTTGATTGAGCCGTTCCAACATCATAACTAGCTACTACAAAAAGTGTGGCTTAGTTTTAATTAAAAAAAAAAAAAGTTTACCTTTATTGCAATCCTCACGTGGAAGTTTGAGTCGGATCATCAAGTCATTGCGATTTAGGATGTCTTGGTGAAGAGAA



gi|159582998|gb|EY134998.1|EY134998 CBTH474.b1_C23.ab1:P Triphysaria pusilla root tip normalized cDNA library Triphysaria pusill

TGATTGAGCCGTGCCAATATC
|||||||||||| ||||.|||
ACTAACTCGGCAAGGTTGTAG

EST sequence (targeted fragment marked with yellow):
AATGGAGAAAGTGACATTATTGTGCCAATTGATAAAATGTCATCAACCACTAATATATGTCTATAAACATCTAATGATAATATTTATATTATAAGAGTCCCAAGTTTTCAATCAGGCCAACCAACGACCAACATTTCCAACTACCAAAATCCTCTTTTTTCTTCCCTCCAAAATCCAAATGTACCCATTTTATTATTTTTCAATAAAAGGACATTTTATTAGGTTTCTCACTTTGTTCTATAATTAGGTATTCTCTTCACCAAGACATCCTAAATCGCAATGACTTGATGATCCGACTCAAACTTCCACGTGAGGATTGCAATAAAGGTAAACTTTTTTTTTTTTTAATTAAAACTAAGCCACACTTTTTGTAGTAGCTAGTTATGATGTTGGAACGGCTCAATCAAATCAAATCTCCCAAGAATTGGGTCCTTTAGTTTGATTGAGCCGTGCCAATATCATGTTCCGCTTTGCTTTTCGTCTCGTCTCGTCAATTTTATGATAATTGATTAAGTATTATTATGTTCTTAAAGTCTAAATCCTGGAAATTCTTTTACATTTAATATTAAGTGATTATTTTATGATAATTGTTGTTGGAAACATGTTTAGTTGCTTGTTCAATTGGAAGATCCAAGAGTTGTAGGCAAGAAACTAAGCATCATAAGCTTCATAGCCTTATTTGGGAAATGAA