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mature miRNA: GGAGCATCATCAAGATTCACA
species: Vitis shuttleworthii
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|51575591|gb|CV093427.1|CV093427 FAMU_USDA_FP_1450 Vitis shuttleworthii L., grape Vitis shuttleworthii cDNA clone JLVs079_B09 

GGAGCATCATCAAGATTCACA
| |||||||||||||||| ||
CGTCGTAGTAGTTCTAAGGGT

EST sequence (targeted fragment marked with yellow):
AGATACTATTAGCTTTTCTCTTCACATTCTCTCATCTTTTCAAGTTTTTGATTGTGGGTACATGTATAGAGTACCGTAGAATAGCTTTTCTACCAAGTGGGTCTTGTTTATTTCATAAGCTGAGATATGGTTTTGAAGCGTTCTGCTGTCCTTCCTTGCATTTGCCTTTACTTTTTCCTTCATAGAATGTGGTCTTGAGGTTAGGGCTAGGCTCCTTCTTCCTTTACATCTCTTTTTACTCGTTTTCTTTCTTTATTCCTTTACTTTTGCATGGATTCATATGATGAAAGAAGATCTCAAGACACCAGTCACTGTTTGCGGATGGAGCATCATCAAGATTCACAAGTATTGAGACTCAGTGCGTGGTGGTGATGGTGACTTTTGTGGTCCCTTCCACACTCCGATGGCTCTTTGATATGGGAATCTTGATGATGCTGCAGCGGCAATAAATGGCTAATTTTTATATAAATCATCTCCAAAACCACAAAACAGTTG