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mature miRNA: TAGCGAAGGATGACTTGCCTA
species: Vitis vinifera
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|110403058|gb|EC964155.1|EC964155 WIN087.C21_G15 Cab Sauv seed normalized (WIN08) Vitis vinifera cDNA clone WIN087_G15 3', mRN 

TAGCGAAGGATGACTTGCCTA
.||| ||||||||||| || |
GTCGGTTCCTACTGAAAGGTT

EST sequence (targeted fragment marked with yellow):
TCACAAGTTGATTGCAACCAATTGACTGCTCGCATTCCAATCACTGCCACCGAGATATACCATTGCGACTTACTAGCACCTGCTTATGGTACAAAGGCTATGATTCATCATCCTCAAATGATGGGAATGGCCCCTTCTCGAGTGCCATTGCCTGTACTAAATCCACAAGAAGAGGTTATTTTCATCAATCCAAAGCAGTATAATGGAATTATGAGGCGGAGGAAGCATCGCGCCAAGCTTGAAGCTCAAACCAACCCTGTCAAAGCTAGAAAGCCTTATCTCCATGAGTCTAGGCATCTCCATGCCTTGAAGAGGCCTCGTGGAGCTGGTGGACGCTTCCTCAACATGTCCAAGCTCCAAGAACCCAAGCCTTCTTCTCCCTCAACAGATGCTCTGATTGCAGGCTCAGCTCAGCCACCTTTCAATGGAAATACTGCATCAGAGTCTGAAGTTCATCAGCCAGAAAACAACAGAGAAGGGGCTTCCACCACCTCCTGCTCTGATGTCACTAGCGGCTCAAACAGTGATGACGTCTTTCTGCAGCCAGAATTCAGGTTCTCCACCTACCCTCCTCACATTGGCGGATCCATGAAAAGGAATGATTCTGGGAGCAATAACTTAGGTGGGTCTGGCCTCGGGTGACAGACGAGGCAGACAGTGGATGTGTCAGCAGCTTGTGTTCATCGTCCATCGAACGATCCGGCTGCTGGTGTCCCTTGGAAAGTCATCCTTGGCTGTGTTTTCTTCATTAGTTCATAGCTTTGTCAGCTGAATACAAGATACGGAGATTGTTACTAGCTGTTATTTCACTTTTTTTTTCCATTTTGTTTCTCTTCGGAAATACAAAAGTAG


gi|110383364|gb|EC947732.1|EC947732 WIN0542.C21_I13 Cab Sauv flower, leaf and root normalized (WIN05) Vitis vinifera cDNA clone 

TAGCGAAGGATGACTTGCCTA
 ||| |||||||| |||||.|
TTCGGTTCCTACTTAACGGGT

EST sequence (targeted fragment marked with yellow):
AGCACCTACCAGAGTCCCACTGCCCTTTGATATTGCAGAGGATGGACCTATTTTTGTCAATGCAAAACAGTATCATGGAATTCTCAGGAGGAGGCAGTCACGAGCAAAGATGGAGGCCCAGAACAAACTTGTCAAAGCCCGAAAGCCATATCTGCACGAGTCTCGGCATCTTCATGCCCTAAATAGGGTTAGAGGATCTGGTGGACGCTTCCTCAGCACGAAAAAGCTCCAAGAACCGGACTCAACTTCCAATGCTGGCTGTCATAGTGTATCTGGCTCTGGTCATTTTCACCAGAAGGGAGACACAACTGAGTATGAAGTCCATCAGTCTGATACTGGCAAATTTGTTGCTTCAACTACATCCAGCTCTGATGTCACCAGTGTCTCTAACAGTGATGTCCTTTTCAGGCAGCCGGAGCACAGGTTCTCAGGCATGTCTCCCCACATGGGTGGAGCCATGCAAGGTGGTGGCGGTGGGACTTATGGGCAATGGAGTCCTGCTCCTGGTTGTCCGGTGAGAAGTCGATAGGAACAAGATCGATGGAGTCACTGGTCTGGGCAATTCATCCTTGGCTTTGTTACTTTCGTTTCATGCGTGTTAAGAAGATAAACACATCAAACTTCATGGTGTAGTAGAAATACTCTGCCTTTCCCATTTCCAACTGCATACATTCTGGCTCTGTAAACATGGTTGAGAAGAGGCTATGCTTGAAACTCTCTGTTTGTGAACCATTGTTTTGTTTCTTCAAGACAATGTGAGATATTGGTTCACCGGTATTTTGTTTGTTGCTTACAGAAAGCAAACCCTGCCTTTTGTGCTAGTGTAGTGATGTTGATGCCAGTTTTTC


gi|110390919|gb|EC955863.1|EC955863 WIN0571.C21_P18 Cab Sauv flower, leaf and root normalized (WIN05) Vitis vinifera cDNA clone 

TAGCGAAGGATGACTTGCCTA
||||.||||||.| || ||.|
ATCGTTTCCTATTTAAAGGGT

EST sequence (targeted fragment marked with yellow):
AACATACATTGCCTCAGAACGGATCATTCCTGGATATGGTGGGGGCATGAGTTCAGCAAAGGCTTCTCTGGAGAGTGATACACGGGTACTGGCTTTTGAAGCTGGGAGAAAACACAAAATCAGAGTCAATACCATATCTGCAGGTCCATTAGGAAGTCGTGCTGCAAAAGCAATTGGATTTATTGACATGATGATCAACTACTCTTTGGCCAATGCGCCCTTACAGAAAGAACTACATGCAGAGGAGGTGGGGAATGCTGCTGCTTTTTTGGTGTCACCATTGGCTTCTGCTATCACTGGTGCTGTTGTGTATGTGGACAATGGTCTGAATGCAATGGGGGTGGGAGTCGACAGCCCAATATTTGAAAACCTTGACATTCCAAAAGATCACTAGAGGTAGCGGTAACAAGTGTGCTAAGTGAACCATATTCAAAATTTTGATTCTGCAAAAATAATCTTTGTTATGTATTTTGTGAGAATAGATCAGTTGGCGTATCCATTATAATATGAGGATATCTGATGGGAAATTTATCCTTTGCTATCATATGATGCTCTATCTGCAAAATACTTCACCTCATGTTGTTTGTAATAGCTGTTGTGCGATGTTAGCGGCGAGATTACTGTCCCTGAGCTTTGATTTATGTTTGAGTCCCCTTAGGGCCACTAGAGGTTTACCCGGTTGTTAACTTCAAGGTCCCATGGGATTAGTTGAGGTGTGCGTAAACTGGTCCGGACATCCACAGTTATATATAAAAAAAAAAGCTTTGATTTAAAAAAAAAAAAAAA