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mature miRNA: TGGTGCTTGGACGAATTTGCTA
species: Vitis vinifera
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|161705653|gb|FC056601.1|FC056601 sT7aVVM_AER53A10 VVM Vitis vinifera cDNA clone VVM_AER53A10 5', mRNA sequence 

TGGTGCTTGGACGAATTTGCTA
||||||||||| |||.|||  |
ACCACGAACCTACTTGAACACT

EST sequence (targeted fragment marked with yellow):
GGGGCATCTTCTTGAACTGAGGAGAAAGAGGGGTGGTCGAGGTTGTGGTTGTGGTTGATGCAGAAGAATTTTGATGATCTTCACAAGTTCATCCAAGCACCATCTAAAGCTCAAACAGGCCTACGATTTTCTTCAAAACTAGGCTTATTCATGATGGTGCTTGGACGAATTTGCTAAGAACATTAAGTTCTTGAAAGTAAAGCCAAAGGGGCAGCTCAGTTCTGCAAGGTTTTTTCTTCTATGTTGATGGATCCACCATAACTTTGCATGGTAGGTAGTTGCTTCTTGAAATAGAGGCCGGGAAGCTACCAGAGACACAGATCTCCACGCTGAATCCTTAAACCTGGAAGTTCTCTTTTGCTTCTTCAAACTATGGGGGTGCGGAAA


gi|110411058|gb|EC976758.1|EC976758 WIN1032.C21_H02 Muscat Hamburg post-veraison pericarp normalized (WIN10) Vitis vinifera cDNA 

TGGTGCTTGGACGAATTTGCTA
||||||||||||||||||||||
ACCACGAACCTGCTTAAACGAT

EST sequence (targeted fragment marked with yellow):
GTTTTAACCATCAATAATGTTTATGATTGATTTTAGGCCGGCACAGCCCACACTTTCATCCCTAGCAGATCCGAACGGTCAAGCAGTACCCTGCATCCACCCTTCCGATTAACATCGGTTCGATTCCACTCCACCTACTAGGGTTGGCCGATTGCCATACAAACTGAAAGCATGCAAAGTTATGGTGGATCCATCAACATAGAAGAAAAAACCTTGCAGAACTGAGCTGCCCCTTTGGCTTTACTTTCAAGAACTTAATGTTCTTAGCAAATTCGTCCAAGCACCATCATGAATAAGCCTAGTTTTGAAGAAAATCGTAGGCCTGTTTGAGCTTTAGATGGTGCTTGGATGAACTTGTGAAGATCATCAAAATTCTTCTGCATCAACCACAGCCACAACCACAACCTCAACCACCCCTCTCTTCTCCTCAGTTCA


gi|110400686|gb|EC967260.1|EC967260 WIN0824.C21_F18 Cab Sauv seed normalized (WIN08) Vitis vinifera cDNA clone WIN0824_F18 3', m 

TGGTGCTTGGACGAATTTGCTA
|| |||||||||.||||.| ||
ACGACGAACCTGTTTAAGCCAT

EST sequence (targeted fragment marked with yellow):
GGATGCTTTCAGACCTCAGAGTTTACCGAATTTGTCCAAGCAGCATAGCTTTCCCATGAATTCAGACCGGCATTATGGTCATGGAGGGGTCCCATGGGCTCGTAGCAGTGTTGCGGCCATTCCAATTTCATGCCCCATATGCTATGAAGATTTGGATTTCACGGACTCTAGTTTTTTACCTTGTTCTTGTGGGTTTCATCTTTGCCTTTTCTGCCACAAGAGGATTCTTGAAGAAGATGGGCGCTGTCCAGGTTGCAGGAAGCCATATGATTGTGATCCTGTTGAGGCAGAGGCCATTGTGAATGGGGGCAACCTGACATTCCGGTTGGGTCGCTCTTATAGCATGATTGCAAGGTCTTAGGAAGAGTCTTTATGTTTGATCAAACTATAGTATGTCTGGTCCATCCTCTTTCACATGTCTTGCGTGTTCAGTTACTCTTAGGTTCTTTTTTCGTAGAATTGGCAGTGGGTATAGGGTTGTCATACTAAAAGCACCCGGGAATTGAGCTGTTTCTCTTATGAGTTTGGTTCTTATTGATATGGTGCATTTGCTGTTATAGAGGATTCGTGTTGGTATGTGAATAGATAAGGTGGTATAGAAATTGAATACATCAAA