RetrogeneDB ID: retro_cjac_4113

Retrocopy location

• Organism: Marmoset (Callithrix jacchus)
• Coordinates: X:106103430..106104316(+)
• Located in intron of: ENSCJAG00000002447

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: PPP2CA
• Ensembl ID: ENSCJAG00000013634
• Aliases: None
• Description: protein phosphatase 2, catalytic subunit, alpha isozyme [Source:HGNC Symbol;Acc:9299]

Sequence

>retro_cjac_4113
GTCCAGCAGCTGAACGAGTGCAAGCAGCTGTCCAAGTCCCAGGTCAAGAGCCTCTGCAAGAAGGCTACAGAAATCCTGAC
AAAAGAATCCAACGTGTAAGAGATTTGATGTCCAGCCACTGTCTGTGGAGATGTGAATGGGCAATTTCATGATCTCATGG
AGCTGTTTAGAAGTGGTGGCAAATCACCAGATACAAATTCCTTGTTTATGGGAGATTGTGTTGACAGAGGATATTATTCA
GTTGAAACAGTTACACTGCTTGTAGCTCTTAACGTTCCTTACCATGAATGCATCACCATTCTGCAAGGGAATCATGAGAG
CAGACAGATCACACAAGTTTATTGTTTCTATGATGATTGTTTAAGAAAATATGGAAATGCAAATGTTTGGAATATTTTAC
AGATTTTTTGACTATCTTCCTCTCACTGCTTTGGTGGATAGACAGATCTTCTGTCTACATGGTGGTCTCTCGCCATATAT
AGATACACTGGATCATATCAGAGCACATGGTCACTTACAGGAAGTTCCCCATGAGGTTCCAATGTGTGATTTGCTGTGGT
CAGACCCGGATGACTGTGGTGATTGGAGTATATCTCCTTGAGGAGCTGATTACACCTTTGGGCAAGATATTTCTGAGACA
TTTAAGTATGCCAATGGCCTCACGTTGGTGTCTAGAGTTCACCAGCTACTGATGGAGGGATATAACTGGTGCGATGACTG
GAATGTAGTAATGATTTTCAGTGCTCCAAACTATTGTTATTATTGTGGTAACCAAGCTCCAATTATGGAACTTGACAATA
CTTTAAAATACTCTTTCTTAGAGTCTGACCCAGCACCTCATAGAGGCAAGCCACATGTTACTCATAGTACCCCAGACTAC
TTCCTG

Alignment

ORF - retro_cjac_4113 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 82.89 %
• Parental protein coverage: 95.79 %
• Number of stop codons detected: 3
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  IEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFM
          ..QLNECKQLS.SQVKSLC.KA.EILTKESNV.E..CP.TVCGDV.GQFHDLMELFR.GGKSPDTN.LFM
Retrocopy VQQLNECKQLSKSQVKSLCKKATEILTKESNV*EI*CPATVCGDVNGQFHDLMELFRSGGKSPDTNSLFM

Parental  GDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVW-KYFTDL-FD
          GD.VDRGYYSVETVTLLVAL.V.Y.E.ITIL.GNHESRQITQVY.FYD.CLRKYGNANVW..YFTD..FD
Retrocopy GDCVDRGYYSVETVTLLVALNVPYHECITILQGNHESRQITQVYCFYDDCLRKYGNANVW<EYFTDF<FD

Parental  YLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFG
          YLPLTALVD.QIFCLHGGLSP.IDTLDHIRA...LQEVPHE.PMCDLLWSDPDD.G.W.ISP.GA.YTFG
Retrocopy YLPLTALVDRQIFCLHGGLSPYIDTLDHIRAHGHLQEVPHEVPMCDLLWSDPDDCGDWSISP*GADYTFG

Parental  QDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDP
          QDISETF..ANGLTLVSR.HQL.MEGYNWC.D.NVV.IFSAPNYCY.CGNQA.IMELD.TLKYSFL..DP
Retrocopy QDISETFKYANGLTLVSRVHQLLMEGYNWCDDWNVVMIFSAPNYCYYCGNQAPIMELDNTLKYSFLESDP

Parental  APRRGEPHVTRRTPDYFL
          AP.RG.PHVT..TPDYFL
Retrocopy APHRGKPHVTHSTPDYFL

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP051959_colon	0.16 RPM	47.90 RPM
SRP051959_heart	0.93 RPM	54.08 RPM
SRP051959_kidney	1.44 RPM	40.98 RPM
SRP051959_liver	0.19 RPM	30.37 RPM
SRP051959_lung	1.33 RPM	40.75 RPM
SRP051959_lymph_node	0.14 RPM	43.29 RPM
SRP051959_skeletal_muscle	0.26 RPM	60.86 RPM
SRP051959_spleen	0.42 RPM	43.07 RPM

ChIP-Seq

Callithrix jacchus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_cjac_4113 retrocopy.

TSS

Callithrix jacchus was not studied using FANTOM5 data.

Lab

retro_cjac_4113 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_cjac_4113 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 9 parental genes, and 17 retrocopies.

Species	Parental gene accession	Retrocopies number
Anolis carolinensis	ENSACAG00000002653	1 retrocopy	retro_acar_69,
Callithrix jacchus	ENSCJAG00000012460	1 retrocopy	retro_cjac_985,
Callithrix jacchus	ENSCJAG00000013634	2 retrocopies	retro_cjac_1346, retro_cjac_4113 ,
Homo sapiens	ENSG00000113575	1 retrocopy	retro_hsap_369,
Nomascus leucogenys	ENSNLEG00000026729	1 retrocopy	retro_nleu_2441,
Otolemur garnettii	ENSOGAG00000009695	2 retrocopies	retro_ogar_3414, retro_ogar_921,
Rattus norvegicus	ENSRNOG00000005389	5 retrocopies	retro_rnor_1263, retro_rnor_1264, retro_rnor_1265, retro_rnor_1266, retro_rnor_817,
Sarcophilus harrisii	ENSSHAG00000016632	3 retrocopies	retro_shar_313, retro_shar_428, retro_shar_471,
Ictidomys tridecemlineatus	ENSSTOG00000010560	1 retrocopy	retro_itri_258,