RetrogeneDB ID: retro_acar_143

Retrocopy location

• Organism: Anole lizard (Anolis carolinensis)
• Coordinates: 5:128511102..128511549(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: GTSF1
• Ensembl ID: ENSACAG00000014260
• Aliases: None
• Description: gametocyte specific factor 1 [Source:HGNC Symbol;Acc:26565]

Sequence

>retro_acar_143
ACAGAGGACAACAATGTTAAAGATGTTGAAGCTTCAAATTCCGAGAAACTATTGCAGTGCCCATATGACAAAAGTCATCA
GATTAGGGCATGTAGGTTTCCCTATCACCTTGTCAAATGCCGTAAGCACCATCCTGGTATCATAAAAAGTTGGCCATGTG
CCCATTTAATGCCTGTCATCAGGTTTCCAGATAAGAGCTCTGCCAACACATAACAAATTGTGATGATAAAAACCTCATCG
AGCAGGACATCACATAGCAATAGAAGAGAAGTTAATGACGATTATGATGTCATACATTTTTCATGGCAACCTTGTCCATG
TGATGGAGACTGGGACAAAGAAGTGCTGGAGGACCCACCCCCAGCCTCTGCCTTCATTTGGGGCACCAGCTGCTCTGAAA
ACATATCTGTAATTAGATCATGTACAACTGTGGGAAACAAGAACCAC

Alignment

ORF - retro_acar_143 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 60.93 %
• Parental protein coverage: 82.94 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  TEDNYVDDLDPEN---LMQCPYDKNHQIRACRFPYHLVKCRKNHPDIV-QKLVMCPFNARHYVPREEISQ
          TEDN.V.D....N...L.QCPYDK.HQIRACRFPYHLVKCRK.HP.I...KL.MCPFNA.H.V.R.E..Q
Retrocopy TEDNNVKDVEASNSEKLLQCPYDKSHQIRACRFPYHLVKCRKHHPGII<KKLAMCPFNACHQVSR*ELCQ

Parental  HISHCDDKRSIEQDIA-NEQKNYRREVNVIH-SWQSPPCEEDWDKD-LED-SPATAFIWGTSRSVNRS--
          HI..CDDK..IEQDI.............VIH.SWQ..PC..DWDK..LED..PA.AFIWGTS.S.N.S..
Retrocopy HITNCDDKNLIEQDIT>SNRREVNDDYDVIHFSWQPCPCDGDWDKEVLEDPPPASAFIWGTSCSENISVI

Parental  ESSTVVGYKNH
          .S.T.VG.KNH
Retrocopy RSCTTVGNKNH

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP009831_adrenal	0.00 RPM	0.98 RPM
SRP009831_brain	0.00 RPM	1.28 RPM
SRP009831_dewlap	0.08 RPM	176.47 RPM
SRP009831_embryo	0.00 RPM	1.78 RPM
SRP009831_heart	0.00 RPM	1.19 RPM
SRP009831_liver	0.00 RPM	1.27 RPM
SRP009831_lung	0.00 RPM	1.51 RPM
SRP009831_ovary	0.00 RPM	173.31 RPM
SRP009831_skeletal_muscle	0.00 RPM	0.07 RPM

ChIP-Seq

Anolis carolinensis was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_acar_143 retrocopy.

TSS

Anolis carolinensis was not studied using FANTOM5 data.

Lab

retro_acar_143 was not experimentally validated.

Homology

Retrocopy orthology:

Anolis carolinensis does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 21 parental genes, and 48 retrocopies.

Species	Parental gene accession	Retrocopies number
Anolis carolinensis	ENSACAG00000014260	3 retrocopies	retro_acar_135, retro_acar_143 , retro_acar_92,
Anolis carolinensis	ENSACAG00000029590	1 retrocopy	retro_acar_141,
Ailuropoda melanoleuca	ENSAMEG00000000939	6 retrocopies	retro_amel_1420, retro_amel_324, retro_amel_338, retro_amel_639, retro_amel_724, retro_amel_796,
Bos taurus	ENSBTAG00000018085	1 retrocopy	retro_btau_1316,
Canis familiaris	ENSCAFG00000006465	2 retrocopies	retro_cfam_377, retro_cfam_592,
Choloepus hoffmanni	ENSCHOG00000001659	2 retrocopies	retro_chof_1261, retro_chof_950,
Callithrix jacchus	ENSCJAG00000018968	1 retrocopy	retro_cjac_904,
Dasypus novemcinctus	ENSDNOG00000001438	4 retrocopies	retro_dnov_2233, retro_dnov_2536, retro_dnov_256, retro_dnov_76,
Equus caballus	ENSECAG00000013618	2 retrocopies	retro_ecab_688, retro_ecab_806,
Echinops telfairi	ENSETEG00000009310	1 retrocopy	retro_etel_1619,
Felis catus	ENSFCAG00000010156	5 retrocopies	retro_fcat_1252, retro_fcat_1302, retro_fcat_1543, retro_fcat_557, retro_fcat_940,
Loxodonta africana	ENSLAFG00000016318	1 retrocopy	retro_lafr_1185,
Macropus eugenii	ENSMEUG00000016206	1 retrocopy	retro_meug_942,
Microcebus murinus	ENSMICG00000008444	1 retrocopy	retro_mmur_842,
Myotis lucifugus	ENSMLUG00000012961	3 retrocopies	retro_mluc_1177, retro_mluc_1286, retro_mluc_56,
Mustela putorius furo	ENSMPUG00000005669	3 retrocopies	retro_mputfur_1249, retro_mputfur_155, retro_mputfur_62,
Otolemur garnettii	ENSOGAG00000017227	2 retrocopies	retro_ogar_2617, retro_ogar_3251,
Rattus norvegicus	ENSRNOG00000036831	1 retrocopy	retro_rnor_2525,
Tarsius syrichta	ENSTSYG00000004679	2 retrocopies	retro_tsyr_1437, retro_tsyr_1880,
Tursiops truncatus	ENSTTRG00000004470	1 retrocopy	retro_ttru_249,
Vicugna pacos	ENSVPAG00000007016	5 retrocopies	retro_vpac_103, retro_vpac_203, retro_vpac_223, retro_vpac_490, retro_vpac_95,