RetrogeneDB ID: retro_fcat_326

Retrocopy location

• Organism: Cat (Felis catus)
• Coordinates: A2:68048832..68049619(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: MEST
• Ensembl ID: ENSFCAG00000022984
• Aliases: None
• Description: mesoderm specific transcript [Source:HGNC Symbol;Acc:7028]

Sequence

>retro_fcat_326
CTATCTGCATATCCCATACCTCCCTACCCGCAGTGTTCTCCCTCTCTTCACTCATGGCAGTTATCATGCAAATTTTTTAC
CTACAAGGGACTAGGCATCTTCCACCAAGGTTCTGTGGGTGTGGTTGGAAGTCTGCAATAGTTGTGCTTTCACATGGCTT
TCCAACATCCAGCTATGGTTGGCACAAGGTTTGGGATGGCCTGACCCTGAGGTTTCATCCAGTGATTGCCCTTGATGTCC
TATGCTTTGGCTTCGGTGACAAACTGAGGCCATATCACTATTCCATATTTGAACGGGCTGGCATTGTGGAGGCACCTCTG
TGGCATCTGGAGCTCTCAAACCACAAGATCAACCTTTTGTCTCATGATTACGGAGATATCGTTGCTTGGGGGCTGCTCTA
TAGGTTCAAGCAGAATTGATCTGGGAAGCTTGCCATAAACAGTCTCTGTCTCTCGAAGGGATGTGTACTTCCCAAGACTC
ACCAACTCACCTTCTCCAAAAGTTTCTCAAAGATGGAAACATGCTGTCGTCTATCCTCACGTGATTGATGAACCTTCTCC
TGAGGTCTCACACCCCGTCTTCGGGCCATGTGCCTGACACTCTGAGAGTGAGCCGTGGGACATGTAGGCAGGGATATAGA
ACGATGATGGCAATTCAGTTATCGACAGTCTCTTACAGGATTTCAGCCAGAGAAGGAAGTTTAGAAGATGCTGGGTGGGA
GCTCTTACTTCTGTAACAGTTGCCATTTACTTTTCTCGACGGGCCATTGGATCATGTGAATCCCTAT

Alignment

ORF - retro_fcat_326 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 68.4 %
• Parental protein coverage: 81.29 %
• Number of stop codons detected: 5
• Number of frameshifts detected: 4

Retrocopy - Parental Gene Alignment:

Parental  LAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQDSVGVVGSPE-IVVLLHGFPTSSYDWYKIWEGLT
          L.AY...P.PQ.SP.LHSW..S.KFFTYKGL.IF.Q.SVGVVGS...IVVL.HGFPTSSY.W.K.W.GLT
Retrocopy LSAYPIPPYPQCSPSLHSWQLSCKFFTYKGLGIFHQGSVGVVGSLQ<IVVLSHGFPTSSYGWHKVWDGLT

Parental  LRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRFKQN
          LRFH.VIALD.L.FGF.DK.RP.HYSIFE.A.IVEA.L.HL.L.N..INLLSHDYGDIVA..LLYRFKQN
Retrocopy LRFHPVIALDVLCFGFGDKLRPYHYSIFERAGIVEAPLWHLELSNHKINLLSHDYGDIVAWGLLYRFKQN

Parental  RSGRLTIKSLCLSNGGIFPETHR-PLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTP-VFGPYTRPSESE
          .SG.L.I.SLCLS.G...P.TH....LLQK.LKDG..LS.ILT.LMN.....R..TP.VFGP....SESE
Retrocopy *SGKLAINSLCLSKGCVLPKTHQ<THLLQKFLKDGNMLSSILT*LMNLLL--RSHTP<VFGPCA*HSESE

Parental  LWDMWAGIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVTI-PIHFIYGPLDPVNPY
          .WDM.AGI.N.DGN.VIDSLLQ...QR.KFRR.WVGAL.SVT..P..F..GPLD.VNPY
Retrocopy PWDM*AGI*NDDGNSVIDSLLQDFSQRRKFRRCWVGALTSVTV>PFTFLDGPLDHVNPY

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP017611_brain	0.00 RPM	38.10 RPM
SRP017611_kidney	0.00 RPM	7.01 RPM
SRP017611_liver	0.00 RPM	2.63 RPM

ChIP-Seq

Felis catus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_fcat_326 retrocopy.

TSS

Felis catus was not studied using FANTOM5 data.

Lab

retro_fcat_326 was not experimentally validated.

Homology

Retrocopy orthology:

Felis catus does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 15 parental genes, and 35 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000005659	2 retrocopies	retro_amel_1177, retro_amel_1874,
Callithrix jacchus	ENSCJAG00000010760	2 retrocopies	retro_cjac_1563, retro_cjac_3931,
Felis catus	ENSFCAG00000022984	5 retrocopies	retro_fcat_1067, retro_fcat_1645, retro_fcat_1693, retro_fcat_2004, retro_fcat_326 ,
Loxodonta africana	ENSLAFG00000006066	3 retrocopies	retro_lafr_578, retro_lafr_584, retro_lafr_748,
Macropus eugenii	ENSMEUG00000008227	1 retrocopy	retro_meug_885,
Microcebus murinus	ENSMICG00000006129	1 retrocopy	retro_mmur_1091,
Macaca mulatta	ENSMMUG00000009499	2 retrocopies	retro_mmul_1936, retro_mmul_94,
Nomascus leucogenys	ENSNLEG00000012841	3 retrocopies	retro_nleu_1065, retro_nleu_515, retro_nleu_790,
Otolemur garnettii	ENSOGAG00000009224	1 retrocopy	retro_ogar_3464,
Pongo abelii	ENSPPYG00000018006	4 retrocopies	retro_pabe_2337, retro_pabe_2510, retro_pabe_2511, retro_pabe_866,
Pan troglodytes	ENSPTRG00000019698	2 retrocopies	retro_ptro_1769, retro_ptro_2045,
Sorex araneus	ENSSARG00000000502	1 retrocopy	retro_sara_352,
Ictidomys tridecemlineatus	ENSSTOG00000020977	2 retrocopies	retro_itri_1210, retro_itri_1610,
Tupaia belangeri	ENSTBEG00000008404	2 retrocopies	retro_tbel_1480, retro_tbel_526,
Tursiops truncatus	ENSTTRG00000004934	4 retrocopies	retro_ttru_1026, retro_ttru_1115, retro_ttru_1133, retro_ttru_1907,