RetrogeneDB ID: retro_ptro_1465

Retrocopy location

• Organism: Chimpanzee (Pan troglodytes)
• Coordinates: 21:13547594..13548515(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: EIF4A1
• Ensembl ID: ENSPTRG00000008694
• Aliases: None
• Description: Pan troglodytes eukaryotic translation initiation factor 4A, isoform 1 (EIF4A1), mRNA. [Source:RefSeq mRNA;Acc:NM_001098575]

Sequence

>retro_ptro_1465
GATTCCCCATCCTGAGACAATGACCCTGATGGCATGGAGCCCAAAGGCATCATCGAGAGTAACTAGAATGAGATGGTTGA
CAGCTTTGATGACATGAAACTGTAGGAGTTTCTTCTCCGTGGCATCTATGCCTATGTTTTGAGAAACCCTCTGCCATCCA
GCAGCGAGCCATTCTACCTGTGTCAAGGGTTATGATGTGATCACTCAAGCCCAACCTGGGACTGGGGAAATGGCCACACT
TGTCATATTGATTCTGCCACAGATTGAATTAGATCTAACAGCCACCCAGGCCTTGGTCTTAGCACCCACTAGAGAACTGG
CTCAGCAGATACAGAAGGTGGTCATAACACCTGGAGACTACATGAGTACCTCTTGTCATGCCTGTATTGGGGGCACCAAT
GTGTGTACTGAGGTGCAGAAATTGCAGATGGAAGCTCCCCATGTCATCATGCATACCCCTGGATGCATGTTTGATATGGT
TCACTGGAGATACCTGTCTCCCAAATACATCAAAATGCTTGTACTGGACAAAGTTGATGAAATGTTAAGCCATGGATTCA
AGGACCAGATCTATGACATATTCCAAAGTTGAACAGCAACACCCAGGTAGTTTGCTGTCAGCTACAATGCTTTCTTATGT
GCTTGAGGTGACCAAGAAGTTCATGAGGGACCCCATTTGGATTCTGGTCAAGAAGGAAGAGTTGATCCTGGAGAGTATCC
ACCAATTCTGCATCAATGTGGAACAAGAGAAGTGGAAGCTGGACACACTGCGTGACTTGTATGAAACTCTGACCATCACC
CAGGCAAGTCATCTTCACCAACACTCGAAGGAAGGTGGGCTGACTCACTGAGAAGATGCATGCTTGGGTTTTCACTGTCT
CTGCTGTGCATGGAGATACGGACCAACAGAAATGAGATGTG

Alignment

ORF - retro_ptro_1465 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 79.23 %
• Parental protein coverage: 75.86 %
• Number of stop codons detected: 5
• Number of frameshifts detected: 5

Retrocopy - Parental Gene Alignment:

Parental  DSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYG-FEKPSAIQQRAILP-CIKGYDVI
          DS.S.DN.PDGMEP.G.IESN.NE.VDSFDDM.L.E.LLRGIYAY..FEKPSAIQQRAILP.C.KGYDVI
Retrocopy DSPS*DNDPDGMEPKGIIESN*NEMVDSFDDMKL*EFLLRGIYAYV<FEKPSAIQQRAILP<CVKGYDVI

Parental  AQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAE
          .QAQ.GTG..AT..I.IL.QIELDL.ATQALVLAPTRELAQQIQKVV...GDYM..SCHACIGGTNV..E
Retrocopy TQAQPGTGEMATLVILILPQIELDLTATQALVLAPTRELAQQIQKVVITPGDYMSTSCHACIGGTNVCTE

Parental  VQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQ-KLNSNTQVV-L
          VQKLQMEAPH.I..TPG..FDM...RYLSPKYIKM.VLD..DEMLS.GFKDQIYDIFQ.KLNSNTQVV.L
Retrocopy VQKLQMEAPHVIMHTPGCMFDMVHWRYLSPKYIKMLVLDKVDEMLSHGFKDQIYDIFQ<KLNSNTQVV<L

Parental  LSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQA-VIFIN
          LSATM.S.VLEVTKKFMRDPI.ILVKKEEL.LE.I.QF.INVE.E.WKLDTL.DLYETLTITQA.VIF.N
Retrocopy LSATMLSYVLEVTKKFMRDPIWILVKKEELILESIHQFCINVEQEKWKLDTLRDLYETLTITQA>VIFTN

Parental  TRRKVDWLTEKMHARDFTVSAMHGDMDQKERDV
          TRRKV..LTEKMHA..FTVSA.HGD.DQ...DV
Retrocopy TRRKVG*LTEKMHAWVFTVSAVHGDTDQQK*DV

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain_prefrontal_cortex	0.00 RPM	108.23 RPM
SRP007412_cerebellum	0.00 RPM	114.43 RPM
SRP007412_heart	0.00 RPM	90.88 RPM
SRP007412_kidney	0.00 RPM	190.54 RPM
SRP007412_liver	0.00 RPM	366.10 RPM
SRP007412_testis	0.00 RPM	114.55 RPM

ChIP-Seq

Pan troglodytes was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ptro_1465 retrocopy.

TSS

Pan troglodytes was not studied using FANTOM5 data.

Lab

retro_ptro_1465 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ptro_1465 has 3 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_2520
Gorilla gorilla	retro_ggor_1572
Callithrix jacchus	retro_cjac_2046

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Parental genes homology:

Parental genes homology involve 16 parental genes, and 66 retrocopies.

Species	Parental gene accession	Retrocopies number
Cavia porcellus	ENSCPOG00000004900	1 retrocopy	retro_cpor_1088,
Erinaceus europaeus	ENSEEUG00000003581	1 retrocopy	retro_eeur_610,
Felis catus	ENSFCAG00000024897	1 retrocopy	retro_fcat_370,
Homo sapiens	ENSG00000161960	10 retrocopies	retro_hsap_1004, retro_hsap_1182, retro_hsap_1252, retro_hsap_1270, retro_hsap_1796, retro_hsap_2520, retro_hsap_4836, retro_hsap_4873, retro_hsap_4931, retro_hsap_610,
Gorilla gorilla	ENSGGOG00000024702	8 retrocopies	retro_ggor_1321, retro_ggor_1572, retro_ggor_3015, retro_ggor_537, retro_ggor_798, retro_ggor_920, retro_ggor_983, retro_ggor_998,
Macropus eugenii	ENSMEUG00000010780	2 retrocopies	retro_meug_158, retro_meug_179,
Myotis lucifugus	ENSMLUG00000003415	3 retrocopies	retro_mluc_1047, retro_mluc_1229, retro_mluc_1805,
Macaca mulatta	ENSMMUG00000013499	1 retrocopy	retro_mmul_1280,
Monodelphis domestica	ENSMODG00000007090	3 retrocopies	retro_mdom_1282, retro_mdom_326, retro_mdom_711,
Mus musculus	ENSMUSG00000059796	7 retrocopies	retro_mmus_1810, retro_mmus_1853, retro_mmus_2320, retro_mmus_2473, retro_mmus_3122, retro_mmus_325, retro_mmus_527,
Nomascus leucogenys	ENSNLEG00000009547	7 retrocopies	retro_nleu_2109, retro_nleu_2117, retro_nleu_230, retro_nleu_2470, retro_nleu_2769, retro_nleu_440, retro_nleu_701,
Pan troglodytes	ENSPTRG00000008694	10 retrocopies	retro_ptro_1201, retro_ptro_1465 , retro_ptro_2954, retro_ptro_3112, retro_ptro_3247, retro_ptro_450, retro_ptro_750, retro_ptro_810, retro_ptro_862, retro_ptro_870,
Pan troglodytes	ENSPTRG00000015718	1 retrocopy	retro_ptro_437,
Rattus norvegicus	ENSRNOG00000030628	3 retrocopies	retro_rnor_1059, retro_rnor_2213, retro_rnor_2306,
Sorex araneus	ENSSARG00000006017	7 retrocopies	retro_sara_224, retro_sara_383, retro_sara_461, retro_sara_567, retro_sara_6, retro_sara_605, retro_sara_89,
Ictidomys tridecemlineatus	ENSSTOG00000024814	1 retrocopy	retro_itri_610,