RetrogeneDB ID: retro_mmus_3326

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 9:31567881..31568177(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Dhfr
• Ensembl ID: ENSMUSG00000021707
• Aliases: Dhfr, 8430436I03Rik, AA607882, AI662710, AW555094
• Description: dihydrofolate reductase [Source:MGI Symbol;Acc:MGI:94890]

Sequence

>retro_mmus_3326
AGAGTGACCACAATTTCTTCAATGGAAAGTAAACATGACCTGGTGATTTGGGGTGGGAAGCCTGGCTCTCCATTCTTGAG
AAGACTGAAGCTTTGAAGGAAAGAATTAACATAGTTCTCTGTAGAGAACTCGAGGAACCACCACAAGGAGCTCGTTTTCT
TGCCAAAAGTCTAGACAATGTCTTAAGACTCACTGAAAAACCAGAATTAGCAGATAAATTAGACGTGGTTTGGGCAGTGC
GAGGTGGTTCTGTTTACAAGGGAGTGATGAATCAGCCCGGTCACCTCAGATTGTGG

Alignment

ORF - retro_mmus_3326 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 66. %
• Parental protein coverage: 52.94 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  RMTTTSSVEGKQNLVIMGRK-TWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPE
          R.TT.SS.E.K..LVI.G.K..W.SI.EK...LK.RINIVL.REL.EPP.GA.FLAKSLD..LRL.E.PE
Retrocopy RVTTISSMESKHDLVIWGGK<AWLSILEKTEALKERINIVLCRELEEPPQGARFLAKSLDNVLRLTEKPE

Parental  LASKVDMVWIVGGSSVYQEAMNQPGHLRLF
          LA.K.D.VW.V.G.SVY...MNQPGHLRL.
Retrocopy LADKLDVVWAVRGGSVYKGVMNQPGHLRLW

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	4.02 RPM
SRP007412_cerebellum	0.00 RPM	4.21 RPM
SRP007412_heart	0.00 RPM	2.37 RPM
SRP007412_kidney	0.00 RPM	35.09 RPM
SRP007412_liver	0.00 RPM	27.85 RPM
SRP007412_testis	0.00 RPM	3.57 RPM

ChIP-Seq

RNA Polymerase II actvity near the 5' end of retro_mmus_3326 was not detected

EST

No EST(s) were mapped for retro_mmus_3326 retrocopy.

TSS

No TSS is located nearby retro_mmus_3326 retrocopy 5' end.

Lab

retro_mmus_3326 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_3326 has 0 orthologous retrocopies within eutheria group .

---

Parental genes homology:

Parental genes homology involve 19 parental genes, and 52 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000015750	2 retrocopies	retro_amel_218, retro_amel_842,
Bos taurus	ENSBTAG00000007681	1 retrocopy	retro_btau_351,
Callithrix jacchus	ENSCJAG00000019556	4 retrocopies	retro_cjac_2017, retro_cjac_2298, retro_cjac_2446, retro_cjac_531,
Equus caballus	ENSECAG00000026827	1 retrocopy	retro_ecab_513,
Felis catus	ENSFCAG00000026745	3 retrocopies	retro_fcat_579, retro_fcat_705, retro_fcat_841,
Homo sapiens	ENSG00000228716	4 retrocopies	retro_hsap_1927, retro_hsap_2119, retro_hsap_3602, retro_hsap_6,
Gorilla gorilla	ENSGGOG00000022366	2 retrocopies	retro_ggor_1959, retro_ggor_2429,
Myotis lucifugus	ENSMLUG00000012200	2 retrocopies	retro_mluc_1109, retro_mluc_2577,
Macaca mulatta	ENSMMUG00000018283	13 retrocopies	retro_mmul_1496, retro_mmul_425, retro_mmul_692, retro_mmul_693, retro_mmul_695, retro_mmul_700, retro_mmul_701, retro_mmul_704, retro_mmul_738, retro_mmul_760, retro_mmul_768, retro_mmul_82, retro_mmul_994,
Monodelphis domestica	ENSMODG00000024860	3 retrocopies	retro_mdom_1685, retro_mdom_604, retro_mdom_703,
Mustela putorius furo	ENSMPUG00000008704	1 retrocopy	retro_mputfur_1107,
Mus musculus	ENSMUSG00000021707	1 retrocopy	retro_mmus_3326 ,
Nomascus leucogenys	ENSNLEG00000012989	3 retrocopies	retro_nleu_1193, retro_nleu_2314, retro_nleu_2631,
Oryctolagus cuniculus	ENSOCUG00000005127	3 retrocopies	retro_ocun_1351, retro_ocun_1640, retro_ocun_650,
Otolemur garnettii	ENSOGAG00000025320	1 retrocopy	retro_ogar_222,
Pongo abelii	ENSPPYG00000015600	2 retrocopies	retro_pabe_2959, retro_pabe_41,
Pan troglodytes	ENSPTRG00000017042	3 retrocopies	retro_ptro_109, retro_ptro_1551, retro_ptro_2988,
Rattus norvegicus	ENSRNOG00000013521	1 retrocopy	retro_rnor_2030,
Sus scrofa	ENSSSCG00000014125	2 retrocopies	retro_sscr_104, retro_sscr_1131,