RetrogeneDB ID: retro_ecab_186

Retrocopy location

• Organism: Horse (Equus caballus)
• Coordinates: 1:127830816..127831277(-)
• Located in intron of: ENSECAG00000024689

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: RPS16
• Ensembl ID: ENSECAG00000004311
• Aliases: None
• Description: ribosomal protein S16 [Source:HGNC Symbol;Acc:10396]

Sequence

>retro_ecab_186
CCACACAGTAAGGAGCTCTTGTCTACGCCAGCATCCGTGCTGTCCAAGGGCCCTCTGCAGTCCATGCATGTCTTTGAACA
GAAGACGGCCACAGCCCTAGTGCATTGCAAACAGGGCAACGGCCTCATCAAGGTGAATGGGTGGCCCCTGGAGATGATGG
AGCTACGCACCCTGCAGTACAAGCTCCTGGAACCTGTTCTGGGCAAGGAGTGATTTAGCAGTGTGGACATCGTAGTCCGT
GTGAAAGATGTTTGTCACATGGCCCAGATTTATGCAATCCTCCAGTCCATCTCCAGAGCCCTAGTCCCCGTTACCAGAAA
TACGTGGATGAGGCTTCCAAGAAGGACATCAAAGACATCCTCATCCAGTATGATCCGGCCCTGTTGGTAGCTGATCCCCG
TAACTGCGAATGCAAAGAGTTTACAGGTCCTGGTGCCAGTGCCCACTATCAGAAAGCCTAC

Alignment

ORF - retro_ecab_186 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 71.52 %
• Parental protein coverage: 98.12 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  PRGEEQSSTPPAMPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLL
          P...E..STP....SKGPLQS..VF...KTATA..HCK.GNGLIKVNG.PLEM.E.RTLQYKLLEPVL..
Retrocopy PHSKELLSTPASVLSKGPLQSMHVF-EQKTATALVHCKQGNGLIKVNGWPLEMMELRTLQYKLLEPVL--

Parental  GKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAY-YQKYVDEASKKEIKDILIQYDRTLLVADPRRC
          GKE.F..VDI.VRVK...H.AQIYAI.QSIS.ALV...YQKYVDEASKK.IKDILIQYD..LLVADPR.C
Retrocopy GKE*FSSVDIVVRVKDVCHMAQIYAILQSISRALVPV<YQKYVDEASKKDIKDILIQYDPALLVADPRNC

Parental  ESKKFGGPGARARYQKSY
          E.K.F.GPGA.A.YQK.Y
Retrocopy ECKEFTGPGASAHYQKAY

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP021940_articular_cartilage	0.06 RPM	686.34 RPM
SRP021940_cerebellum	0.00 RPM	211.26 RPM
SRP021940_embryo	0.13 RPM	424.76 RPM
SRP021940_placental_villous	0.14 RPM	303.92 RPM
SRP021940_synovial_membrane	0.14 RPM	544.98 RPM
SRP021940_testis	0.00 RPM	192.34 RPM

ChIP-Seq

Equus caballus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ecab_186 retrocopy.

TSS

Equus caballus was not studied using FANTOM5 data.

Lab

retro_ecab_186 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ecab_186 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 19 parental genes, and 78 retrocopies.

Species	Parental gene accession	Retrocopies number
Anolis carolinensis	ENSACAG00000014176	1 retrocopy	retro_acar_194,
Bos taurus	ENSBTAG00000021093	3 retrocopies	retro_btau_1509, retro_btau_167, retro_btau_441,
Canis familiaris	ENSCAFG00000005501	6 retrocopies	retro_cfam_1349, retro_cfam_1754, retro_cfam_2142, retro_cfam_2147, retro_cfam_799, retro_cfam_908,
Callithrix jacchus	ENSCJAG00000014170	6 retrocopies	retro_cjac_2641, retro_cjac_2737, retro_cjac_3019, retro_cjac_885, retro_cjac_905, retro_cjac_949,
Cavia porcellus	ENSCPOG00000011359	3 retrocopies	retro_cpor_1266, retro_cpor_1408, retro_cpor_1515,
Equus caballus	ENSECAG00000004311	2 retrocopies	retro_ecab_186 , retro_ecab_779,
Felis catus	ENSFCAG00000010743	4 retrocopies	retro_fcat_1258, retro_fcat_1709, retro_fcat_1806, retro_fcat_1976,
Gorilla gorilla	ENSGGOG00000014414	10 retrocopies	retro_ggor_110, retro_ggor_1250, retro_ggor_1344, retro_ggor_1664, retro_ggor_1766, retro_ggor_193, retro_ggor_2423, retro_ggor_2819, retro_ggor_652, retro_ggor_667,
Loxodonta africana	ENSLAFG00000001541	1 retrocopy	retro_lafr_1191,
Macaca mulatta	ENSMMUG00000019370	8 retrocopies	retro_mmul_1018, retro_mmul_1080, retro_mmul_1175, retro_mmul_1219, retro_mmul_1403, retro_mmul_20, retro_mmul_767, retro_mmul_862,
Monodelphis domestica	ENSMODG00000013545	4 retrocopies	retro_mdom_1668, retro_mdom_1823, retro_mdom_815, retro_mdom_856,
Nomascus leucogenys	ENSNLEG00000014388	3 retrocopies	retro_nleu_1266, retro_nleu_248, retro_nleu_46,
Oryctolagus cuniculus	ENSOCUG00000002150	14 retrocopies	retro_ocun_1028, retro_ocun_1073, retro_ocun_1394, retro_ocun_1430, retro_ocun_1436, retro_ocun_1774, retro_ocun_371, retro_ocun_580, retro_ocun_632, retro_ocun_645, retro_ocun_670, retro_ocun_837, retro_ocun_906, retro_ocun_986,
Otolemur garnettii	ENSOGAG00000004497	4 retrocopies	retro_ogar_1229, retro_ogar_1309, retro_ogar_1313, retro_ogar_98,
Oreochromis niloticus	ENSONIG00000000006	1 retrocopy	retro_onil_52,
Pelodiscus sinensis	ENSPSIG00000016930	1 retrocopy	retro_psin_55,
Rattus norvegicus	ENSRNOG00000019578	3 retrocopies	retro_rnor_10, retro_rnor_1823, retro_rnor_302,
Sus scrofa	ENSSSCG00000010712	1 retrocopy	retro_sscr_731,
Sus scrofa	ENSSSCG00000020817	3 retrocopies	retro_sscr_478, retro_sscr_5, retro_sscr_723,