RetrogeneDB ID: retro_ecab_2

Retrocopy location

• Organism: Horse (Equus caballus)
• Coordinates: X:71435901..71437050(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSECAG00000003452
• Aliases: None
• Status: KNOWN_PROTEIN_CODING

Parental gene information

• Parental gene symbol: PABPC4
• Ensembl ID: ENSECAG00000009396
• Aliases: None
• Description: poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:HGNC Symbol;Acc:8557]

Sequence

>retro_ecab_2
ATGGGGAGTGGGGAGCCTAATCCTGCTGGCAAGAAGAAGAAGTACCTCAAGGCTGCCCTGTACGTGGGTGACTTGGACCC
AGATGTCACCGAGGACATGTTATATAAAAAGTTCAGGCCTGCTGGCCCTCTGCGCTTCACCCGAATCTGCCGTGACCCGG
TGACCCGCAGCCCCCTGGGCTATGGCTATGTCAACTTTCGCTTTCCTGCGGATGCTGAGTGGGCTCTGAACACCATGAAT
TTTGATTTGATTAATGGCAAACCATTCCGCCTCATGTGGTCTCAGCCAGATGACCGCTTAAGAAAGTCTGGGGTTGGAAA
TATATTCATCAAAAATCTGGACAAATCCATAGACAATAGGGCCCTTTTTTATTTATTTTCTGCTTTTGGGAACATTCTCT
CCTGCAAAGTCGTATGTGATGACAACGGCTCTAAAGGTTATGCCTATGTGCACTTTGACAGTCTGGCGGCTGCCAATAGG
GCCATCTGGCATATGAATGGAGTGCGGCTCAACAACCGCCAGGTGTATGTTGGCCGATTCAAATTTCCGGAAGAGCGGGC
AGCTGAAGTCAGAACCAGAGATAGAGCAACTTTCACCAATGTTTTCGTTAAAAATTTTGGAGATGACATGGATGACGAAA
AACTGAAGGAAATTTTCAGTGAATATGGGTCAACTGAGAGTGTGAAGGTAATAAGAGATGCCAGTGGGAAATCTAAAGGC
TTTGGATTTGTGAGATATGAGACACATGAGGCTGCCCAAAAGGCTGTGTTAGACCTGCATGGAAAGTCCATCGATGGAAA
AGTCCTATATGTAGGGCGAGCACAGAAGAAAATTGAACGTCTGGCTGAGTTAAGGCGAAGATTTGAACGGCTGAGATTAA
AGGAAAAAAGTCGGGCTCCGGGAGTGCCTATCTATATTAAGAACCTGGATGAGACCATTGATGATGAAAAACTGAAGGAG
GAATTTTCTTCCTTTGGATCAATTAGCCGGGCCAAAGTGATGGTGGAAGTGGGGCAAGGCAAAGGGTTTGGTGTTGTCTG
CTTCTCCTCTTTCGAGGAGGCTACCAAAGCAGTGGATGAGATGAATGGTCGCATAATGGGCTCCAAGCCCCTGCATGTCA
CCCTGGGCCAGGCCAGGCGTAGGTGGTGA

Alignment

ORF - retro_ecab_2 Open Reading Frame is conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 67.4 %
• Parental protein coverage: 55.15 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  YPMASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVI
          Y..A.LYVGDL..DVTE.MLY.KF.PAGP....R.CRD..TR..LGY.YVNF..PADAE.AL.TMNFD.I
Retrocopy YLKAALYVGDLDPDVTEDMLYKKFRPAGPLRFTRICRDPVTRSPLGYGYVNFRFPADAEWALNTMNFDLI

Parental  KGKPIRIMWSQRDPSLRKSGVGNVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYAFVHFET
          .GKP.R.MWSQ.D..LRKSGVGN.FIKNLDKSIDN.AL...FSAFGNILSCKVVCD.NGSKGYA.VHF..
Retrocopy NGKPFRLMWSQPDDRLRKSGVGNIFIKNLDKSIDNRALFYLFSAFGNILSCKVVCDDNGSKGYAYVHFDS

Parental  QEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKE-FTNVYIKNFGEEVDDESLKELFSQFGK
          ..AA..AI..MNG..LN.R.V.VGRFK...ER.AE........FTNV..KNFG...DDE.LKE.FS..G.
Retrocopy LAAANRAIWHMNGVRLNNRQVYVGRFKFPEERAAEVRTRDRATFTNVFVKNFGDDMDDEKLKEIFSEYGS

Parental  TLSVKVMRDPSGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKVIFVGRAQKKVERQAELKRKFEQLKQE
          T.SVKV.RD.SGKSKGFGFV.YE.HE.A.KAV....GK.I.GKV..VGRAQKK.ER.AEL.R.FE.L...
Retrocopy TESVKVIRDASGKSKGFGFVRYETHEAAQKAVLDLHGKSIDGKVLYVGRAQKKIERLAELRRRFERLRLK

Parental  RISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSITSAKVMLEDGRSKGFGFVCFSSPEEATKAVTEMNGR
          ..SR..GV..YIKNLD.TIDDEKL..EFS.FGSI..AKVM.E.G..KGFG.VCFSS.EEATKAV.EMNGR
Retrocopy EKSRAPGVPIYIKNLDETIDDEKLKEEFSSFGSISRAKVMVEVGQGKGFGVVCFSSFEEATKAVDEMNGR

Parental  IVGSKPLYVALAQRK
          I.GSKPL.V.L.Q..
Retrocopy IMGSKPLHVTLGQAR

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP021940_articular_cartilage	0.18 RPM	87.72 RPM
SRP021940_cerebellum	2.39 RPM	38.79 RPM
SRP021940_embryo	4.17 RPM	154.79 RPM
SRP021940_placental_villous	0.53 RPM	56.96 RPM
SRP021940_synovial_membrane	1.18 RPM	102.07 RPM
SRP021940_testis	0.58 RPM	77.63 RPM

ChIP-Seq

Equus caballus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ecab_2 retrocopy.

TSS

Equus caballus was not studied using FANTOM5 data.

Lab

retro_ecab_2 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ecab_2 has 1 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Rattus norvegicus	retro_rnor_92

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Parental genes homology:

Parental genes homology involve 16 parental genes, and 39 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000011535	2 retrocopies	retro_amel_107, retro_amel_676,
Bos taurus	ENSBTAG00000017125	3 retrocopies	retro_btau_1492, retro_btau_28, retro_btau_656,
Canis familiaris	ENSCAFG00000003081	2 retrocopies	retro_cfam_45, retro_cfam_837,
Choloepus hoffmanni	ENSCHOG00000005327	3 retrocopies	retro_chof_1150, retro_chof_1182, retro_chof_1274,
Equus caballus	ENSECAG00000009396	2 retrocopies	retro_ecab_2 , retro_ecab_502,
Felis catus	ENSFCAG00000015254	2 retrocopies	retro_fcat_116, retro_fcat_545,
Loxodonta africana	ENSLAFG00000000151	3 retrocopies	retro_lafr_1144, retro_lafr_390, retro_lafr_597,
Microcebus murinus	ENSMICG00000016633	1 retrocopy	retro_mmur_675,
Myotis lucifugus	ENSMLUG00000012068	2 retrocopies	retro_mluc_228, retro_mluc_397,
Monodelphis domestica	ENSMODG00000017657	2 retrocopies	retro_mdom_1090, retro_mdom_1805,
Mustela putorius furo	ENSMPUG00000014414	2 retrocopies	retro_mputfur_152, retro_mputfur_546,
Mus musculus	ENSMUSG00000011257	7 retrocopies	retro_mmus_2006, retro_mmus_2326, retro_mmus_2327, retro_mmus_798, retro_mmus_801, retro_mmus_803, retro_mmus_805,
Rattus norvegicus	ENSRNOG00000015642	2 retrocopies	retro_rnor_175, retro_rnor_92,
Sarcophilus harrisii	ENSSHAG00000018139	1 retrocopy	retro_shar_823,
Sus scrofa	ENSSSCG00000003660	3 retrocopies	retro_sscr_1210, retro_sscr_974, retro_sscr_975,
Tursiops truncatus	ENSTTRG00000014340	2 retrocopies	retro_ttru_1117, retro_ttru_246,