RetrogeneDB ID: retro_dnov_174

Retrocopy location

• Organism: Armadillo (Dasypus novemcinctus)
• Coordinates: GeneScaffold_2332:17940..18954(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: ZSCAN4
• Ensembl ID: ENSDNOG00000011483
• Aliases: None
• Description: zinc finger and SCAN domain containing 4 [Source:HGNC Symbol;Acc:23709]

Sequence

>retro_dnov_174
ATGGCTTTAGATGTTAGAACCTCCTTTTGCCATGACCCATCCAGGAGTGATCATCCTGGGTCAGACAATCTAGAGTTTGA
ATCCCCCCAAGGACCTGATGTCCAGAGGAGAGAGGGTAACTCTAATTTCCAGGACCCTCAACTCAGCTTATATCAAACTA
ACAATGACTTATTTGCAAAGCAGGAACTAAAAAGACTCCGTAAGTTATTTCACTTGTGGCTGCATCCAGAAAAACACAGC
AAAGAAGAAATTATTTTTCAATTGGTCCTGAAACAGCTTATGATGAATGGATACTGCAGGAACAAGTCCACTTTGAGAGA
GAAATGGGATTCAAGTAGAAGAAACCTGGAGAAACTCATGGAAGAGCTGACTGATGAATGCGTGAAGTCACCTGGTTTTG
TCCATGCCCTCATGCAGGGACAGGAAGCCCTCTTCTCTGAGAATATACCTTAAGAGAAGTCATTCATCTCCTGAAACAAC
TGCCAGCAGGAATCCCAAGAGGCGAGAGCATGAGGACACCCTTCCAAGGTTCCCAAGGTATTATTCTCCTTCAAACAGGA
CATGGAGATGAACAGAAAGACAGCAGGAACATGTCTATGAAAAGCAATCAAGTAAATGACAGTATTACTAGTTGAGGGAA
GAAAGTATCTTCCCTGGTTTTTATTCAGGAAGATGATAGCCCTAAGCCGGAAGAGGGGAATAGTTCTTGGGAGAACCTTT
GCAACTCTAGAAGAGCAGAGCTTGACTCCTCCACATCCAGGAAGGATCCCTAGAAGAATCCTCTTCTCAAGATATCCCTA
TGGAAGTAGAACCACATTTTTTCCCAGGACAGATCAAGCCAAGCATAAGCCTACCAACTAAAGCATTGATGGAACATCCA
GATGTGGGATAAACGAAGAAAGAGTCCATAGTCCCCCAAAATCATACAGGTGTGAGAACTGTCCCAGGATCTTTAGGTAT
CTCTCTCAGCGAGATGCCCACCAGAGAAGACACAGGAGTGAGAGACCATTTGTT

Alignment

ORF - retro_dnov_174 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 74.71 %
• Parental protein coverage: 78.52 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 3

Retrocopy - Parental Gene Alignment:

Parental  MALDLRTSFRHDPSRSGHPGSHNLELEPTQGCDLQRREGNSKFQGPQLSLYQTSNGSFAKQELKRLRKVF
          MALD.RTSF.HDPSRS.HPGS.NLE.E..QG.D.QRREGNS.FQ.PQLSLYQT.N..FAKQELKRLRK.F
Retrocopy MALDVRTSFCHDPSRSDHPGSDNLEFESPQGPDVQRREGNSNFQDPQLSLYQTNNDLFAKQELKRLRKLF

Parental  HLWLQPEKHSKEEIISQLILEQLVMNGYCRNRSILKEKWDSSGRNLEKLVGELNDDCMKSPDFVHVHMQG
          HLWL.PEKHSKEEII.QL.L.QL.MNGYCRN.S.L.EKWDSS.RNLEKL..EL.D.C.KSP.FVH..MQG
Retrocopy HLWLHPEKHSKEEIIFQLVLKQLMMNGYCRNKSTLREKWDSSRRNLEKLMEELTDECVKSPGFVHALMQG

Parental  QEALFSENMP-LREVIYLMEQMPAGTPRGESMRTPFQGPQN-IPLQTGHGDEYKDDSNIFVKRNQINVNI
          QEALFSEN.P.LREVI.L..Q.PAG.PRGESMRTPFQG.Q..I.LQTGHGDE.KD..N...K.NQ.N..I
Retrocopy QEALFSENIP<LREVIHLLKQLPAGIPRGESMRTPFQGSQGIILLQTGHGDEQKDSRNMSMKSNQVNDSI

Parental  TSQGNQVPYVVIIQEDDSPKPEEGSGSWKNLHNFRRAELGSPTS-QEGSLGESSSQDVPMEVE-TQFLPR
          TS.G..V...V.IQEDDSPKPEEG..SW.NL.N.RRAEL.S.TS.QEGSL.ESSSQD.PMEVE.T.F.PR
Retrocopy TS*GKKVSSLVFIQEDDSPKPEEGNSSWENLCNSRRAELDSSTS<QEGSLEESSSQDIPMEVE<TTFFPR

Parental  TDQASPKPALTNQSSDGTSRCGINQERVHSPPKSYKCEDCPRIFRFPSQLEAHQRRHRNERPFV
          TDQA..KP..TN.S.DGTSRCGIN.ERVHSPPKSY.CE.CPRIFR..SQ..AHQRRHR.ERPFV
Retrocopy TDQAKHKP--TN*SIDGTSRCGINEERVHSPPKSYRCENCPRIFRYLSQRDAHQRRHRSERPFV

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012922_ascending_colon	0.00 RPM	0.00 RPM
SRP012922_cerebellum	0.00 RPM	0.14 RPM
SRP012922_heart	0.00 RPM	0.00 RPM
SRP012922_kidney	0.00 RPM	0.00 RPM
SRP012922_liver	0.00 RPM	0.00 RPM
SRP012922_lung	0.00 RPM	0.00 RPM
SRP012922_quadricep_muscle	0.00 RPM	0.00 RPM
SRP012922_spleen	0.00 RPM	0.00 RPM

ChIP-Seq

Dasypus novemcinctus was not studied using ChIP-Seq data.

EST

Dasypus novemcinctus was not studied using EST data.

TSS

Dasypus novemcinctus was not studied using FANTOM5 data.

Lab

retro_dnov_174 was not experimentally validated.

Homology

Retrocopy orthology:

Dasypus novemcinctus does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 5 parental genes, and 16 retrocopies.

Species	Parental gene accession	Retrocopies number
Cavia porcellus	ENSCPOG00000005419	2 retrocopies	retro_cpor_1470, retro_cpor_285,
Dasypus novemcinctus	ENSDNOG00000011483	11 retrocopies	retro_dnov_1048, retro_dnov_1253, retro_dnov_1287, retro_dnov_1505, retro_dnov_174 , retro_dnov_1780, retro_dnov_2499, retro_dnov_2556, retro_dnov_2666, retro_dnov_859, retro_dnov_964,
Equus caballus	ENSECAG00000015200	1 retrocopy	retro_ecab_78,
Loxodonta africana	ENSLAFG00000003257	1 retrocopy	retro_lafr_171,
Ictidomys tridecemlineatus	ENSSTOG00000023779	1 retrocopy	retro_itri_408,