RetrogeneDB ID: retro_ptro_1061

Retrocopy location

• Organism: Chimpanzee (Pan troglodytes)
• Coordinates: 15:55322044..55322846(-)
• Located in intron of: ENSPTRG00000007113

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: CNN3
• Ensembl ID: ENSPTRG00000000979
• Aliases: CNN3, calponin-3
• Description: None

Sequence

>retro_ptro_1061
TATGGGCTGTCAGCCGAGGTCAAGAACTGGCTCCTGCCCAAATATGACCCCCAGAAGGAGGCAGAGCTCCGCAGCTGGAT
CAAGGGACTCACCGGCATCTCCACTGACCCTGACTTCCAGAAGAGCCTGAAGGACGGGATTATCTTATGCATACTCATGA
ACAAGCTGCAGCCGGCCTCAGTCCCCAAGATCAACCACTCCATGCAGAAGTGGCACCAGCTGGAAAACCTCTCCAACTTC
GTCAAGGCTGTAGTCAGCTATGACATGAACCCCATGGACCTGTTCGAGGCCAACAACCTATTTGAGAGTGGGAACATGAG
GCACATGCAGGTGTCTCTTTTTGCCCTAGCAGGGAAGGTCAAGACTAAGAGGCCACAGAGCGGGGTGGACATTGGCATCA
AGTACTCAGAGAAGCAGGAGCAGAACTTCAGTGACGCCGCCATGAAGGCTGGCCAGTGCGTCATCGGGCTGCAAATGGGC
ACCAACAAATGTACCAGCCAGTTGGGCATGACCGTGTATGGCGTGAGGAGGCATCTGTGTGACCCCAAGAATCACATCCT
GCCCCCCCATGGACCACTCGACCATTGGCCTCCAGATGGGTACAAATAAGTGTGCCAGCCAGGTGAGCATGACAGCTCCT
GGGACGTGGCAGCACATCTATGACACCAAGCTGGGAACCGACAAGTGTGATAATTCCTCCATGTCCCTGCAGATGGGCTA
CACCCAGGGTACCAACCATAGTAGCCAGGTCTTTGGCCTGGGCCAGCATATATGTGACCCCAAGTACTGCCTGCCAGGCA
CA

Alignment

ORF - retro_ptro_1061 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 57.25 %
• Parental protein coverage: 81.46 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  YGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIGTNFQLGLKDGIILCELINKLQPGSVKKVNESSLN
          YGLSAEVKN....KYD.Q.E..LR.WI...TG.S....FQ..LKDGIILC.L.NKLQP.SV.K.N.S...
Retrocopy YGLSAEVKNWLLPKYDPQKEAELRSWIKGLTGISTDPDFQKSLKDGIILCILMNKLQPASVPKINHSMQK

Parental  WPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQT
          W.QLEN..NF.KA...Y.M.P.D.FEAN.LFE.GNM...Q..L.ALAG..KTK......DIG.KY.EKQ.
Retrocopy WHQLENLSNFVKAVVSYDMNPMDLFEANNLFESGNMRHMQVSLFALAGKVKTKRPQSGVDIGIKYSEKQE

Parental  RRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPK-MQTDKPFDQTTISLQMGTNKGASQAG
          ..F.....KAGQ.VIGLQMGTNKC.SQ.GMT.YG.RRHL.DPK......P.D..TI.LQMGTNK.ASQ..
Retrocopy QNFSDAAMKAGQCVIGLQMGTNKCTSQLGMTVYGVRRHLCDPK>ITSCPPMDHSTIGLQMGTNKCASQVS

Parental  MLAPGTRRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPT
          M.APGT...IYD.KL.....DNS..SLQMG...........V.GLG....DPKYC...T
Retrocopy MTAPGTWQHIYDTKLGTDKCDNSSMSLQMGYTQ-GTNHSSQVFGLGQHICDPKYCLPGT

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain_prefrontal_cortex	0.02 RPM	37.13 RPM
SRP007412_cerebellum	0.04 RPM	64.38 RPM
SRP007412_heart	0.00 RPM	25.77 RPM
SRP007412_kidney	0.03 RPM	191.01 RPM
SRP007412_liver	0.00 RPM	31.03 RPM
SRP007412_testis	0.00 RPM	79.88 RPM

ChIP-Seq

Pan troglodytes was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ptro_1061 retrocopy.

TSS

Pan troglodytes was not studied using FANTOM5 data.

Lab

retro_ptro_1061 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ptro_1061 has 3 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_1575
Gorilla gorilla	retro_ggor_1199
Pongo abelii	retro_pabe_1299

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Parental genes homology:

Parental genes homology involve 11 parental genes, and 34 retrocopies.

Species	Parental gene accession	Retrocopies number
Callithrix jacchus	ENSCJAG00000018128	3 retrocopies	retro_cjac_1331, retro_cjac_533, retro_cjac_861,
Equus caballus	ENSECAG00000020188	1 retrocopy	retro_ecab_655,
Echinops telfairi	ENSETEG00000010095	1 retrocopy	retro_etel_638,
Macropus eugenii	ENSMEUG00000002921	2 retrocopies	retro_meug_2020, retro_meug_42,
Monodelphis domestica	ENSMODG00000004326	1 retrocopy	retro_mdom_442,
Nomascus leucogenys	ENSNLEG00000011207	5 retrocopies	retro_nleu_122, retro_nleu_1342, retro_nleu_2698, retro_nleu_480, retro_nleu_668,
Pan troglodytes	ENSPTRG00000000979	15 retrocopies	retro_ptro_1061 , retro_ptro_1149, retro_ptro_1247, retro_ptro_1447, retro_ptro_1505, retro_ptro_1554, retro_ptro_1871, retro_ptro_215, retro_ptro_2323, retro_ptro_2377, retro_ptro_2831, retro_ptro_2894, retro_ptro_3007, retro_ptro_483, retro_ptro_653,
Pan troglodytes	ENSPTRG00000001533	1 retrocopy	retro_ptro_2788,
Sarcophilus harrisii	ENSSHAG00000002670	1 retrocopy	retro_shar_567,
Tarsius syrichta	ENSTSYG00000010749	2 retrocopies	retro_tsyr_1458, retro_tsyr_1907,
Vicugna pacos	ENSVPAG00000004889	2 retrocopies	retro_vpac_190, retro_vpac_449,