RetrogeneDB ID: retro_btau_450

Retrocopy location

• Organism: Cow (Bos taurus)
• Coordinates: 11:77493039..77493712(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSBTAG00000004565
• Aliases: None
• Status: KNOWN_PSEUDOGENE

Parental gene information

• Parental gene symbol: EEF1B
• Ensembl ID: ENSBTAG00000021979
• Aliases: EEF1B2, EEF1B
• Description: Elongation factor 1-beta [Source:UniProtKB/Swiss-Prot;Acc:Q5E983]

Sequence

>retro_btau_450
ATGGGTTTTGGAGACTTGAAAAGCCCCGCTGGCCTCCAGGTGCTCAATGACTACTTGGCAGACAAGAGTTACATAGAGGG
ATATATGCCATCACAAGCAGATGTGGCAGTATTTGAAGCCGTCTCCGGGCCACCACCTGCTGACTTATGTCATGCCCTCC
ATTGGTATAATCATATCAAATCTTATGAGAAGGAAAAGGCCAGCCTGCCAGGAGTGAAGAAAGCTTTGGGCAAGTATGGC
CCTGCTAATGTGGAAGACACCGCAGAAAGTGGAGCTACAGATAGTAAAGATGATGAGGACATGGATCTCTTTGGATCTGA
TGAGGAGGAGGAAAGTGAAGAAGCCAAGAGGATAAGAGAAGAACATCTTGCCCAGTATGAGTCAAAGAAAGCCAGAAAAC
AAGCACTTGTCGCCAAGTCTTCCATCTTATTAGATGTGAAACCTTGGGATGATGCCTGATATGGCAAAACTAGAGGAATG
TGTCAGAAGCATTCAAGCAGATGGCTTGGTCTGGGGCTCTTCTAAACTAGTTCCGGTTGGGTATGGCATTAAAAAACTTC
AAACACAGTGTGTAGTTGAAGATGACAAAGTTGGGACAGACATGCTGGAGGAGCAGATCACTGCTTTCGATGAGTATGTA
CAGTTTATGGATGTGGCTGCATTCAACAAGATC

Alignment

ORF - retro_btau_450 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 92.92 %
• Parental protein coverage: 100.0 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSGPPPADLCHALRWYNHIKSYEKEKASLP
          MGFGDLKSPAGLQVLNDYLADKSYIEGY.PSQADVAVFEAVSGPPPADLCHAL.WYNHIKSYEKEKASLP
Retrocopy MGFGDLKSPAGLQVLNDYLADKSYIEGYMPSQADVAVFEAVSGPPPADLCHALHWYNHIKSYEKEKASLP

Parental  GVKKALGKYGPANVEDTTESGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKS
          GVKKALGKYGPANVEDT.ESGATDSKDD.D.DLFGSD.EEESEEAKR.REE.LAQYESKKA.K.ALVAKS
Retrocopy GVKKALGKYGPANVEDTAESGATDSKDDEDMDLFGSDEEEESEEAKRIREEHLAQYESKKARKQALVAKS

Parental  SILLDVKPWDDET-DMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITA
          SILLDVKPW.D...DMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQ.QCVVEDDKVGTDMLEEQITA
Retrocopy SILLDVKPW-DDA>DMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQTQCVVEDDKVGTDMLEEQITA

Parental  FDEYVQSMDVAAFNKI
          FDEYVQ.MDVAAFNKI
Retrocopy FDEYVQFMDVAAFNKI

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
ERP005899_liver	0.00 RPM	139.07 RPM
ERP005899_muscle	0.53 RPM	412.02 RPM
SRP017611_brain	0.00 RPM	67.01 RPM
SRP017611_kidney	0.00 RPM	153.45 RPM
SRP017611_liver	0.00 RPM	125.19 RPM
SRP030211_testis	0.05 RPM	67.16 RPM

ChIP-Seq

Bos taurus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_btau_450 retrocopy.

TSS

Bos taurus was not studied using FANTOM5 data.

Lab

retro_btau_450 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_btau_450 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 25 parental genes, and 94 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000003015	1 retrocopy	retro_amel_427,
Bos taurus	ENSBTAG00000021979	8 retrocopies	retro_btau_1127, retro_btau_1309, retro_btau_1786, retro_btau_366, retro_btau_367, retro_btau_450 , retro_btau_512, retro_btau_792,
Canis familiaris	ENSCAFG00000029635	1 retrocopy	retro_cfam_56,
Choloepus hoffmanni	ENSCHOG00000013835	1 retrocopy	retro_chof_725,
Callithrix jacchus	ENSCJAG00000005805	2 retrocopies	retro_cjac_176, retro_cjac_341,
Dipodomys ordii	ENSDORG00000003669	2 retrocopies	retro_dord_510, retro_dord_592,
Felis catus	ENSFCAG00000010239	1 retrocopy	retro_fcat_1735,
Homo sapiens	ENSG00000114942	9 retrocopies	retro_hsap_1154, retro_hsap_1500, retro_hsap_2397, retro_hsap_2883, retro_hsap_3332, retro_hsap_3480, retro_hsap_3657, retro_hsap_3903, retro_hsap_4795,
Gorilla gorilla	ENSGGOG00000007002	2 retrocopies	retro_ggor_134, retro_ggor_2000,
Macropus eugenii	ENSMEUG00000002654	8 retrocopies	retro_meug_1418, retro_meug_1846, retro_meug_1917, retro_meug_240, retro_meug_241, retro_meug_52, retro_meug_775, retro_meug_949,
Microcebus murinus	ENSMICG00000010607	3 retrocopies	retro_mmur_1067, retro_mmur_615, retro_mmur_78,
Myotis lucifugus	ENSMLUG00000016602	8 retrocopies	retro_mluc_1035, retro_mluc_1068, retro_mluc_1104, retro_mluc_1167, retro_mluc_1542, retro_mluc_1578, retro_mluc_1681, retro_mluc_1857,
Macaca mulatta	ENSMMUG00000001054	6 retrocopies	retro_mmul_1456, retro_mmul_1767, retro_mmul_1881, retro_mmul_2140, retro_mmul_894, retro_mmul_969,
Nomascus leucogenys	ENSNLEG00000007098	8 retrocopies	retro_nleu_1220, retro_nleu_1676, retro_nleu_2078, retro_nleu_2107, retro_nleu_2521, retro_nleu_2630, retro_nleu_624, retro_nleu_834,
Otolemur garnettii	ENSOGAG00000011229	3 retrocopies	retro_ogar_2628, retro_ogar_616, retro_ogar_862,
Oreochromis niloticus	ENSONIG00000017630	1 retrocopy	retro_onil_28,
Oryzias latipes	ENSORLG00000018533	1 retrocopy	retro_olat_16,
Procavia capensis	ENSPCAG00000013348	1 retrocopy	retro_pcap_154,
Pongo abelii	ENSPPYG00000013107	7 retrocopies	retro_pabe_1251, retro_pabe_1656, retro_pabe_2163, retro_pabe_2389, retro_pabe_2419, retro_pabe_2746, retro_pabe_2876,
Pteropus vampyrus	ENSPVAG00000017334	2 retrocopies	retro_pvam_1055, retro_pvam_56,
Sarcophilus harrisii	ENSSHAG00000017853	1 retrocopy	retro_shar_208,
Sus scrofa	ENSSSCG00000016128	1 retrocopy	retro_sscr_766,
Ictidomys tridecemlineatus	ENSSTOG00000005437	3 retrocopies	retro_itri_1263, retro_itri_813, retro_itri_873,
Tupaia belangeri	ENSTBEG00000005657	13 retrocopies	retro_tbel_161, retro_tbel_2046, retro_tbel_2094, retro_tbel_2298, retro_tbel_2700, retro_tbel_2776, retro_tbel_3143, retro_tbel_3541, retro_tbel_3542, retro_tbel_4050, retro_tbel_4073, retro_tbel_4205, retro_tbel_611,
Xiphophorus maculatus	ENSXMAG00000006050	1 retrocopy	retro_xmac_21,