RetrogeneDB ID: | retro_mmus_2959 | ||
Retrocopylocation | Organism: | Mouse (Mus musculus) | |
Coordinates: | 7:101338111..101338426(+) | ||
Located in intron of: | ENSMUSG00000030688 | ||
Retrocopyinformation | Ensembl ID: | None | |
Aliases: | None | ||
Status: | NOVEL | ||
Parental geneinformation | Parental gene summary: | ||
Parental gene symbol: | Rpl31 | ||
Ensembl ID: | ENSMUSG00000073702 | ||
Aliases: | None | ||
Description: | ribosomal protein L31 [Source:MGI Symbol;Acc:MGI:2149632] |
Percent Identity: | 69.52 % |
Parental protein coverage: | 83.2 % |
Number of stop codons detected: | 1 |
Number of frameshifts detected | 0 |
Parental | MAPAKKGGEKKKGRS-AINEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTRLN |
..PA.....K...R..AIN.VVTREY..N.H.RIHGVGF.KRAP.A.KE.RK..MKEM.TPDV.IDTRLN | |
Retrocopy | LGPAEWFPQKRMARRRAINDVVTREYITNVHNRIHGVGFMKRAPWAFKETRKSFMKEMRTPDVSIDTRLN |
Parental | KAVWAKGIRNVPYRIRVRLSRKRNEDEDSPNKLYT |
KAVWAK.IRNVPY.I.V.L.RK.NE.EDSPNKLYT | |
Retrocopy | KAVWAKRIRNVPYHI*VCLFRKCNEGEDSPNKLYT |
* | Stop codon |
> | Forward frameshift by one nucleotide |
< | Reverse frameshift by one nucleotide |
Library | Retrocopy expression | Parental gene expression |
---|---|---|
SRP007412_brain | 0 .30 RPM | 69 .15 RPM |
SRP007412_cerebellum | 0 .35 RPM | 44 .55 RPM |
SRP007412_heart | 0 .03 RPM | 65 .62 RPM |
SRP007412_kidney | 0 .40 RPM | 77 .00 RPM |
SRP007412_liver | 0 .90 RPM | 55 .14 RPM |
SRP007412_testis | 0 .09 RPM | 58 .23 RPM |
Experiment type: | PCR amplification |
---|---|
Forward primer: | GGTAAAGTAGGTCAGGCATC (20 nt long) |
Reverse primer: | CATACCTAGGCCTTCACACT (20 nt long) |
Anneling temperature: | 55 °C |
(Expected) product size: | 745 |
Electrophoresis gel image: | ![]() |
Additional comment: | Pfu DNA Polymerase; pooled mouse cDNA; 40 cycles of PCR |