RetrogeneDB ID: retro_mmus_546

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 10:33463968..33464332(+)
• Located in intron of: ENSMUSG00000019787

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Ssbp1
• Ensembl ID: ENSMUSG00000029911
• Aliases: Ssbp1, 2810480P10Rik, G630031O20Rik, MtSSB, mtDBP
• Description: single-stranded DNA binding protein 1 [Source:MGI Symbol;Acc:MGI:1920040]

Sequence

>retro_mmus_546
GATCGTGTTCACTTATTTGGCTGAGTAAGTCAGGACCCTGGCATGAGACAGGAGGAAGGAAAAAACCCAGTCACGATATT
TTCTCTAGCAACAAATGAGATGTGGTGATCAGGGGACAGTACAGTATACCAAATGTGTGACATCATTTAAAATCACAGCA
TGGGACAGAATATCAGTGCTTTGATCAGGTCTCAGACATGTGGCATATCAGTATGTGAAAAACGGGGGCTCCTGTATTTG
AGGAAATATATTAGGGAAAGTGGACTATAGTAAATAAATGGAAAACAACAATGGGAGGCGGCAAGCAACAACAATTATGG
CTGATAACATTATTTTTCTGAATGACCAGAGAAAATAAAAGGCT

Alignment

ORF - retro_mmus_546 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 66.39 %
• Parental protein coverage: 79.73 %
• Number of stop codons detected: 6
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  NRVQLLGRVGQDPVMRQVEGKNPVTIFSLATNEMWRSGDSEVYQMGDV-SQKTTWHRISVFRPGLRDVAY
          .RV.L.G.V.QDP.MRQ.EGKNPVTIFSLATNEMW.SGDS.VYQM.D.....T.W.RISV...GLR.VAY
Retrocopy DRVHLFG*VSQDPGMRQEEGKNPVTIFSLATNEMW*SGDSTVYQMCDI>FKITAWDRISVL*SGLRHVAY

Parental  QYVKKGARIF---VEGKVDYGEYMDKNNVRRQATTIIADNIIFLSDQTKEKA
          QYVK.G.........GKVDY...M..NN.RRQATTI.ADNIIFL.DQ.K.KA
Retrocopy QYVKNGGSCI*GNILGKVDYSK*MENNNGRRQATTIMADNIIFLNDQRK*KA

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	8.34 RPM
SRP007412_cerebellum	0.00 RPM	11.98 RPM
SRP007412_heart	0.00 RPM	17.99 RPM
SRP007412_kidney	0.00 RPM	18.02 RPM
SRP007412_liver	0.00 RPM	13.95 RPM
SRP007412_testis	0.00 RPM	5.21 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_mmus_546 was not detected

EST

No EST(s) were mapped for retro_mmus_546 retrocopy.

TSS

No TSS is located nearby retro_mmus_546 retrocopy 5' end.

Lab

retro_mmus_546 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_546 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 27 parental genes, and 99 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000015145	3 retrocopies	retro_amel_1415, retro_amel_171, retro_amel_494,
Bos taurus	ENSBTAG00000010931	2 retrocopies	retro_btau_1711, retro_btau_1838,
Canis familiaris	ENSCAFG00000003885	7 retrocopies	retro_cfam_1286, retro_cfam_2013, retro_cfam_2040, retro_cfam_303, retro_cfam_308, retro_cfam_470, retro_cfam_782,
Callithrix jacchus	ENSCJAG00000013650	3 retrocopies	retro_cjac_1951, retro_cjac_3242, retro_cjac_669,
Cavia porcellus	ENSCPOG00000003354	1 retrocopy	retro_cpor_940,
Dasypus novemcinctus	ENSDNOG00000015867	15 retrocopies	retro_dnov_1050, retro_dnov_1101, retro_dnov_1242, retro_dnov_1506, retro_dnov_1517, retro_dnov_1929, retro_dnov_1983, retro_dnov_2059, retro_dnov_2246, retro_dnov_2428, retro_dnov_2557, retro_dnov_2631, retro_dnov_480, retro_dnov_837, retro_dnov_980,
Dipodomys ordii	ENSDORG00000015161	2 retrocopies	retro_dord_367, retro_dord_40,
Equus caballus	ENSECAG00000004023	4 retrocopies	retro_ecab_764, retro_ecab_842, retro_ecab_955, retro_ecab_956,
Erinaceus europaeus	ENSEEUG00000010837	2 retrocopies	retro_eeur_155, retro_eeur_194,
Felis catus	ENSFCAG00000006568	8 retrocopies	retro_fcat_1249, retro_fcat_1565, retro_fcat_1604, retro_fcat_1640, retro_fcat_439, retro_fcat_768, retro_fcat_802, retro_fcat_805,
Homo sapiens	ENSG00000106028	1 retrocopy	retro_hsap_655,
Gorilla gorilla	ENSGGOG00000009615	3 retrocopies	retro_ggor_1762, retro_ggor_368, retro_ggor_571,
Loxodonta africana	ENSLAFG00000011526	1 retrocopy	retro_lafr_753,
Myotis lucifugus	ENSMLUG00000007133	3 retrocopies	retro_mluc_1874, retro_mluc_2297, retro_mluc_2509,
Macaca mulatta	ENSMMUG00000022442	3 retrocopies	retro_mmul_1014, retro_mmul_2437, retro_mmul_662,
Mustela putorius furo	ENSMPUG00000003257	5 retrocopies	retro_mputfur_1129, retro_mputfur_1488, retro_mputfur_538, retro_mputfur_835, retro_mputfur_893,
Mus musculus	ENSMUSG00000029911	3 retrocopies	retro_mmus_2762, retro_mmus_546 , retro_mmus_703,
Nomascus leucogenys	ENSNLEG00000002361	2 retrocopies	retro_nleu_2258, retro_nleu_61,
Otolemur garnettii	ENSOGAG00000016661	9 retrocopies	retro_ogar_1021, retro_ogar_1085, retro_ogar_1729, retro_ogar_1806, retro_ogar_1958, retro_ogar_2345, retro_ogar_2572, retro_ogar_3020, retro_ogar_757,
Procavia capensis	ENSPCAG00000003383	1 retrocopy	retro_pcap_371,
Pongo abelii	ENSPPYG00000018068	4 retrocopies	retro_pabe_2103, retro_pabe_490, retro_pabe_578, retro_pabe_661,
Pan troglodytes	ENSPTRG00000019778	3 retrocopies	retro_ptro_132, retro_ptro_1695, retro_ptro_476,
Pteropus vampyrus	ENSPVAG00000006221	2 retrocopies	retro_pvam_493, retro_pvam_525,
Rattus norvegicus	ENSRNOG00000012100	4 retrocopies	retro_rnor_1758, retro_rnor_2001, retro_rnor_2631, retro_rnor_915,
Sus scrofa	ENSSSCG00000016488	3 retrocopies	retro_sscr_1099, retro_sscr_658, retro_sscr_915,
Ictidomys tridecemlineatus	ENSSTOG00000007007	4 retrocopies	retro_itri_1307, retro_itri_1481, retro_itri_1490, retro_itri_902,
Vicugna pacos	ENSVPAG00000003679	1 retrocopy	retro_vpac_71,