RetrogeneDB ID: retro_ptro_663

Retrocopy location

• Organism: Chimpanzee (Pan troglodytes)
• Coordinates: 12:8672902..8673514(+)
• Located in intron of: ENSPTRG00000029793

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: RPL15
• Ensembl ID: ENSPTRG00000014695
• Aliases: None
• Description: ribosomal protein L15 [Source:HGNC Symbol;Acc:10306]

Sequence

>retro_ptro_663
ATGGGTGCATACAAGTACATCCAGGAGCTATGGAGAAAGAAGCAGTCTGATGTCATGAGCTTTCTTCTGAGGGTCCACTG
CTGACAGTACCGCCAGCTCTCTGCTCTCCACAGGGCTCCCCACCCCACCTGGCCTGATAAAGCGGGCCGACTGGGCTACA
AGGCCAAACAAGGTTACCTTATATATAGGATTCGTGTTCGCCCTGGTGGCCAAAAACGCCCAGTTCCTAAGGGTACAACT
TACCGCAAGCCTGTCTATCACGGTGTTAACCAGCCAAAGTTTGCTCCAAACCTTCAGTCTGTTGCAGAGGAGCGAGCTGG
ATGCCACTGTGGGGCTCTAAGAGTCCCAAATTCTTACTGCGTTGGTGAAGATTCCACATAAAAATTTTTGGGGGTTATCC
TCATCGATCCATTTTATAAAGCTATCAGAAGAAATCCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGGGAG
ATGTGTGGGCTGACATCTGCAGGCTGAAAGAGCCGTGGCCTTGGAAAGGGCCATAAGTTCCACCACACTATTTGTGGTTC
TCATCTGGCAGATTGGAGAATGTGCAATACTCTCCAGCTCCACCGTTACCGC

Alignment

ORF - retro_ptro_663 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 85.29 %
• Parental protein coverage: 100. %
• Number of stop codons detected: 3
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGG
          MGAYKYIQELWRKKQSDVM.FLLRV.C.QYRQLSALHRAP.PT.PDKA.RLGYKAKQGY.IYRIRVR.GG
Retrocopy MGAYKYIQELWRKKQSDVMSFLLRVHC*QYRQLSALHRAPHPTWPDKAGRLGYKAKQGYLIYRIRVRPGG

Parental  RKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHK
          .KRPVPKG.TY.KPV.HGVNQ.KFA..LQSVAEERAG.HCGALRV.NSY.VGEDST.KF..VILIDPF.K
Retrocopy QKRPVPKGTTYRKPVYHGVNQPKFAPNLQSVAEERAGCHCGALRVPNSYCVGEDST*KFLGVILIDPFYK

Parental  AIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR
          AIRRNPDTQWITKPVHKHREM.GLTSAG.KSRGLGKGHKFHHTI.GS..A.WR..NTLQLHRYR
Retrocopy AIRRNPDTQWITKPVHKHREMCGLTSAG*KSRGLGKGHKFHHTICGSHLADWRMCNTLQLHRYR

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain_prefrontal_cortex	0.02 RPM	384.18 RPM
SRP007412_cerebellum	0.04 RPM	392.25 RPM
SRP007412_heart	0.00 RPM	133.81 RPM
SRP007412_kidney	0.03 RPM	397.82 RPM
SRP007412_liver	0.10 RPM	190.71 RPM
SRP007412_testis	0.00 RPM	185.79 RPM

ChIP-Seq

Pan troglodytes was not studied using ChIP-Seq data.

EST

2 EST(s) were mapped to retro_ptro_663 retrocopy

EST ID	Start	End	Identity	Match	Mis-match	Score
CB295009	8672901	8673104	96.1	195	8	187
CB295010	8672901	8673088	95.8	179	8	171

TSS

Pan troglodytes was not studied using FANTOM5 data.

Lab

retro_ptro_663 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ptro_663 has 2 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_957
Gorilla gorilla	retro_ggor_771

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Parental genes homology:

Parental genes homology involve 9 parental genes, and 83 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000003497	6 retrocopies	retro_amel_1281, retro_amel_1470, retro_amel_1519, retro_amel_516, retro_amel_548, retro_amel_712,
Callithrix jacchus	ENSCJAG00000003810	23 retrocopies	retro_cjac_1052, retro_cjac_1270, retro_cjac_1316, retro_cjac_1553, retro_cjac_1578, retro_cjac_1625, retro_cjac_1668, retro_cjac_1741, retro_cjac_2143, retro_cjac_2330, retro_cjac_2518, retro_cjac_2532, retro_cjac_2599, retro_cjac_2821, retro_cjac_2907, retro_cjac_3807, retro_cjac_4185, retro_cjac_506, retro_cjac_585, retro_cjac_712, retro_cjac_734, retro_cjac_783, retro_cjac_890,
Equus caballus	ENSECAG00000010157	1 retrocopy	retro_ecab_38,
Procavia capensis	ENSPCAG00000011995	1 retrocopy	retro_pcap_265,
Pongo abelii	ENSPPYG00000014065	18 retrocopies	retro_pabe_1073, retro_pabe_1201, retro_pabe_1480, retro_pabe_1896, retro_pabe_2049, retro_pabe_2377, retro_pabe_2433, retro_pabe_2467, retro_pabe_2562, retro_pabe_3004, retro_pabe_3028, retro_pabe_3098, retro_pabe_561, retro_pabe_597, retro_pabe_642, retro_pabe_705, retro_pabe_801, retro_pabe_816,
Pan troglodytes	ENSPTRG00000014695	14 retrocopies	retro_ptro_1178, retro_ptro_1646, retro_ptro_1942, retro_ptro_2064, retro_ptro_2090, retro_ptro_2483, retro_ptro_2572, retro_ptro_435, retro_ptro_459, retro_ptro_550, retro_ptro_646, retro_ptro_663 , retro_ptro_886, retro_ptro_991,
Pteropus vampyrus	ENSPVAG00000001771	13 retrocopies	retro_pvam_1016, retro_pvam_1067, retro_pvam_1178, retro_pvam_1280, retro_pvam_1386, retro_pvam_1422, retro_pvam_176, retro_pvam_338, retro_pvam_397, retro_pvam_538, retro_pvam_634, retro_pvam_766, retro_pvam_83,
Tursiops truncatus	ENSTTRG00000007244	5 retrocopies	retro_ttru_1305, retro_ttru_1358, retro_ttru_280, retro_ttru_920, retro_ttru_987,
Vicugna pacos	ENSVPAG00000008573	2 retrocopies	retro_vpac_34, retro_vpac_522,