RetrogeneDB ID: retro_mmus_868

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 12:61579676..61580175(+)
• Located in intron of: ENSMUSG00000035653

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Eif4e
• Ensembl ID: ENSMUSG00000028156
• Aliases: Eif4e, EG668879, Eif4e-ps, If4e, eIF-4E
• Description: eukaryotic translation initiation factor 4E [Source:MGI Symbol;Acc:MGI:95305]

Sequence

>retro_mmus_868
GATAAAAGCAAAACTCGGCAAGCAAACTTTCGATTGATCCCTAAATTTGACACTGTTGAAGACTTTTGGGCTCTATACAA
CTATATCCAGTTGTCTAGTAATTTAATGTCTGGCTGTGACTACTCACTTCTTAAGGATGGCATTGAACCTATGTGGGAAG
ATGAGTAAAACAAAGAGGAGGACGATGGCCAATTACATTGAACAAGCAGCAGAGACAAAGTGACTTCTGTCACTTTTGGC
TAGAAACACTGCTGTGCCTTATTGGAGAATCTTTTGATGACTACAGTGATGATGTGTGTGGAGCTGTTGTTAATGTTAGA
GTTAAAGTTGCTAAGAGAGCAATATGGACTACTGAATGTGAAAACAGAGACACAGTCACACCCATAGGGAGGGTATACAA
GGAAAGGTTAGGACTTCCTCCGAAGATAGTGATTGGTTATCAGTCCCACACAGACACAGCTCAAAGAGTGGCTCCACAAC
TAAAAATAGGTTTGTTGTT

Alignment

ORF - retro_mmus_868 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 86.98 %
• Parental protein coverage: 76.96 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  DKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNK-RGGRWLITLNK
          DKSKT.QAN.RLI.KFDTVEDFWALYN.IQLSSNLM.GCDYSL.KDGIEPMWEDE.NK.RGGRW.ITLNK
Retrocopy DKSKTRQANFRLIPKFDTVEDFWALYNYIQLSSNLMSGCDYSLLKDGIEPMWEDE*NK<RGGRWPITLNK

Parental  QQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGL
          QQR.SD...FWLETLLCLIGESFDDYSDDVCGAVVNVR.K..K.AIWTTECENRD.VT.IGRVYKERLGL
Retrocopy QQRQSDFCHFWLETLLCLIGESFDDYSDDVCGAVVNVRVKVAKRAIWTTECENRDTVTPIGRVYKERLGL

Parental  PPKIVIGYQSHADTA-TKSGSTTKNRFVV
          PPKIVIGYQSH.DTA..KSGSTTKNRFVV
Retrocopy PPKIVIGYQSHTDTA<SKSGSTTKNRFVV

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.21 RPM	55.63 RPM
SRP007412_cerebellum	0.17 RPM	43.68 RPM
SRP007412_heart	0.06 RPM	80.47 RPM
SRP007412_kidney	0.04 RPM	55.05 RPM
SRP007412_liver	0.00 RPM	25.45 RPM
SRP007412_testis	0.09 RPM	113.80 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_mmus_868 was not detected

EST

No EST(s) were mapped for retro_mmus_868 retrocopy.

TSS

No TSS is located nearby retro_mmus_868 retrocopy 5' end.

Lab

retro_mmus_868 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_868 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 24 parental genes, and 80 retrocopies.

Species	Parental gene accession	Retrocopies number
Choloepus hoffmanni	ENSCHOG00000009450	9 retrocopies	retro_chof_1177, retro_chof_1219, retro_chof_124, retro_chof_1319, retro_chof_1551, retro_chof_200, retro_chof_604, retro_chof_918, retro_chof_992,
Callithrix jacchus	ENSCJAG00000007151	2 retrocopies	retro_cjac_1373, retro_cjac_2097,
Cavia porcellus	ENSCPOG00000005383	4 retrocopies	retro_cpor_1033, retro_cpor_1480, retro_cpor_169, retro_cpor_501,
Dasypus novemcinctus	ENSDNOG00000013712	5 retrocopies	retro_dnov_1722, retro_dnov_2378, retro_dnov_2570, retro_dnov_353, retro_dnov_638,
Echinops telfairi	ENSETEG00000007347	3 retrocopies	retro_etel_1285, retro_etel_1449, retro_etel_499,
Homo sapiens	ENSG00000151247	4 retrocopies	retro_hsap_1843, retro_hsap_2409, retro_hsap_2894, retro_hsap_3935,
Gorilla gorilla	ENSGGOG00000004718	3 retrocopies	retro_ggor_1497, retro_ggor_2005, retro_ggor_2160,
Macropus eugenii	ENSMEUG00000011104	3 retrocopies	retro_meug_2087, retro_meug_2114, retro_meug_428,
Microcebus murinus	ENSMICG00000001482	1 retrocopy	retro_mmur_1195,
Myotis lucifugus	ENSMLUG00000005035	3 retrocopies	retro_mluc_1519, retro_mluc_24, retro_mluc_474,
Macaca mulatta	ENSMMUG00000011066	2 retrocopies	retro_mmul_1451, retro_mmul_601,
Monodelphis domestica	ENSMODG00000020716	1 retrocopy	retro_mdom_701,
Mustela putorius furo	ENSMPUG00000002041	1 retrocopy	retro_mputfur_37,
Mus musculus	ENSMUSG00000028156	6 retrocopies	retro_mmus_1059, retro_mmus_1478, retro_mmus_1872, retro_mmus_2362, retro_mmus_3462, retro_mmus_868 ,
Mus musculus	ENSMUSG00000074895	1 retrocopy	retro_mmus_510,
Oryctolagus cuniculus	ENSOCUG00000001456	3 retrocopies	retro_ocun_1760, retro_ocun_596, retro_ocun_810,
Otolemur garnettii	ENSOGAG00000001131	1 retrocopy	retro_ogar_259,
Pongo abelii	ENSPPYG00000014944	3 retrocopies	retro_pabe_1774, retro_pabe_2396, retro_pabe_3244,
Pan troglodytes	ENSPTRG00000016301	4 retrocopies	retro_ptro_1159, retro_ptro_1386, retro_ptro_1957, retro_ptro_2672,
Rattus norvegicus	ENSRNOG00000013183	11 retrocopies	retro_rnor_1437, retro_rnor_1534, retro_rnor_1544, retro_rnor_1545, retro_rnor_1853, retro_rnor_1854, retro_rnor_2017, retro_rnor_2159, retro_rnor_653, retro_rnor_781, retro_rnor_783,
Sorex araneus	ENSSARG00000007821	1 retrocopy	retro_sara_285,
Ictidomys tridecemlineatus	ENSSTOG00000002230	5 retrocopies	retro_itri_1548, retro_itri_1549, retro_itri_1818, retro_itri_1819, retro_itri_1863,
Tarsius syrichta	ENSTSYG00000010862	3 retrocopies	retro_tsyr_1077, retro_tsyr_1442, retro_tsyr_2169,
Drosophila melanogaster	FBgn0035709	1 retrocopy	retro_dmel_68,