RetrogeneDB ID: retro_acar_124

Retrocopy location

• Organism: Anole lizard (Anolis carolinensis)
• Coordinates: 4:111886101..111886715(+)
• Located in intron of: ENSACAG00000002424

Retrocopy information

• Ensembl ID: ENSACAG00000002593
• Aliases: None
• Status: KNOWN_PSEUDOGENE

Parental gene information

• Parental gene symbol: RPS8
• Ensembl ID: ENSACAG00000015697
• Aliases: None
• Description: 40S ribosomal protein S8 [Source:UniProtKB/TrEMBL;Acc:G1KRF8]

Sequence

>retro_acar_124
ATCTCCAGGGACAACTGACACAAGCACTGCAAAACTGGGGAGGAAGCGCAAACCCTACCATAAGAAGAGAAAGTATGAGT
TGGGGCGACCCCCTGCCAATACCAAAATTGGCCCCCGGAGGATTCACACAGTAAGGGTATGTGGTAGAAATAAAAAATAC
CGTGCTCTGCATCTGGATGTTGGAAACTTCTCTTGGGGATCTGAATGCTGCACCCGTAAAACTAGAATCATTGATGTAGT
GTACAATGCCTCCAACAATGAGCTGGTGCGCACAAAGACCCTGGTCAAAAACTGCATTGTTCTCATTGACAGCACACCAT
ACCGTCAGTGGTATGAAGCCCACTATGCATTACCCCTTGGCCGCAAAAAGGGCGCCAAGCTGACATGAGGAGAAAGAAAT
CCTAAACAAAAAGTGTTCAAAGAAAATTCAGAAGTATGATGAGCGGAAAAAGAATGCTAAGATCAGTAGTCTTCTGGAAG
AGCAGTTCCAGCAAGGAAAACTGCTTGCTTGCATTGCCTCCAGGCCTGGACAATGTGGTGGAGCAGATGGCTGCCTCCCA
GAAGGCAAAGAACTTGAATTCTATCTGAGTAAGATCAAGGCCAGGAGAGGCAAA

Alignment

ORF - retro_acar_124 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 91.35 %
• Parental protein coverage: 99.04 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  ISRDNWHKRRKTGG-KRKPYHKKRKYELGRPPANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSEC
          ISRDN.HK..KTG..KRKPYHKKRKYELGRPPANTKIGPRRIHTVRV.G.NKKYRAL.LDVGNFSWGSEC
Retrocopy ISRDN*HKHCKTGE>KRKPYHKKRKYELGRPPANTKIGPRRIHTVRVCGRNKKYRALHLDVGNFSWGSEC

Parental  CTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYEAHYALPLGRKKGAKLT-PEEEEILNKK
          CTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYEAHYALPLGRKKGAKLT..E.E.ILNKK
Retrocopy CTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYEAHYALPLGRKKGAKLT>EEKE-ILNKK

Parental  RSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYLLEGKELEFYLRKIKARRGK
          .SKKIQ.KYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCG.ADG.L.EGKELEFYL.KIKARRGK
Retrocopy CSKKIQ-KYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGGADGCLPEGKELEFYLSKIKARRGK

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP009831_adrenal	6.73 RPM	2721.79 RPM
SRP009831_brain	0.19 RPM	325.84 RPM
SRP009831_dewlap	0.17 RPM	533.83 RPM
SRP009831_embryo	1.84 RPM	1198.93 RPM
SRP009831_heart	0.05 RPM	703.42 RPM
SRP009831_liver	0.04 RPM	1393.50 RPM
SRP009831_lung	0.10 RPM	603.87 RPM
SRP009831_ovary	0.04 RPM	338.90 RPM
SRP009831_skeletal_muscle	0.00 RPM	417.10 RPM

ChIP-Seq

Anolis carolinensis was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_acar_124 retrocopy.

TSS

Anolis carolinensis was not studied using FANTOM5 data.

Lab

retro_acar_124 was not experimentally validated.

Homology

Retrocopy orthology:

Anolis carolinensis does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 25 parental genes, and 161 retrocopies.

Species	Parental gene accession	Retrocopies number
Anolis carolinensis	ENSACAG00000015697	1 retrocopy	retro_acar_124 ,
Canis familiaris	ENSCAFG00000004745	8 retrocopies	retro_cfam_1336, retro_cfam_1662, retro_cfam_1730, retro_cfam_2139, retro_cfam_2177, retro_cfam_261, retro_cfam_589, retro_cfam_791,
Choloepus hoffmanni	ENSCHOG00000008785	10 retrocopies	retro_chof_1197, retro_chof_1258, retro_chof_1466, retro_chof_1638, retro_chof_2066, retro_chof_2617, retro_chof_2633, retro_chof_274, retro_chof_331, retro_chof_951,
Callithrix jacchus	ENSCJAG00000004197	5 retrocopies	retro_cjac_1068, retro_cjac_181, retro_cjac_3138, retro_cjac_6, retro_cjac_951,
Dasypus novemcinctus	ENSDNOG00000025236	3 retrocopies	retro_dnov_1717, retro_dnov_802, retro_dnov_908,
Dipodomys ordii	ENSDORG00000009287	1 retrocopy	retro_dord_429,
Echinops telfairi	ENSETEG00000004002	13 retrocopies	retro_etel_1000, retro_etel_1063, retro_etel_1201, retro_etel_147, retro_etel_1495, retro_etel_1733, retro_etel_1864, retro_etel_1941, retro_etel_2050, retro_etel_2060, retro_etel_302, retro_etel_368, retro_etel_395,
Felis catus	ENSFCAG00000022996	4 retrocopies	retro_fcat_1222, retro_fcat_200, retro_fcat_456, retro_fcat_962,
Homo sapiens	ENSG00000142937	8 retrocopies	retro_hsap_1467, retro_hsap_1536, retro_hsap_1942, retro_hsap_2034, retro_hsap_2791, retro_hsap_3158, retro_hsap_3410, retro_hsap_635,
Gorilla gorilla	ENSGGOG00000005645	5 retrocopies	retro_ggor_1127, retro_ggor_1170, retro_ggor_1357, retro_ggor_1465, retro_ggor_556,
Loxodonta africana	ENSLAFG00000027596	3 retrocopies	retro_lafr_531, retro_lafr_647, retro_lafr_904,
Microcebus murinus	ENSMICG00000008024	4 retrocopies	retro_mmur_1180, retro_mmur_1434, retro_mmur_1501, retro_mmur_380,
Myotis lucifugus	ENSMLUG00000001559	30 retrocopies	retro_mluc_1114, retro_mluc_1132, retro_mluc_1165, retro_mluc_1277, retro_mluc_1288, retro_mluc_1418, retro_mluc_1467, retro_mluc_1476, retro_mluc_1486, retro_mluc_1634, retro_mluc_1656, retro_mluc_1689, retro_mluc_1808, retro_mluc_1911, retro_mluc_1938, retro_mluc_2043, retro_mluc_2055, retro_mluc_2085, retro_mluc_2111, retro_mluc_2153, retro_mluc_2182, retro_mluc_2209, retro_mluc_2414, retro_mluc_460, retro_mluc_662, retro_mluc_730, retro_mluc_773, retro_mluc_820, retro_mluc_821, retro_mluc_906,
Macaca mulatta	ENSMMUG00000015270	2 retrocopies	retro_mmul_275, retro_mmul_70,
Monodelphis domestica	ENSMODG00000028389	1 retrocopy	retro_mdom_998,
Mus musculus	ENSMUSG00000047675	33 retrocopies	retro_mmus_1003, retro_mmus_1067, retro_mmus_1184, retro_mmus_1694, retro_mmus_1755, retro_mmus_1796, retro_mmus_2001, retro_mmus_2002, retro_mmus_2003, retro_mmus_2031, retro_mmus_2132, retro_mmus_2173, retro_mmus_2264, retro_mmus_2732, retro_mmus_2772, retro_mmus_2847, retro_mmus_2861, retro_mmus_293, retro_mmus_2956, retro_mmus_2998, retro_mmus_3018, retro_mmus_3052, retro_mmus_3057, retro_mmus_3279, retro_mmus_3307, retro_mmus_3521, retro_mmus_364, retro_mmus_392, retro_mmus_548, retro_mmus_559, retro_mmus_596, retro_mmus_646, retro_mmus_876,
Nomascus leucogenys	ENSNLEG00000014147	1 retrocopy	retro_nleu_34,
Oryctolagus cuniculus	ENSOCUG00000012476	1 retrocopy	retro_ocun_168,
Otolemur garnettii	ENSOGAG00000006922	16 retrocopies	retro_ogar_1156, retro_ogar_1746, retro_ogar_1895, retro_ogar_2501, retro_ogar_2529, retro_ogar_2556, retro_ogar_2699, retro_ogar_2723, retro_ogar_2947, retro_ogar_308, retro_ogar_3291, retro_ogar_3436, retro_ogar_394, retro_ogar_521, retro_ogar_730, retro_ogar_805,
Pongo abelii	ENSPPYG00000001426	1 retrocopy	retro_pabe_147,
Pan troglodytes	ENSPTRG00000000659	1 retrocopy	retro_ptro_30,
Rattus norvegicus	ENSRNOG00000018768	2 retrocopies	retro_rnor_222, retro_rnor_235,
Sorex araneus	ENSSARG00000000899	1 retrocopy	retro_sara_462,
Ictidomys tridecemlineatus	ENSSTOG00000006322	3 retrocopies	retro_itri_1337, retro_itri_1380, retro_itri_750,
Tursiops truncatus	ENSTTRG00000003388	4 retrocopies	retro_ttru_1839, retro_ttru_282, retro_ttru_300, retro_ttru_344,