RetrogeneDB ID: retro_ecab_445

Retrocopy location

• Organism: Horse (Equus caballus)
• Coordinates: 18:33567783..33568367(-)
• Located in intron of: ENSECAG00000012679

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: NSA2
• Ensembl ID: ENSECAG00000005528
• Aliases: None
• Description: NSA2 ribosome biogenesis homolog (S. cerevisiae) [Source:HGNC Symbol;Acc:30728]

Sequence

>retro_ecab_445
ACTACCAAGATGCATGAAAAGAGAAATACCAAACAAAAGAATGATGAAAAGACTCCACAGGAACAGTACCTATCTATCTG
CTGGACACAGAGGGACAGTCTGGAGCTAAAATACTTTCAAATATGGTTAAACAAAAACAAAAAGAAAAAGCAGGAATAAG
GAAAGACCTTCTACCCAAGGTTCACGCTCAGGGAGAAACAAATCTTAAAAGTTATTTGAACAGGAAAAGAGAAAGAACAA
AGCATCAAAGAGGTTGGTTACTAAAGCCTGCTCTGTTGGAAATGGCTTTTTGTGAAAACCACCTAAATATGAAAGATTCA
TTAGGCCAATGGGCTTATGTTTCAAGGCGGCCCATGTAATATATTCTGAAATCAAAGTCACCTTTTGCCTGTAAATACAC
GGTGTAAAGAAAAATCTTTTGTCTCCACTATATACAACTTTTGGGTATTACTACCAAAGATATGGTCATTGAGGTGAATG
TGAGCAAGTGAGGCCTTGTGAAAGGAGACAAGGTTATTCGGGGGCAATATGCCTAGGTTACTAACAATCATGAAAATGAC
GGATGCATAAATGTAGTCTTATTG

Alignment

ORF - retro_ecab_445 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 67.84 %
• Parental protein coverage: 75.38 %
• Number of stop codons detected: 4
• Number of frameshifts detected: 3

Retrocopy - Parental Gene Alignment:

Parental  TIKMHEKRNTKQKNDEKTPQ-GAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEV
          T.KMHEKRNTKQKNDEKTPQ.G.VP.YLLD.EGQS.AK.LSNM........AG.....LPKV.AQGET..
Retrocopy TTKMHEKRNTKQKNDEKTPQ<GTVPIYLLDTEGQSGAKILSNMVXXXXXXXAGIRKDLLPKVHAQGETNL

Parental  L-KVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKK
          ..K.....KRK.KA.KR.VTK.C.VG.GF..KPPKYERFIRPMGL.FK.AHV...E.K.TFCL.I.GVKK
Retrocopy K<KLFEQEKRKNKASKRLVTKACSVGNGFL*KPPKYERFIRPMGLCFKAAHVIYSEIKVTFCL*IHGVKK

Parental  NPSSPLYTT-LGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINAVLL
          N..SPLYTT.LG..TK..VIEVNVS..GLV..G.KVI.G.YA.VTNN.ENDGCIN.VLL
Retrocopy NLLSPLYTT>LGITTKDMVIEVNVSK*GLV-KGDKVIRGQYA*VTNNHENDGCINVVLL

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP021940_articular_cartilage	0.41 RPM	44.21 RPM
SRP021940_cerebellum	0.00 RPM	13.66 RPM
SRP021940_embryo	0.00 RPM	51.43 RPM
SRP021940_placental_villous	0.53 RPM	34.01 RPM
SRP021940_synovial_membrane	0.41 RPM	34.15 RPM
SRP021940_testis	0.13 RPM	29.41 RPM

ChIP-Seq

Equus caballus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ecab_445 retrocopy.

TSS

Equus caballus was not studied using FANTOM5 data.

Lab

retro_ecab_445 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ecab_445 has 0 orthologous retrocopies within eutheria group .

---

Parental genes homology:

Parental genes homology involve 17 parental genes, and 148 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000003066	3 retrocopies	retro_btau_326, retro_btau_931, retro_btau_949,
Canis familiaris	ENSCAFG00000008311	4 retrocopies	retro_cfam_1292, retro_cfam_361, retro_cfam_49, retro_cfam_64,
Callithrix jacchus	ENSCJAG00000016874	1 retrocopy	retro_cjac_116,
Equus caballus	ENSECAG00000005528	1 retrocopy	retro_ecab_445 ,
Echinops telfairi	ENSETEG00000004095	13 retrocopies	retro_etel_1086, retro_etel_1479, retro_etel_1786, retro_etel_2030, retro_etel_2112, retro_etel_2116, retro_etel_2197, retro_etel_359, retro_etel_613, retro_etel_729, retro_etel_890, retro_etel_940, retro_etel_988,
Felis catus	ENSFCAG00000011978	1 retrocopy	retro_fcat_98,
Loxodonta africana	ENSLAFG00000009910	4 retrocopies	retro_lafr_253, retro_lafr_455, retro_lafr_620, retro_lafr_714,
Macropus eugenii	ENSMEUG00000015798	3 retrocopies	retro_meug_1191, retro_meug_1408, retro_meug_161,
Myotis lucifugus	ENSMLUG00000014464	4 retrocopies	retro_mluc_1596, retro_mluc_1664, retro_mluc_757, retro_mluc_917,
Monodelphis domestica	ENSMODG00000001900	4 retrocopies	retro_mdom_115, retro_mdom_1702, retro_mdom_581, retro_mdom_592,
Mus musculus	ENSMUSG00000060739	25 retrocopies	retro_mmus_1121, retro_mmus_1329, retro_mmus_1472, retro_mmus_1558, retro_mmus_1587, retro_mmus_1751, retro_mmus_1785, retro_mmus_1819, retro_mmus_1890, retro_mmus_2426, retro_mmus_2617, retro_mmus_2706, retro_mmus_2806, retro_mmus_3105, retro_mmus_3246, retro_mmus_3302, retro_mmus_3340, retro_mmus_3485, retro_mmus_3671, retro_mmus_3713, retro_mmus_402, retro_mmus_518, retro_mmus_674, retro_mmus_690, retro_mmus_890,
Oryctolagus cuniculus	ENSOCUG00000006536	6 retrocopies	retro_ocun_149, retro_ocun_1591, retro_ocun_1602, retro_ocun_306, retro_ocun_658, retro_ocun_76,
Pteropus vampyrus	ENSPVAG00000005396	2 retrocopies	retro_pvam_1352, retro_pvam_476,
Rattus norvegicus	ENSRNOG00000016530	15 retrocopies	retro_rnor_1074, retro_rnor_1317, retro_rnor_1366, retro_rnor_1921, retro_rnor_2630, retro_rnor_2745, retro_rnor_2767, retro_rnor_2817, retro_rnor_2820, retro_rnor_2852, retro_rnor_2877, retro_rnor_2964, retro_rnor_618, retro_rnor_945, retro_rnor_951,
Sorex araneus	ENSSARG00000009424	4 retrocopies	retro_sara_555, retro_sara_589, retro_sara_620, retro_sara_703,
Sus scrofa	ENSSSCG00000014075	2 retrocopies	retro_sscr_287, retro_sscr_288,
Tupaia belangeri	ENSTBEG00000012978	56 retrocopies	retro_tbel_1062, retro_tbel_1078, retro_tbel_1141, retro_tbel_1317, retro_tbel_1329, retro_tbel_1476, retro_tbel_152, retro_tbel_1548, retro_tbel_1569, retro_tbel_1627, retro_tbel_1733, retro_tbel_1762, retro_tbel_1778, retro_tbel_179, retro_tbel_1845, retro_tbel_1940, retro_tbel_2102, retro_tbel_2113, retro_tbel_2138, retro_tbel_2220, retro_tbel_229, retro_tbel_254, retro_tbel_2586, retro_tbel_2757, retro_tbel_2784, retro_tbel_3016, retro_tbel_3040, retro_tbel_3116, retro_tbel_3264, retro_tbel_3298, retro_tbel_3417, retro_tbel_3514, retro_tbel_3601, retro_tbel_3650, retro_tbel_3724, retro_tbel_3736, retro_tbel_3763, retro_tbel_3795, retro_tbel_4028, retro_tbel_406, retro_tbel_4104, retro_tbel_4169, retro_tbel_4270, retro_tbel_430, retro_tbel_4401, retro_tbel_4422, retro_tbel_4491, retro_tbel_4503, retro_tbel_4513, retro_tbel_4642, retro_tbel_4689, retro_tbel_535, retro_tbel_670, retro_tbel_721, retro_tbel_897, retro_tbel_91,